The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41209 |
Resumo: | Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. |
| id |
CRUZ_7d09bec340eda46d4baeaeb9d4187fb9 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/41209 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Fracaro, LetíciaSenegaglia, Alexandra C.Herai, Roberto H.Leitolis, AmandaBoldrini-Leite, Lidiane M.Rebelatto, Carmen L. K.Travers, Paul J.Brofman, Paulo R SDominguez, Alejandro Correa2020-05-12T19:32:40Z2020-05-12T19:32:40Z2020FRACARO, Leticia et al. The Expression Profile of Dental Pulp-Derived Stromal Cells Supports Their Limited Capacity to Differentiate into Adipogenic Cells. International Journal of Molecular Sciences. V.21, n.2753, p. 1-17, 2020.1422-0067https://www.arca.fiocruz.br/handle/icict/4120910.3390/ijms21082753porMDPIDPSCsADSCsMesenchymal Stem CellsDental PulpAdipose TissueAdipogenesisTranscription FactorsWnt Signaling PathwayCélulas Madre MesenquimatosasPulpa DentalTejido AdiposoAdipogénesisFactores de TranscripciónVía de Señalización WntCélulas-Tronco MesenquimaisPolpa DentáriaTecido AdiposoAdipogeniaFatores de TranscriçãoVia de Sinalização WntThe expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Centre for Regenerative Medicine. University of Edinburgh. Edinburgh, Scotland.Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Mesenchymal stromal cells (MSCs) can self-renew, differentiate into specialised cells and have different embryonic origins-ectodermal for dental pulp-derived MSCs (DPSCs) and mesodermal for adipose tissue-derived MSCs (ADSCs). Data on DPSCs adipogenic differentiation potential and timing vary, and the lack of molecular and genetic information prompted us to gain a better understanding of DPSCs adipogenic differentiation potential and gene expression profile. While DPSCs differentiated readily along osteogenic and chondrogenic pathways, after 21 days in two different types of adipogenic induction media, DPSCs cultures did not contain lipid vacuoles and had low expression levels of the adipogenic genes proliferator-activated receptor gamma (PPARG), lipoprotein lipase (LPL) and CCAAT/enhancer-binding protein alpha (CEBPA). To better understand this limitation in adipogenesis, transcriptome analysis in undifferentiated DPSCs was carried out, with the ADSC transcriptome used as a positive control. In total, 14,871 transcripts were common to DPSCs and ADSCs, some were unique (DPSCs: 471, ADSCs: 1032), and 510 were differentially expressed genes. Detailed analyses of overrepresented transcripts showed that DPSCs express genes that inhibit adipogenic differentiation, revealing the possible mechanism for their limited adipogenesis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/41209/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALijms-21-02753-v.pdfijms-21-02753-v.pdfapplication/pdf2890264https://www.arca.fiocruz.br/bitstream/icict/41209/2/ijms-21-02753-v.pdfa68865595f30826a5737811896063df8MD52TEXTijms-21-02753-v.pdf.txtijms-21-02753-v.pdf.txtExtracted texttext/plain63356https://www.arca.fiocruz.br/bitstream/icict/41209/3/ijms-21-02753-v.pdf.txt9bb79502206119679a74c062522a00d8MD53icict/412092020-05-13 02:07:09.284oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-05-13T05:07:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells |
| title |
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells |
| spellingShingle |
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells Fracaro, Letícia DPSCs ADSCs Mesenchymal Stem Cells Dental Pulp Adipose Tissue Adipogenesis Transcription Factors Wnt Signaling Pathway Células Madre Mesenquimatosas Pulpa Dental Tejido Adiposo Adipogénesis Factores de Transcripción Vía de Señalización Wnt Células-Tronco Mesenquimais Polpa Dentária Tecido Adiposo Adipogenia Fatores de Transcrição Via de Sinalização Wnt |
| title_short |
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells |
| title_full |
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells |
| title_fullStr |
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells |
| title_full_unstemmed |
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells |
| title_sort |
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells |
| author |
Fracaro, Letícia |
| author_facet |
Fracaro, Letícia Senegaglia, Alexandra C. Herai, Roberto H. Leitolis, Amanda Boldrini-Leite, Lidiane M. Rebelatto, Carmen L. K. Travers, Paul J. Brofman, Paulo R S Dominguez, Alejandro Correa |
| author_role |
author |
| author2 |
Senegaglia, Alexandra C. Herai, Roberto H. Leitolis, Amanda Boldrini-Leite, Lidiane M. Rebelatto, Carmen L. K. Travers, Paul J. Brofman, Paulo R S Dominguez, Alejandro Correa |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fracaro, Letícia Senegaglia, Alexandra C. Herai, Roberto H. Leitolis, Amanda Boldrini-Leite, Lidiane M. Rebelatto, Carmen L. K. Travers, Paul J. Brofman, Paulo R S Dominguez, Alejandro Correa |
| dc.subject.other.pt_BR.fl_str_mv |
DPSCs ADSCs |
| topic |
DPSCs ADSCs Mesenchymal Stem Cells Dental Pulp Adipose Tissue Adipogenesis Transcription Factors Wnt Signaling Pathway Células Madre Mesenquimatosas Pulpa Dental Tejido Adiposo Adipogénesis Factores de Transcripción Vía de Señalización Wnt Células-Tronco Mesenquimais Polpa Dentária Tecido Adiposo Adipogenia Fatores de Transcrição Via de Sinalização Wnt |
| dc.subject.en.pt_BR.fl_str_mv |
Mesenchymal Stem Cells Dental Pulp Adipose Tissue Adipogenesis Transcription Factors Wnt Signaling Pathway |
| dc.subject.es.pt_BR.fl_str_mv |
Células Madre Mesenquimatosas Pulpa Dental Tejido Adiposo Adipogénesis Factores de Transcripción Vía de Señalización Wnt |
| dc.subject.decs.pt_BR.fl_str_mv |
Células-Tronco Mesenquimais Polpa Dentária Tecido Adiposo Adipogenia Fatores de Transcrição Via de Sinalização Wnt |
| description |
Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. |
| publishDate |
2020 |
| dc.date.accessioned.fl_str_mv |
2020-05-12T19:32:40Z |
| dc.date.available.fl_str_mv |
2020-05-12T19:32:40Z |
| dc.date.issued.fl_str_mv |
2020 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
FRACARO, Leticia et al. The Expression Profile of Dental Pulp-Derived Stromal Cells Supports Their Limited Capacity to Differentiate into Adipogenic Cells. International Journal of Molecular Sciences. V.21, n.2753, p. 1-17, 2020. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/41209 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1422-0067 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/ijms21082753 |
| identifier_str_mv |
FRACARO, Leticia et al. The Expression Profile of Dental Pulp-Derived Stromal Cells Supports Their Limited Capacity to Differentiate into Adipogenic Cells. International Journal of Molecular Sciences. V.21, n.2753, p. 1-17, 2020. 1422-0067 10.3390/ijms21082753 |
| url |
https://www.arca.fiocruz.br/handle/icict/41209 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/41209/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/41209/2/ijms-21-02753-v.pdf https://www.arca.fiocruz.br/bitstream/icict/41209/3/ijms-21-02753-v.pdf.txt |
| bitstream.checksum.fl_str_mv |
783568c2893d2e25a99990b126be1772 a68865595f30826a5737811896063df8 9bb79502206119679a74c062522a00d8 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1798324975278489600 |