Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach

Detalhes bibliográficos
Autor(a) principal: Crepaldi, Frederico
Data de Publicação: 2019
Outros Autores: Toledo, Juliano Simões de, armo, Anderson Oliveira do C., Machado, Leopoldo Ferreira Marques, Brito, Daniela Diniz Viana de, Serufo, Angela Vieira, Almeida, Ana Paula Martins, Oliveira, Leandro Gonzaga de, Ricotta, Tiago Queiroga Nery, Moreira, Douglas de Souza, Murta, Silvane Maria Fonseca, Diniz, Ariane Barros, Menezes, Gustavo Batista, López-Gonzálvez, Ángeles, Barbas, Coral, Fernandes, Ana Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39723
Resumo: Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil / Centro de Metabolómica y Bioanálisis. Unidad Metabolómica. Interacciones y Bioanálisis (UMIB), Universidad CEU. San Pablo, Boadilla del Monte, Spain.
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spelling Crepaldi, FredericoToledo, Juliano Simões dearmo, Anderson Oliveira do C.Machado, Leopoldo Ferreira MarquesBrito, Daniela Diniz Viana deSerufo, Angela VieiraAlmeida, Ana Paula MartinsOliveira, Leandro Gonzaga deRicotta, Tiago Queiroga NeryMoreira, Douglas de SouzaMurta, Silvane Maria FonsecaDiniz, Ariane BarrosMenezes, Gustavo BatistaLópez-Gonzálvez, ÁngelesBarbas, CoralFernandes, Ana Paula2020-02-04T17:13:46Z2020-02-04T17:13:46Z2019CREPALDI, Frederico et al. Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-22, 4 Dec. 2019.2235-2988https://www.arca.fiocruz.br/handle/icict/3972310.3389/fcimb.2019.00403engFrontiers Media SAL. amazonensisL. amazonensisAttenuationInflammationMetabolic pathwaysMetacyclogenesisMultiplatform metabolomic fingerprintMapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil / Centro de Metabolómica y Bioanálisis. Unidad Metabolómica. Interacciones y Bioanálisis (UMIB), Universidad CEU. San Pablo, Boadilla del Monte, Spain.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil / Centro de Metabolómica y Bioanálisis. Unidad Metabolómica. Interacciones y Bioanálisis (UMIB), Universidad CEU. San Pablo, Boadilla del Monte, Spain.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Laboratório de Biotecnologia e Marcadores Moleculares. Belo Horizonte, MG, Brasil.Manchester Institute of Biotechnology. The University of Manchester. Manchester, United Kingdom.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil.Centro de Metabolómica y Bioanálisis. Unidad Metabolómica. Interacciones y Bioanálisis (UMIB), Universidad CEU. San Pablo, Boadilla del Monte, Spain.Centro de Metabolómica y Bioanálisis. Unidad Metabolómica. Interacciones y Bioanálisis (UMIB), Universidad CEU. San Pablo, Boadilla del Monte, Spain.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Leishmaniases are widespread neglected diseases with an incidence of 1.6 million new cases and 40 thousand deaths per year. Leishmania parasites may show distinct, species-specific patterns of virulence that lead to different clinical manifestations. It is well known that successive in vitro passages (SIVP) lead to the attenuation of virulence, but neither the metabolism nor the pathways involved in these processes are well understood. Herein, promastigotes of a virulent L. amazonensis strain recently isolated from mice was compared to SIVP derived and attenuated promastigotes, submitted to 10, 40, and 60 axenic passages and named R10, R40, and R60, respectively. In vitro assays and in vivo tests were performed to characterize and confirmed the attenuation profiles. A metabolomic fingerprint comparison of R0, R10, and R60 was performed by means of capillary electrophoresis, liquid and gas chromatography coupled to mass spectrometry. To validate the metabolomic data, qPCR for selected loci, flow cytometry to measure aPS exposure, sensitivity to antimony tartrate and ROS production assays were conducted. The 65 identified metabolites were clustered in biochemical categories and mapped in eight metabolic pathways: ABC transporters; fatty acid biosynthesis; glycine, serine and threonine metabolism; β-alanine metabolism; glutathione metabolism; oxidative phosphorylation; glycerophospholipid metabolism and lysine degradation. The obtained metabolomic data correlated with previous proteomic findings of the SVIP parasites and the gene expression of 13 selected targets. Late SIVP cultures were more sensitive to SbIII produced more ROS and exposed less phosphatidylserine in their surface. The correspondent pathways were connected to build a biochemical map of the most significant alterations involved with the process of attenuation of L. amazonensis. Overall, the reported data pointed out to a very dynamic and continuous metabolic reprogramming process, accompanied by changes in energetic, lipid and redox metabolisms, membrane remodeling and reshaping of parasite-host cells interactions, causing impacts in chemotaxis, host inflammatory responses and infectivity at the early stages of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/39723/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALMapping Alterations Induced .pdfMapping Alterations Induced .pdfapplication/pdf15523213https://www.arca.fiocruz.br/bitstream/icict/39723/2/Mapping%20Alterations%20Induced%20.pdfa485f8b44283ace87e344a06f1a2edeaMD52TEXTMapping Alterations Induced .pdf.txtMapping Alterations Induced .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach
title Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach
spellingShingle Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach
Crepaldi, Frederico
L. amazonensis
L. amazonensis
Attenuation
Inflammation
Metabolic pathways
Metacyclogenesis
Multiplatform metabolomic fingerprint
title_short Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach
title_full Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach
title_fullStr Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach
title_full_unstemmed Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach
title_sort Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach
author Crepaldi, Frederico
author_facet Crepaldi, Frederico
Toledo, Juliano Simões de
armo, Anderson Oliveira do C.
Machado, Leopoldo Ferreira Marques
Brito, Daniela Diniz Viana de
Serufo, Angela Vieira
Almeida, Ana Paula Martins
Oliveira, Leandro Gonzaga de
Ricotta, Tiago Queiroga Nery
Moreira, Douglas de Souza
Murta, Silvane Maria Fonseca
Diniz, Ariane Barros
Menezes, Gustavo Batista
López-Gonzálvez, Ángeles
Barbas, Coral
Fernandes, Ana Paula
author_role author
author2 Toledo, Juliano Simões de
armo, Anderson Oliveira do C.
Machado, Leopoldo Ferreira Marques
Brito, Daniela Diniz Viana de
Serufo, Angela Vieira
Almeida, Ana Paula Martins
Oliveira, Leandro Gonzaga de
Ricotta, Tiago Queiroga Nery
Moreira, Douglas de Souza
Murta, Silvane Maria Fonseca
Diniz, Ariane Barros
Menezes, Gustavo Batista
López-Gonzálvez, Ángeles
Barbas, Coral
Fernandes, Ana Paula
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Crepaldi, Frederico
Toledo, Juliano Simões de
armo, Anderson Oliveira do C.
Machado, Leopoldo Ferreira Marques
Brito, Daniela Diniz Viana de
Serufo, Angela Vieira
Almeida, Ana Paula Martins
Oliveira, Leandro Gonzaga de
Ricotta, Tiago Queiroga Nery
Moreira, Douglas de Souza
Murta, Silvane Maria Fonseca
Diniz, Ariane Barros
Menezes, Gustavo Batista
López-Gonzálvez, Ángeles
Barbas, Coral
Fernandes, Ana Paula
dc.subject.other.pt_BR.fl_str_mv L. amazonensis
topic L. amazonensis
L. amazonensis
Attenuation
Inflammation
Metabolic pathways
Metacyclogenesis
Multiplatform metabolomic fingerprint
dc.subject.en.pt_BR.fl_str_mv L. amazonensis
Attenuation
Inflammation
Metabolic pathways
Metacyclogenesis
Multiplatform metabolomic fingerprint
description Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil / Centro de Metabolómica y Bioanálisis. Unidad Metabolómica. Interacciones y Bioanálisis (UMIB), Universidad CEU. San Pablo, Boadilla del Monte, Spain.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-02-04T17:13:46Z
dc.date.available.fl_str_mv 2020-02-04T17:13:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CREPALDI, Frederico et al. Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-22, 4 Dec. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39723
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2019.00403
identifier_str_mv CREPALDI, Frederico et al. Mapping Alterations Induced by Long-Term Axenic Cultivation of Leishmania amazonensis Promastigotes With a Multiplatform Metabolomic Fingerprint Approach. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-22, 4 Dec. 2019.
2235-2988
10.3389/fcimb.2019.00403
url https://www.arca.fiocruz.br/handle/icict/39723
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/39723/1/license.txt
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