Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene

Detalhes bibliográficos
Autor(a) principal: Santos, Eduardo L.
Data de Publicação: 2021
Outros Autores: Freitas, Priscilla R., Araújo, Ana Carolina J., Almeida, Ray S., Tintino, Saulo R., Paulo, Cicera Laura R., Silva, Ana Cristina A., Silva, Luiz E., Amaral, Wanderlei do, Deschamps, Cícero, Siqueira Junior, José Pinto, Barbosa Filho, José Maria, Sousa, Gabriela Ribeiro de, Ribeiro Filho, Jaime, Coutinho, Henrique D. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/48710
Resumo: a Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, Brazil b Maurício de Nassau College -UNINASSAU, Petrolina, PE, Brazil c Post graduate Program in Sustainable Territorial Development, Federal University of Parana ´ – UFPR, Matinhos, Brazil d Post graduate Program in Agronomy, Federal University of Parana ´ - UFPR, Curitiba, Brazil e Department of Molecular Biology, Federal University of Paraiba-UFPB, Joao ˜ Pessoa 58051-900, PB, Brazil f Departmen of Pharmacy, Federal University of Paraiba-UFPB, Joao ˜ Pessoa 58051-900, PB, Brazil g Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, BA, Brazil
id CRUZ_7ec3a9f8aaba6d842a24e9fa23b80f36
oai_identifier_str oai:www.arca.fiocruz.br:icict/48710
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Santos, Eduardo L.Freitas, Priscilla R.Araújo, Ana Carolina J.Almeida, Ray S.Tintino, Saulo R.Paulo, Cicera Laura R.Silva, Ana Cristina A.Silva, Luiz E.Amaral, Wanderlei doDeschamps, CíceroSiqueira Junior, José PintoBarbosa Filho, José MariaSousa, Gabriela Ribeiro deRibeiro Filho, JaimeCoutinho, Henrique D. M.2021-08-24T11:04:58Z2021-08-24T11:04:58Z2021SANTOS, Eduardo L. et al. Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene. Phytomedicine Plus, 2021.2667-0313https://www.arca.fiocruz.br/handle/icict/4871010.1016/j.phyplu.2021.100100a Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, Brazil b Maurício de Nassau College -UNINASSAU, Petrolina, PE, Brazil c Post graduate Program in Sustainable Territorial Development, Federal University of Parana ´ – UFPR, Matinhos, Brazil d Post graduate Program in Agronomy, Federal University of Parana ´ - UFPR, Curitiba, Brazil e Department of Molecular Biology, Federal University of Paraiba-UFPB, Joao ˜ Pessoa 58051-900, PB, Brazil f Departmen of Pharmacy, Federal University of Paraiba-UFPB, Joao ˜ Pessoa 58051-900, PB, Brazil g Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, BA, Brazil“Múltipla – ver em notas”Aloysia gratissima (Verbenaceae), popularly known as bee-brush or whitebrush, has been widely used in Brazilian folk medicine as analgesic, expectorant and antimicrobial. Phytochemical studies have identified β-caryophyllene as one of the major components of the essential oil of this plant. This bioactive sesquiterpene has been shown to have anti-inflammatory and antibacterial activities. Purpose: The present study aimed to characterize the chemical profile and evaluate the antibacterial and antibiotic-enhancing activities of the essential oil obtained from Aloysia gratíssima (EOAG) and β-caryophyllene. Methods: The phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GCMS). The antibacterial activity against Pseudomonas aeruginosa 24, Staphylococcus aureus 10, and Escherichia coli 06 was assessed by determining the minimum inhibitory concentration (MIC) using the broth microdilution method. The potentiation of the antibiotic activity by the EOAG and the β-caryophyllene was performed using the MIC evaluation of the antibiotic alone or in association with the essential oil and its main compound. To evaluate the minimum reduction of the drug concentration necessary, the Dose Reduction Index (DRI) was calculated. Results: A reduction in the MIC of the antibiotics against strains treated simultaneously with the essential oil or β-caryophyllene was observed. The GC-MS analysis of the EOAG identified 30 compounds, including β-caryophyllene as the major component. Both OEAG and β-caryophyllene presented antibacterial activity against S. aureus, in addition to potentiating the action of norfloxacin against S. aureus, P. aeruginosa, and E. coli. Conclusions: These substances also reversed the antibiotic resistance to gentamicin and erythromycin against of S. aureus and P. aeruginosa, respectively.engElsevierVerbenaceaePseudomonas aeruginosaÓleos essenciaisResistência a antibióticosStaphylococcus aureusAloysia gratíssimaβ-caryophylleneEssential oilsAntibiotic resistanceIn vitro studyEnhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophylleneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/48710/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALSantos, Eduardo L. Enhanced....pdfSantos, Eduardo L. Enhanced....pdfapplication/pdf1181752https://www.arca.fiocruz.br/bitstream/icict/48710/2/Santos%2c%20Eduardo%20L.%20Enhanced....pdfa470d218491ef9ec476b9aabe0e2d882MD52TEXTSantos, Eduardo L. Enhanced....pdf.txtSantos, Eduardo L. Enhanced....pdf.txtExtracted texttext/plain39823https://www.arca.fiocruz.br/bitstream/icict/48710/3/Santos%2c%20Eduardo%20L.%20Enhanced....pdf.txt2574adc05231cd5a8b4c04bf95de573fMD53icict/487102023-03-15 14:34:02.471oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:02Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
title Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
spellingShingle Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
Santos, Eduardo L.
Verbenaceae
Pseudomonas aeruginosa
Óleos essenciais
Resistência a antibióticos
Staphylococcus aureus
Aloysia gratíssima
β-caryophyllene
Essential oils
Antibiotic resistance
In vitro study
title_short Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
title_full Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
title_fullStr Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
title_full_unstemmed Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
title_sort Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
author Santos, Eduardo L.
author_facet Santos, Eduardo L.
Freitas, Priscilla R.
Araújo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Silva, Ana Cristina A.
Silva, Luiz E.
Amaral, Wanderlei do
Deschamps, Cícero
Siqueira Junior, José Pinto
Barbosa Filho, José Maria
Sousa, Gabriela Ribeiro de
Ribeiro Filho, Jaime
Coutinho, Henrique D. M.
author_role author
author2 Freitas, Priscilla R.
Araújo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Silva, Ana Cristina A.
Silva, Luiz E.
Amaral, Wanderlei do
Deschamps, Cícero
Siqueira Junior, José Pinto
Barbosa Filho, José Maria
Sousa, Gabriela Ribeiro de
Ribeiro Filho, Jaime
Coutinho, Henrique D. M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Eduardo L.
Freitas, Priscilla R.
Araújo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Silva, Ana Cristina A.
Silva, Luiz E.
Amaral, Wanderlei do
Deschamps, Cícero
Siqueira Junior, José Pinto
Barbosa Filho, José Maria
Sousa, Gabriela Ribeiro de
Ribeiro Filho, Jaime
Coutinho, Henrique D. M.
dc.subject.other.pt_BR.fl_str_mv Verbenaceae
Pseudomonas aeruginosa
Óleos essenciais
Resistência a antibióticos
Staphylococcus aureus
topic Verbenaceae
Pseudomonas aeruginosa
Óleos essenciais
Resistência a antibióticos
Staphylococcus aureus
Aloysia gratíssima
β-caryophyllene
Essential oils
Antibiotic resistance
In vitro study
dc.subject.en.pt_BR.fl_str_mv Aloysia gratíssima
β-caryophyllene
Essential oils
Antibiotic resistance
In vitro study
description a Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, Brazil b Maurício de Nassau College -UNINASSAU, Petrolina, PE, Brazil c Post graduate Program in Sustainable Territorial Development, Federal University of Parana ´ – UFPR, Matinhos, Brazil d Post graduate Program in Agronomy, Federal University of Parana ´ - UFPR, Curitiba, Brazil e Department of Molecular Biology, Federal University of Paraiba-UFPB, Joao ˜ Pessoa 58051-900, PB, Brazil f Departmen of Pharmacy, Federal University of Paraiba-UFPB, Joao ˜ Pessoa 58051-900, PB, Brazil g Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, BA, Brazil
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-08-24T11:04:58Z
dc.date.available.fl_str_mv 2021-08-24T11:04:58Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Eduardo L. et al. Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene. Phytomedicine Plus, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/48710
dc.identifier.issn.pt_BR.fl_str_mv 2667-0313
dc.identifier.eissn.none.fl_str_mv 10.1016/j.phyplu.2021.100100
identifier_str_mv SANTOS, Eduardo L. et al. Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene. Phytomedicine Plus, 2021.
2667-0313
10.1016/j.phyplu.2021.100100
url https://www.arca.fiocruz.br/handle/icict/48710
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/48710/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/48710/2/Santos%2c%20Eduardo%20L.%20Enhanced....pdf
https://www.arca.fiocruz.br/bitstream/icict/48710/3/Santos%2c%20Eduardo%20L.%20Enhanced....pdf.txt
bitstream.checksum.fl_str_mv 36b51ef91c52b5338d9d29ba0cc807bc
a470d218491ef9ec476b9aabe0e2d882
2574adc05231cd5a8b4c04bf95de573f
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324646685179904

Registros relacionados