Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/34647 |
Resumo: | Inclui correção. |
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Mata, Verónica ElizabethPassos, Sonia Regina LambertHökerberg, Yara Hahr MarquesBerardinelli, Guilherme MiguéisSantos, Maria Angelica Borges dosFukuoka, Levy Vilas BoasMaciel, Anna Carolina Fontoura Seixas RangelRodrigues, Cintia Damasceno dos SantosSantos, Aline da SilvaOliveira, Raquel de Vasconcellos Carvalhaes de2019-08-07T13:25:15Z2019-08-07T13:25:15Z2017MATA, Verónica Elizabeth et al. Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory. BMC Infectious Diseases, v. 17, p. 1-7, 2017.1471-2334https://www.arca.fiocruz.br/handle/icict/3464710.1186/s12879-017-2679-z1471-2334Inclui correção.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Sem afiliação.Sem afiliação.Sem afiliação.Sem afiliação.Sem afiliação.Sem afiliação.Sem afiliação.Sem afiliação.Sem afiliação.Background: Rapid immunochromatographic tests (ICT) for dengue non-structural protein 1 (NS1) have shown good performance for diagnosing acute-phase dengue in serum in laboratory settings, but rarely have been assessed in whole blood and at point of care (POC). This study compare the accuracy and inter- and intra-observer reliability of the NS1 Bioeasy™ ICT in whole blood at POC versus serum in the laboratory, during a DENV-1 epidemic. Methods: Cross-sectional study involving 144 adults spontaneously demanding care in an emergency department within 4 days of onset of acute febrile illness. Accuracy of NS1 Bioeasy™ ICT was compared in whole blood and serum, both at 15 and 30 min, blinded to the reference RT-PCR or NS1 ELISA. Non-dengue patients were also tested for Zika virus with RT-PCR. Reliability of whole blood and serum readings by the same or different observers was measured by simple kappa (95% CI). Results: At 15 min, sensitivity (Sn) of NS1 Bioeasy™ ICT in whole blood/POC was 76.7% (95% CI: 68.0–84.1) and specificity (Sp) was 87.0% (95% CI: 66.4–97.2). Sn in serum/laboratory was 82% (95% CI: 74.1–88.6) and Sp 100% (95% CI: 85.8–100). Positive likelihood ratio was 5.9 (95% CI: 2.0–17.0) for whole blood/POC and 19.8 (95% CI: 2.9–135.1) for serum/laboratory. Reliability of matched readings of whole blood/POC and serum/laboratory by the same observer (k = 0.83, 95% CI: 0.74–0.93) or different observers (k = 0.81, 95% CI: 0.72–0.92) was almost perfect, with higher discordant levels in the absence of dengue. Results did not differ statistically at 5%. Conclusions: NS1 Bioeasy™ ICT in DENV-1 epidemics is a potentially confirmatory test. Invalid results at 15 min should be reread at 30 min. To optimize impact of implementing ICT in the management of false-negatives it should be incorporated into an algorithm according to setting and available specimen.engBMCAccuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratoryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDengueSensitivity and specificityDiagnosisPoint-of-care systemsNS1Reproducibility of resultsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory |
| title |
Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory |
| spellingShingle |
Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory Mata, Verónica Elizabeth Dengue Sensitivity and specificity Diagnosis Point-of-care systems NS1 Reproducibility of results |
| title_short |
Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory |
| title_full |
Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory |
| title_fullStr |
Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory |
| title_full_unstemmed |
Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory |
| title_sort |
Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory |
| author |
Mata, Verónica Elizabeth |
| author_facet |
Mata, Verónica Elizabeth Passos, Sonia Regina Lambert Hökerberg, Yara Hahr Marques Berardinelli, Guilherme Miguéis Santos, Maria Angelica Borges dos Fukuoka, Levy Vilas Boas Maciel, Anna Carolina Fontoura Seixas Rangel Rodrigues, Cintia Damasceno dos Santos Santos, Aline da Silva Oliveira, Raquel de Vasconcellos Carvalhaes de |
| author_role |
author |
| author2 |
Passos, Sonia Regina Lambert Hökerberg, Yara Hahr Marques Berardinelli, Guilherme Miguéis Santos, Maria Angelica Borges dos Fukuoka, Levy Vilas Boas Maciel, Anna Carolina Fontoura Seixas Rangel Rodrigues, Cintia Damasceno dos Santos Santos, Aline da Silva Oliveira, Raquel de Vasconcellos Carvalhaes de |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mata, Verónica Elizabeth Passos, Sonia Regina Lambert Hökerberg, Yara Hahr Marques Berardinelli, Guilherme Miguéis Santos, Maria Angelica Borges dos Fukuoka, Levy Vilas Boas Maciel, Anna Carolina Fontoura Seixas Rangel Rodrigues, Cintia Damasceno dos Santos Santos, Aline da Silva Oliveira, Raquel de Vasconcellos Carvalhaes de |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue Sensitivity and specificity Diagnosis Point-of-care systems NS1 Reproducibility of results |
| topic |
Dengue Sensitivity and specificity Diagnosis Point-of-care systems NS1 Reproducibility of results |
| description |
Inclui correção. |
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2017 |
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2017 |
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2019-08-07T13:25:15Z |
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2019-08-07T13:25:15Z |
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MATA, Verónica Elizabeth et al. Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory. BMC Infectious Diseases, v. 17, p. 1-7, 2017. |
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https://www.arca.fiocruz.br/handle/icict/34647 |
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1471-2334 |
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10.1186/s12879-017-2679-z |
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1471-2334 |
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MATA, Verónica Elizabeth et al. Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory. BMC Infectious Diseases, v. 17, p. 1-7, 2017. 1471-2334 10.1186/s12879-017-2679-z |
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