Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42710 |
Resumo: | Tainá Raiol - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado em mais de uma unidade da Fiocruz, mas não consta à informação no documento do vinculo com a Fiocruz Brasília. |
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Siqueira, Isaque MedeirosCastro, Raffael Júnio Araújo deLeonhardt, Luiza Chaves de MirandaJerônimo, Márcio SousaSoares, Aluízio CarlosRaiol, TaináNishibe, ChristianeAlmeida, NalvoTavares, Aldo HenriqueHoffmann, ChristianBocca, Anamelia Lorenzetti2020-08-12T13:33:52Z2020-08-12T13:33:52Z2017SIQUEIRA, Isaque Medeiros et al. Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity. PloS neglected tropical diseases, [San Francisco], v.11, n.3, p. 1-23, 2017.1935-2727https://www.arca.fiocruz.br/handle/icict/4271010.1371/journal.pntd.0005461Tainá Raiol - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado em mais de uma unidade da Fiocruz, mas não consta à informação no documento do vinculo com a Fiocruz Brasília.University of Brasília. School of Medicine. Molecular Pathology Post-Graduate Program. Brasília, DF, Brazil.University of Brasília. School of Medicine. Molecular Pathology Post-Graduate Program. Brasília, DF, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Brasília, DF, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Brasília, DF, Brazil.University of Brasília. University Hospital. Brasília, DF, Brazil.Fundação Oswaldo Cruz. Fiocruz Amazonas. Instituto Leônidas e Maria Deane Manaus, AM, Brasil.Federal University of Mato Grosso do Sul. School of Computing Sciences. Campo Grande, MS, Brazil.Federal University of Mato Grosso do Sul. School of Computing Sciences. Campo Grande, MS, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Brasília, DF, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Brasília, DF, Brazil / University of São Paulo. School of Pharmaceutical Sciences. Department of Food Sciences and Experimental Nutrition, São Paulo, SP, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Brasília, DF, Brazil.A common theme across multiple fungal pathogens is their ability to impair the establishment of a protective immune response. Although early inflammation is beneficial in containing the infection, an uncontrolled inflammatory response is detrimental and may eventually oppose disease eradication. Chromoblastomycosis (CBM), a cutaneous and subcutaneous mycosis, caused by dematiaceous fungi, is capable of inducing a chronic inflammatory response. Muriform cells, the parasitic form of Fonsecaea pedrosoi, are highly prevalent in infected tissues, especially in long-standing lesions. In this study we show that hyphae and muriform cells are able to establish a murine CBM with skin lesions and histopathological aspects similar to that found in humans, with muriform cells being the most persistent fungal form, whereas mice infected with conidia do not reach the chronic phase of the disease. Moreover, in injured tissue the presence of hyphae and especially muriform cells, but not conidia, is correlated with intense production of pro-inflammatory cytokines in vivo. High-throughput RNA sequencing analysis (RNA-Seq) performed at early time points showed a strong up-regulation of genes related to fungal recognition, cell migration, inflammation, apoptosis and phagocytosis in macrophages exposed in vitro to muriform cells, but not conidia. We also demonstrate that only muriform cells required FcγR and Dectin-1 recognition to be internalized in vitro, and this is the main fungal form responsible for the intense inflammatory pattern observed in CBM, clarifying the chronic inflammatory reaction observed in most patients. Furthermore, our findings reveal two different fungal-host interaction strategies according to fungal morphotype, highlighting fungal dimorphism as an important key in understanding the bipolar nature of inflammatory response in fungal infections.engPublic Library of ScienceApoptosisAscomycotaChromoblastomycosisCytokinesDisease Models, AnimalHigh-Throughput Nucleotide SequencingHyphaeLectins, C-TypeMacrophagesMiceMice, Inbred BALB CPhagocytosisSpores, FungalUp-RegulationHost-Pathogen InteractionsMacrophagesFungal diseasesGene expressionInflammationInflammatory diseasesCytokinesFungal geneticsPhagocytosisApoptoseAscomicetosCromoblastomicoseCitocinasModelos Animais de DoençasSequenciamento de Nucleotídeos em Larga EscalaHifasLectinas Tipo CMacrófagosCamundongos Endogâmicos BALB CFagocitoseAnálise de Sequência de RNAInterações Hospedeiro-PatógenoEsporos FúngicosModulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity |
| title |
Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity |
| spellingShingle |
Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity Siqueira, Isaque Medeiros Apoptosis Ascomycota Chromoblastomycosis Cytokines Disease Models, Animal High-Throughput Nucleotide Sequencing Hyphae Lectins, C-Type Macrophages Mice Mice, Inbred BALB C Phagocytosis Spores, Fungal Up-Regulation Host-Pathogen Interactions Macrophages Fungal diseases Gene expression Inflammation Inflammatory diseases Cytokines Fungal genetics Phagocytosis Apoptose Ascomicetos Cromoblastomicose Citocinas Modelos Animais de Doenças Sequenciamento de Nucleotídeos em Larga Escala Hifas Lectinas Tipo C Macrófagos Camundongos Endogâmicos BALB C Fagocitose Análise de Sequência de RNA Interações Hospedeiro-Patógeno Esporos Fúngicos |
| title_short |
Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity |
| title_full |
Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity |
| title_fullStr |
Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity |
| title_full_unstemmed |
Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity |
| title_sort |
Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity |
| author |
Siqueira, Isaque Medeiros |
| author_facet |
Siqueira, Isaque Medeiros Castro, Raffael Júnio Araújo de Leonhardt, Luiza Chaves de Miranda Jerônimo, Márcio Sousa Soares, Aluízio Carlos Raiol, Tainá Nishibe, Christiane Almeida, Nalvo Tavares, Aldo Henrique Hoffmann, Christian Bocca, Anamelia Lorenzetti |
| author_role |
author |
| author2 |
Castro, Raffael Júnio Araújo de Leonhardt, Luiza Chaves de Miranda Jerônimo, Márcio Sousa Soares, Aluízio Carlos Raiol, Tainá Nishibe, Christiane Almeida, Nalvo Tavares, Aldo Henrique Hoffmann, Christian Bocca, Anamelia Lorenzetti |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Siqueira, Isaque Medeiros Castro, Raffael Júnio Araújo de Leonhardt, Luiza Chaves de Miranda Jerônimo, Márcio Sousa Soares, Aluízio Carlos Raiol, Tainá Nishibe, Christiane Almeida, Nalvo Tavares, Aldo Henrique Hoffmann, Christian Bocca, Anamelia Lorenzetti |
| dc.subject.mesh.pt_BR.fl_str_mv |
Apoptosis Ascomycota Chromoblastomycosis Cytokines Disease Models, Animal High-Throughput Nucleotide Sequencing Hyphae Lectins, C-Type Macrophages Mice Mice, Inbred BALB C Phagocytosis Spores, Fungal Up-Regulation Host-Pathogen Interactions |
| topic |
Apoptosis Ascomycota Chromoblastomycosis Cytokines Disease Models, Animal High-Throughput Nucleotide Sequencing Hyphae Lectins, C-Type Macrophages Mice Mice, Inbred BALB C Phagocytosis Spores, Fungal Up-Regulation Host-Pathogen Interactions Macrophages Fungal diseases Gene expression Inflammation Inflammatory diseases Cytokines Fungal genetics Phagocytosis Apoptose Ascomicetos Cromoblastomicose Citocinas Modelos Animais de Doenças Sequenciamento de Nucleotídeos em Larga Escala Hifas Lectinas Tipo C Macrófagos Camundongos Endogâmicos BALB C Fagocitose Análise de Sequência de RNA Interações Hospedeiro-Patógeno Esporos Fúngicos |
| dc.subject.en.pt_BR.fl_str_mv |
Macrophages Fungal diseases Gene expression Inflammation Inflammatory diseases Cytokines Fungal genetics Phagocytosis |
| dc.subject.decs.pt_BR.fl_str_mv |
Apoptose Ascomicetos Cromoblastomicose Citocinas Modelos Animais de Doenças Sequenciamento de Nucleotídeos em Larga Escala Hifas Lectinas Tipo C Macrófagos Camundongos Endogâmicos BALB C Fagocitose Análise de Sequência de RNA Interações Hospedeiro-Patógeno Esporos Fúngicos |
| description |
Tainá Raiol - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado em mais de uma unidade da Fiocruz, mas não consta à informação no documento do vinculo com a Fiocruz Brasília. |
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2017 |
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2017 |
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2020-08-12T13:33:52Z |
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2020-08-12T13:33:52Z |
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SIQUEIRA, Isaque Medeiros et al. Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity. PloS neglected tropical diseases, [San Francisco], v.11, n.3, p. 1-23, 2017. |
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https://www.arca.fiocruz.br/handle/icict/42710 |
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1935-2727 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0005461 |
| identifier_str_mv |
SIQUEIRA, Isaque Medeiros et al. Modulation of the immune response by Fonsecaea pedrosoi morphotypes in the course of experimental chromoblastomycosis and their role on inflammatory response chronicity. PloS neglected tropical diseases, [San Francisco], v.11, n.3, p. 1-23, 2017. 1935-2727 10.1371/journal.pntd.0005461 |
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Public Library of Science |
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