DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil

Detalhes bibliográficos
Autor(a) principal: Pinto, Israel de Souza
Data de Publicação: 2015
Outros Autores: Chagas, Bruna Dias das, Rodrigues, Andressa Alencastre Fuzari, Ferreira, Adelson Luiz, Rezende, Helder Ricas, Bruno, Rafaela Vieira, Falqueto, Aloisio, Andrade-Filho, José Dilermando, Galati, Eunice Aparecida Bianchi, Shimabukuro, Paloma Helena Fernandes, Brazil, Reginaldo Peçanha, Peixoto, Alexandre Afranio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12411
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil/Universidade Federal do Espírito Santo. Unidade de Medicina Tropical. Vitória, ES, Brasil
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spelling Pinto, Israel de SouzaChagas, Bruna Dias dasRodrigues, Andressa Alencastre FuzariFerreira, Adelson LuizRezende, Helder RicasBruno, Rafaela VieiraFalqueto, AloisioAndrade-Filho, José DilermandoGalati, Eunice Aparecida BianchiShimabukuro, Paloma Helena FernandesBrazil, Reginaldo PeçanhaPeixoto, Alexandre Afranio2015-12-15T12:55:50Z2015-12-15T12:55:50Z2015PINTO, Israel de Souza et al. DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil. PLoS One, vol. 10, n. 10, p. e0140636, 20151932-6203https://www.arca.fiocruz.br/handle/icict/1241110.1371/journal.pone.0140636. eCollection 2015.engPublic Library of ScienceDNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil/Universidade Federal do Espírito Santo. Unidade de Medicina Tropical. Vitória, ES, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, BrasilUniversidade Federal do Espírito Santo. Unidade de Medicina Tropical. Vitória, ES, BrasilUniversidade Federal do Espírito Santo. Unidade de Medicina Tropical. Vitória, ES, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil/Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, BrasilUniversidade Federal do Espírito Santo. Unidade de Medicina Tropical. Vitória, ES, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrasilUniversidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, BrasilDNA barcoding has been an effective tool for species identification in several animal groups. Here, we used DNA barcoding to discriminate between 47 morphologically distinct species of Brazilian sand flies. DNA barcodes correctly identified approximately 90% of the sampled taxa (42 morphologically distinct species) using clustering based on neighbor-joining distance, of which four species showed comparatively higher maximum values of divergence (range 4.23-19.04%), indicating cryptic diversity. The DNA barcodes also corroborated the resurrection of two species within the shannoni complex and provided an efficient tool to differentiate between morphologically indistinguishable females of closely related species. Taken together, our results validate the effectiveness of DNA barcoding for species identification and the discovery of cryptic diversity in sand flies from Brazil.DNA barcodingsand fliesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12411/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjournal.pone.0140636.pdfjournal.pone.0140636.pdfapplication/pdf1306158https://www.arca.fiocruz.br/bitstream/icict/12411/2/journal.pone.0140636.pdf516c3e9c42e1c6e60929790fbe37dd87MD52TEXTjournal.pone.0140636.pdf.txtjournal.pone.0140636.pdf.txtExtracted texttext/plain69988https://www.arca.fiocruz.br/bitstream/icict/12411/3/journal.pone.0140636.pdf.txtd2d6e4134b2fc0f27ac6b02e17901f30MD53icict/124112019-06-19 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dc.title.pt_BR.fl_str_mv DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
title DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
spellingShingle DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
Pinto, Israel de Souza
DNA barcoding
sand flies
title_short DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
title_full DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
title_fullStr DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
title_full_unstemmed DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
title_sort DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil
author Pinto, Israel de Souza
author_facet Pinto, Israel de Souza
Chagas, Bruna Dias das
Rodrigues, Andressa Alencastre Fuzari
Ferreira, Adelson Luiz
Rezende, Helder Ricas
Bruno, Rafaela Vieira
Falqueto, Aloisio
Andrade-Filho, José Dilermando
Galati, Eunice Aparecida Bianchi
Shimabukuro, Paloma Helena Fernandes
Brazil, Reginaldo Peçanha
Peixoto, Alexandre Afranio
author_role author
author2 Chagas, Bruna Dias das
Rodrigues, Andressa Alencastre Fuzari
Ferreira, Adelson Luiz
Rezende, Helder Ricas
Bruno, Rafaela Vieira
Falqueto, Aloisio
Andrade-Filho, José Dilermando
Galati, Eunice Aparecida Bianchi
Shimabukuro, Paloma Helena Fernandes
Brazil, Reginaldo Peçanha
Peixoto, Alexandre Afranio
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinto, Israel de Souza
Chagas, Bruna Dias das
Rodrigues, Andressa Alencastre Fuzari
Ferreira, Adelson Luiz
Rezende, Helder Ricas
Bruno, Rafaela Vieira
Falqueto, Aloisio
Andrade-Filho, José Dilermando
Galati, Eunice Aparecida Bianchi
Shimabukuro, Paloma Helena Fernandes
Brazil, Reginaldo Peçanha
Peixoto, Alexandre Afranio
dc.subject.en.pt_BR.fl_str_mv DNA barcoding
sand flies
topic DNA barcoding
sand flies
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil/Universidade Federal do Espírito Santo. Unidade de Medicina Tropical. Vitória, ES, Brasil
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-12-15T12:55:50Z
dc.date.available.fl_str_mv 2015-12-15T12:55:50Z
dc.date.issued.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PINTO, Israel de Souza et al. DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil. PLoS One, vol. 10, n. 10, p. e0140636, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12411
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0140636. eCollection 2015.
identifier_str_mv PINTO, Israel de Souza et al. DNA Barcoding of Neotropical Sand Flies (Diptera, Psychodidae, Phlebotominae): Species Identification and Discovery within Brazil. PLoS One, vol. 10, n. 10, p. e0140636, 2015
1932-6203
10.1371/journal.pone.0140636. eCollection 2015.
url https://www.arca.fiocruz.br/handle/icict/12411
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12411/1/license.txt
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