The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro

Detalhes bibliográficos
Autor(a) principal: Oliveira, Laíse Brandão
Data de Publicação: 2019
Outros Autores: Celes, Fabiana Santana, Paiva, Claudia Neto, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34711
Resumo: CNPq fellowship (Iniciação Científica). FC was financed by the Coordenação de Aperfeiçoamento de pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. CO is a senior investigator from CNPq. This work was funded in part by Instituto Gonçalo Moniz (IGM)-Fiocruz.
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spelling Oliveira, Laíse BrandãoCeles, Fabiana SantanaPaiva, Claudia NetoOliveira, Camila Indiani de2019-08-12T12:45:52Z2019-08-12T12:45:52Z2019OLIVEIRA, Laíse Brandão et al. The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-7, 2019.2235-2988https://www.arca.fiocruz.br/handle/icict/3471110.3389/fcimb.2019.00237CNPq fellowship (Iniciação Científica). FC was financed by the Coordenação de Aperfeiçoamento de pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. CO is a senior investigator from CNPq. This work was funded in part by Instituto Gonçalo Moniz (IGM)-Fiocruz.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil.Leishmaniasis is an infectious disease caused by protozoans of the genus Leishmania. The macrophage is the resident cell in which the parasite replicates and it is important to identify new compounds that can aid in parasite elimination since the drugs used to treat leishmaniasis are toxic and present side effects. We have previously shown that treatment of Leishmania braziliensis-infected macrophages with DETC (Diethyldithiocarbamate) induces parasite killing, in vivo. Thus, the objective of this study was to further evaluate the effect of oxidants and antioxidants in L. braziliensis-infected macrophages, following treatment with either oxidizing Hydrogen Peroxide, Menadione, DETC, or antioxidant [NAC (N-Acetyl-Cyteine), Apocynin, and Tempol] compounds. We determined the percentage of infected macrophages and number of amastigotes. Promastigote survival was also evaluated. Both DETC (SOD-inhibitor) and Tempol (SOD-mimetic) decreased the percentage of infected cells and parasite load. Hydrogen peroxide did not interfere with parasite burden, while superoxide-generator Menadione had a reducing effect. On the other hand, NAC (GSH-replenisher) and Apocynin (NADPH-oxidase inhibitor) increased parasite burden. Tempol surfaces as an interesting candidate for the chemotherapy of CL with an IC50 of 0.66 ± 0.08 mM and selectivity index of 151. While it remains obscure how a SOD-mimetic may induce leishmanicidal effects, we suggest the possibility of developing Tempol-based topical applications for the treatment of cutaneous leishmaniasis caused by L. braziliensis.engFrontiers MediaL. braziliensisOxidantesAnti-oxidantesLeishmanioseQuimioterapiaL. braziliensisOxidantsAnti-oxidantsLeishmaniasisChemotherapyThe Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34711/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALOliveira, L.B The paradoxical.pdfOliveira, L.B The 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dc.title.pt_BR.fl_str_mv The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro
title The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro
spellingShingle The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro
Oliveira, Laíse Brandão
L. braziliensis
Oxidantes
Anti-oxidantes
Leishmaniose
Quimioterapia
L. braziliensis
Oxidants
Anti-oxidants
Leishmaniasis
Chemotherapy
title_short The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro
title_full The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro
title_fullStr The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro
title_full_unstemmed The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro
title_sort The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro
author Oliveira, Laíse Brandão
author_facet Oliveira, Laíse Brandão
Celes, Fabiana Santana
Paiva, Claudia Neto
Oliveira, Camila Indiani de
author_role author
author2 Celes, Fabiana Santana
Paiva, Claudia Neto
Oliveira, Camila Indiani de
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Laíse Brandão
Celes, Fabiana Santana
Paiva, Claudia Neto
Oliveira, Camila Indiani de
dc.subject.other.pt_BR.fl_str_mv L. braziliensis
Oxidantes
Anti-oxidantes
Leishmaniose
Quimioterapia
topic L. braziliensis
Oxidantes
Anti-oxidantes
Leishmaniose
Quimioterapia
L. braziliensis
Oxidants
Anti-oxidants
Leishmaniasis
Chemotherapy
dc.subject.en.pt_BR.fl_str_mv L. braziliensis
Oxidants
Anti-oxidants
Leishmaniasis
Chemotherapy
description CNPq fellowship (Iniciação Científica). FC was financed by the Coordenação de Aperfeiçoamento de pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. CO is a senior investigator from CNPq. This work was funded in part by Instituto Gonçalo Moniz (IGM)-Fiocruz.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-12T12:45:52Z
dc.date.available.fl_str_mv 2019-08-12T12:45:52Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Laíse Brandão et al. The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-7, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34711
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2019.00237
identifier_str_mv OLIVEIRA, Laíse Brandão et al. The Paradoxical Leishmanicidal Effects of Superoxide Dismutase (SOD)-Mimetic Tempol in Leishmania braziliensis Infection in vitro. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-7, 2019.
2235-2988
10.3389/fcimb.2019.00237
url https://www.arca.fiocruz.br/handle/icict/34711
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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