Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers

Detalhes bibliográficos
Autor(a) principal: Pompeu, Margarida Maria de Lima
Data de Publicação: 2001
Outros Autores: Brodskyn, Claudia Ida, Teixeira, M. J., Clarêncio, Jorge, Van Weyenbergh, Johan Jozef Rosa Maria, Coelho, I. C, Cardoso, Silvia Andrade, Barral, Aldina Maria Prado, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7441
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
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spelling Pompeu, Margarida Maria de LimaBrodskyn, Claudia IdaTeixeira, M. J.Clarêncio, JorgeVan Weyenbergh, Johan Jozef Rosa MariaCoelho, I. CCardoso, Silvia AndradeBarral, Aldina Maria PradoBarral Netto, Manoel2014-03-24T18:35:36Z2014-03-24T18:35:36Z2001POMPEU, M. M. et al. Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers. Infection and Immunity, v. 69, n. 12, p. 7453-7460, 2001.0019-9567https://www.arca.fiocruz.br/handle/icict/744110.1128/IAI.69.12.7453–7460.2001engAmerican Society for Microbiology.Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Universidade Federal do Ceará. Núcleo de Medicina Tropical. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Ceará. Núcleo de Medicina Tropical. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.The initial encounter of Leishmania cells and cells from the immune system is fundamentally important in the outcome of infection and determines disease development or resistance. We evaluated the anti-Leishmania amazonensis response of naive volunteers by using an in vitro priming (IVP) system and comparing the responses following in vivo vaccination against the same parasite. In vitro stimulation allowed us to distinguish two groups of individuals, those who produced small amounts of gamma interferon (IFN-gamma) (n = 16) (low producers) and those who produced large amounts of this cytokine (n = 16) (high producers). IFN-gamma production was proportional to tumor necrosis factor alpha and interleukin 10 (IL-10) levels but did not correlate with IL-5 production. Volunteers who produced small amounts of IFN-gamma in vitro remained low producers 40 days after vaccination, whereas high producers exhibited increased IFN-gamma production. However, 6 months after vaccination, all individuals tested produced similarly high levels of IFN-gamma upon stimulation of their peripheral blood mononuclear cells with Leishmania promastigotes, indicating that low in vitro producers respond slowly in vivo to vaccination. In high IFN-gamma producers there was an increased frequency of activated CD8(+) T cells both in vitro and in vivo compared to the frequency in low producers, and such cells were positive for IFN-gamma as determined by intracellular staining. Such findings suggest that IVP responses can be used to predict the pace of postvaccination responses of test volunteers. Although all vaccinated individuals eventually have a potent anti-Leishmania cell-mediated immunity (CMI) response, a delay in mounting the CMI response may influence resistance against leishmaniasis.Interferon gama/biossínteseLeishmaniose/imunologiaVacinas Protozoárias/imunologiaAdolescenteAdultoLinfócitos T CD8-Positivos/imunologiaPrevisõesHumanosImunidade InataAtivação LinfocitáriaMasculinoReceptores de Interleucina-2/isolamento & purificaçãoSubpopulações de Linfócitos T/imunologiaVacinaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPompeu M M L Differences in gamma....pdfPompeu M M L Differences in gamma....pdfapplication/pdf176082https://www.arca.fiocruz.br/bitstream/icict/7441/1/Pompeu%20M%20M%20L%20Differences%20in%20gamma....pdf22a1cde774c856914d4036413f376369MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7441/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTPompeu M M L Differences in gamma....pdf.txtPompeu M M L Differences in gamma....pdf.txtExtracted texttext/plain40586https://www.arca.fiocruz.br/bitstream/icict/7441/5/Pompeu%20M%20M%20L%20Differences%20in%20gamma....pdf.txtc0e79e3a3d834419f295bcd995d68db5MD55THUMBNAILPompeu M M L Differences in gamma....pdf.jpgPompeu M M L Differences in gamma....pdf.jpgGenerated Thumbnailimage/jpeg2350https://www.arca.fiocruz.br/bitstream/icict/7441/4/Pompeu%20M%20M%20L%20Differences%20in%20gamma....pdf.jpg22e13c3f94e6eb843a2aa791009e5bb5MD54icict/74412023-03-15 14:34:19.526oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers
title Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers
spellingShingle Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers
Pompeu, Margarida Maria de Lima
Interferon gama/biossíntese
Leishmaniose/imunologia
Vacinas Protozoárias/imunologia
Adolescente
Adulto
Linfócitos T CD8-Positivos/imunologia
Previsões
Humanos
Imunidade Inata
Ativação Linfocitária
Masculino
Receptores de Interleucina-2/isolamento & purificação
Subpopulações de Linfócitos T/imunologia
Vacinação
title_short Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers
title_full Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers
title_fullStr Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers
title_full_unstemmed Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers
title_sort Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers
author Pompeu, Margarida Maria de Lima
author_facet Pompeu, Margarida Maria de Lima
Brodskyn, Claudia Ida
Teixeira, M. J.
Clarêncio, Jorge
Van Weyenbergh, Johan Jozef Rosa Maria
Coelho, I. C
Cardoso, Silvia Andrade
Barral, Aldina Maria Prado
Barral Netto, Manoel
author_role author
author2 Brodskyn, Claudia Ida
Teixeira, M. J.
Clarêncio, Jorge
Van Weyenbergh, Johan Jozef Rosa Maria
Coelho, I. C
Cardoso, Silvia Andrade
Barral, Aldina Maria Prado
Barral Netto, Manoel
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pompeu, Margarida Maria de Lima
Brodskyn, Claudia Ida
Teixeira, M. J.
Clarêncio, Jorge
Van Weyenbergh, Johan Jozef Rosa Maria
Coelho, I. C
Cardoso, Silvia Andrade
Barral, Aldina Maria Prado
Barral Netto, Manoel
dc.subject.decs.pt_BR.fl_str_mv Interferon gama/biossíntese
Leishmaniose/imunologia
Vacinas Protozoárias/imunologia
Adolescente
Adulto
Linfócitos T CD8-Positivos/imunologia
Previsões
Humanos
Imunidade Inata
Ativação Linfocitária
Masculino
Receptores de Interleucina-2/isolamento & purificação
Subpopulações de Linfócitos T/imunologia
Vacinação
topic Interferon gama/biossíntese
Leishmaniose/imunologia
Vacinas Protozoárias/imunologia
Adolescente
Adulto
Linfócitos T CD8-Positivos/imunologia
Previsões
Humanos
Imunidade Inata
Ativação Linfocitária
Masculino
Receptores de Interleucina-2/isolamento & purificação
Subpopulações de Linfócitos T/imunologia
Vacinação
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2014-03-24T18:35:36Z
dc.date.available.fl_str_mv 2014-03-24T18:35:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv POMPEU, M. M. et al. Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers. Infection and Immunity, v. 69, n. 12, p. 7453-7460, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7441
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.69.12.7453–7460.2001
identifier_str_mv POMPEU, M. M. et al. Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers. Infection and Immunity, v. 69, n. 12, p. 7453-7460, 2001.
0019-9567
10.1128/IAI.69.12.7453–7460.2001
url https://www.arca.fiocruz.br/handle/icict/7441
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology.
publisher.none.fl_str_mv American Society for Microbiology.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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