Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40529 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. |
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Vieira, Paloma de CarvalhoWaghabi, Mariana CaldasBeghini, Daniela GoisPredes, DaniloAbreu, Jose GarciaMouly, VincentButler-Browne, GillianBarbosa, Helene SantosAdesse, Daniel2020-03-26T20:43:59Z2020-03-26T20:43:59Z2019VIEIRA, Paloma de Carvalho et al. Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile. Frontiers in Cellular and Infection Microbiology, v. 9, p.1-16, Nov. 2019.2235-2988https://www.arca.fiocruz.br/handle/icict/4052910.3389/fcimb.2019.00395engFrontiers MediaToxoplasma gondiiMiogêneseToxoplasmose CongênitaFator regulatório miogênicoCélulas C2C12Fibras Musculares EsqueléticasToxoplasma gondiiMyogenesisC2C12 cellsMyotubeMyogenic regulatory factorCongenital toxoplasmosisDesenvolvimento MuscularFibras Musculares EsqueléticasToxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profileinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovação em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Embriologia de Vertebrados. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Embriologia de Vertebrados. Rio de Janeiro, RJ, Brasil.Sorbonne Université. INSERM, Institut de Myologie, Myology Research Center. UMRS974, Paris, France.Sorbonne Université. INSERM, Institut de Myologie, Myology Research Center. UMRS974, Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Toxoplasma gondii is the causative agent of toxoplasmosis, a parasitic disease with a wide global prevalence. The parasite forms cysts in skeletal muscle cells and neurons, although no evident association with inflammatory infiltrates has been typically found. We studied the impact of T. gondii infection on the myogenic program of mouse skeletal muscle cells (SkMC). The C2C12 murine myoblast cell line was infected with T. gondii tachyzoites (ME49 strain) for 24 h followed by myogenic differentiation induction. T. gondii infection caused a general decrease in myotube differentiation, fusion and maturation, along with decreased expression of myosin heavy chain. The expression of Myogenic Regulatory Factors Myf5, MyoD, Mrf4 and myogenin was modulated by the infection. Infected cultures presented increased proliferation rates, as assessed by Ki67 immunostaining, whereas neither host cell lysis nor apoptosis were significantly augmented in infected dishes. Cytokine Bead Array indicated that IL-6 and MCP-1 were highly increased in the medium from infected cultures, whereas TGF-β1 was consistently decreased. Inhibition of the IL-6 receptor or supplementation with recombinant TGF-β failed to reverse the deleterious effects caused by the infection. However, conditioned medium from infected cultures inhibited myogenesis in C2C12 cells. Activation of the Wnt/β-catenin pathway was impaired in T. gondii-infected cultures. Our data indicate that T. gondii leads SkMCs to a pro-inflammatory phenotype, leaving cells unresponsive to β-catenin activation, and inhibition of the myogenic differentiation program. Such deregulation may suggest muscle atrophy and molecular mechanisms similar to those involved in myositis observed in human patients.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40529/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHeleneSBarbosa_PCVieira_etal-IOC_2019.pdfHeleneSBarbosa_PCVieira_etal-IOC_2019.pdfapplication/pdf2526535https://www.arca.fiocruz.br/bitstream/icict/40529/2/HeleneSBarbosa_PCVieira_etal-IOC_2019.pdf4801e366dc3bc1fcea874e83869d4711MD52TEXTHeleneSBarbosa_PCVieira_etal-IOC_2019.pdf.txtHeleneSBarbosa_PCVieira_etal-IOC_2019.pdf.txtExtracted texttext/plain69531https://www.arca.fiocruz.br/bitstream/icict/40529/3/HeleneSBarbosa_PCVieira_etal-IOC_2019.pdf.txtbb946693090222dee34572f0adf03070MD53icict/405292020-03-27 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| dc.title.pt_BR.fl_str_mv |
Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile |
| title |
Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile |
| spellingShingle |
Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile Vieira, Paloma de Carvalho Toxoplasma gondii Miogênese Toxoplasmose Congênita Fator regulatório miogênico Células C2C12 Fibras Musculares Esqueléticas Toxoplasma gondii Myogenesis C2C12 cells Myotube Myogenic regulatory factor Congenital toxoplasmosis Desenvolvimento Muscular Fibras Musculares Esqueléticas |
| title_short |
Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile |
| title_full |
Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile |
| title_fullStr |
Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile |
| title_full_unstemmed |
Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile |
| title_sort |
Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile |
| author |
Vieira, Paloma de Carvalho |
| author_facet |
Vieira, Paloma de Carvalho Waghabi, Mariana Caldas Beghini, Daniela Gois Predes, Danilo Abreu, Jose Garcia Mouly, Vincent Butler-Browne, Gillian Barbosa, Helene Santos Adesse, Daniel |
| author_role |
author |
| author2 |
Waghabi, Mariana Caldas Beghini, Daniela Gois Predes, Danilo Abreu, Jose Garcia Mouly, Vincent Butler-Browne, Gillian Barbosa, Helene Santos Adesse, Daniel |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vieira, Paloma de Carvalho Waghabi, Mariana Caldas Beghini, Daniela Gois Predes, Danilo Abreu, Jose Garcia Mouly, Vincent Butler-Browne, Gillian Barbosa, Helene Santos Adesse, Daniel |
| dc.subject.other.pt_BR.fl_str_mv |
Toxoplasma gondii Miogênese Toxoplasmose Congênita Fator regulatório miogênico Células C2C12 Fibras Musculares Esqueléticas |
| topic |
Toxoplasma gondii Miogênese Toxoplasmose Congênita Fator regulatório miogênico Células C2C12 Fibras Musculares Esqueléticas Toxoplasma gondii Myogenesis C2C12 cells Myotube Myogenic regulatory factor Congenital toxoplasmosis Desenvolvimento Muscular Fibras Musculares Esqueléticas |
| dc.subject.en.pt_BR.fl_str_mv |
Toxoplasma gondii Myogenesis C2C12 cells Myotube Myogenic regulatory factor Congenital toxoplasmosis |
| dc.subject.decs.pt_BR.fl_str_mv |
Desenvolvimento Muscular Fibras Musculares Esqueléticas |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. |
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2019 |
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2019 |
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2020-03-26T20:43:59Z |
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2020-03-26T20:43:59Z |
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info:eu-repo/semantics/article |
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VIEIRA, Paloma de Carvalho et al. Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile. Frontiers in Cellular and Infection Microbiology, v. 9, p.1-16, Nov. 2019. |
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https://www.arca.fiocruz.br/handle/icict/40529 |
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2235-2988 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fcimb.2019.00395 |
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VIEIRA, Paloma de Carvalho et al. Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile. Frontiers in Cellular and Infection Microbiology, v. 9, p.1-16, Nov. 2019. 2235-2988 10.3389/fcimb.2019.00395 |
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Frontiers Media |
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