Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host

Detalhes bibliográficos
Autor(a) principal: Peterson, Greice Krautz
Data de Publicação: 2010
Outros Autores: Simoes, Mariana, Faghiri, Zahra, Ndegwa, David, Oliveira, Guilherme Corrêa de, Shoemaker, Charles B., Skelly, Patrick J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5642
Resumo: Tufts University. Cummings School of Veterinary Medicine. Department of Biomedical Sciences. Molecular Helminthology Laboratory. Division of Infectious Diseases. Grafton, Massachusetts, United States of America
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spelling Peterson, Greice KrautzSimoes, MarianaFaghiri, ZahraNdegwa, DavidOliveira, Guilherme Corrêa deShoemaker, Charles B.Skelly, Patrick J.2012-10-04T18:12:19Z2012-10-04T18:12:19Z2010KRAUTZ-PETERSON, Greice et al. (2010) Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host. PLoS Pathog 6(6): e100093215537366https://www.arca.fiocruz.br/handle/icict/564210.1371/journal.ppat.1000932engPublic Library of Science (PLoS) 15537366Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Hostinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTufts University. Cummings School of Veterinary Medicine. Department of Biomedical Sciences. Molecular Helminthology Laboratory. Division of Infectious Diseases. Grafton, Massachusetts, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou Laboratório de Imunologia Celular e Molecular e CEBio. Belo Horizonte, MG, BrazilTufts University. Cummings School of Veterinary Medicine. Department of Biomedical Sciences. Molecular Helminthology Laboratory. Division of Infectious Diseases. Grafton, Massachusetts, United States of AmericaTufts University. Cummings School of Veterinary Medicine. Department of Biomedical Sciences. Molecular Helminthology Laboratory. Division of Infectious Diseases. Grafton, Massachusetts, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou Laboratório de Imunologia Celular e Molecular e CEBio. Belo Horizonte, MG, Brazil/ Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, BrazilTufts University. Cummings School of Veterinary Medicine. Department of Biomedical Sciences. Molecular Helminthology Laboratory. Division of Infectious Diseases. Grafton, Massachusetts, United States of AmericaTufts University. Cummings School of Veterinary Medicine. Department of Biomedical Sciences. Molecular Helminthology Laboratory. Division of Infectious Diseases. Grafton, Massachusetts, United States of America.Adult schistosomes live in the host’s bloodstream where they import nutrients such as glucose across their body surface (the tegument). The parasite tegument is an unusual structure since it is enclosed not by the typical one but by two closely apposed lipid bilayers. Within the tegument two glucose importing proteins have been identified; these are schistosome glucose transporter (SGTP) 1 and 4. SGTP4 is present in the host interactive, apical tegumental membranes, while SGTP1 is found in the tegumental basal membrane (as well as in internal tissues). The SGTPs act by facilitated diffusion. To examine the importance of these proteins for the parasites, RNAi was employed to knock down expression of both SGTP genes in the schistosomula and adult worm life stages. Both qRT-PCR and western blotting analysis confirmed successful gene suppression. It was found that SGTP1 or SGTP4-suppressed parasites exhibit an impaired ability to import glucose compared to control worms. In addition, parasites with both SGTP1 and SGTP4 simultaneously suppressed showed a further reduction in capacity to import glucose compared to parasites with a single suppressed SGTP gene. Despite this debility, all suppressed parasites exhibited no phenotypic distinction compared to controls when cultured in rich medium. Following prolonged incubation in glucose-depleted medium however, significantly fewer SGTP-suppressed parasites survived. Finally, SGTP-suppressed parasites showed decreased viability in vivo following infection of experimental animals. These findings provide direct evidence for the importance of SGTP1 and SGTP4 for schistosomes in importing exogenous glucose and show that these proteins are important for normal parasite development in the mammalian host.Schistosoma mansoniSchistosoma mansoni glucose transporterinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL64.2010.pdf64.2010.pdfapplication/pdf700080https://www.arca.fiocruz.br/bitstream/icict/5642/1/64.2010.pdf9c87ca5e7ce8bbdfa927c50c54eeb899MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5642/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXT64.2010.pdf.txt64.2010.pdf.txtExtracted texttext/plain41737https://www.arca.fiocruz.br/bitstream/icict/5642/5/64.2010.pdf.txt170c7caebf9cea839139acdc1d48bba8MD55THUMBNAIL64.2010.pdf.jpg64.2010.pdf.jpgGenerated Thumbnailimage/jpeg2540https://www.arca.fiocruz.br/bitstream/icict/5642/4/64.2010.pdf.jpgedfa6d2a6675ee6d78bc3f49153cb486MD54icict/56422018-05-18 11:39:45.273oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-05-18T14:39:45Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host
title Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host
spellingShingle Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host
Peterson, Greice Krautz
Schistosoma mansoni
Schistosoma mansoni glucose transporter
title_short Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host
title_full Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host
title_fullStr Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host
title_full_unstemmed Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host
title_sort Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host
author Peterson, Greice Krautz
author_facet Peterson, Greice Krautz
Simoes, Mariana
Faghiri, Zahra
Ndegwa, David
Oliveira, Guilherme Corrêa de
Shoemaker, Charles B.
Skelly, Patrick J.
author_role author
author2 Simoes, Mariana
Faghiri, Zahra
Ndegwa, David
Oliveira, Guilherme Corrêa de
Shoemaker, Charles B.
Skelly, Patrick J.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Peterson, Greice Krautz
Simoes, Mariana
Faghiri, Zahra
Ndegwa, David
Oliveira, Guilherme Corrêa de
Shoemaker, Charles B.
Skelly, Patrick J.
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Schistosoma mansoni glucose transporter
topic Schistosoma mansoni
Schistosoma mansoni glucose transporter
description Tufts University. Cummings School of Veterinary Medicine. Department of Biomedical Sciences. Molecular Helminthology Laboratory. Division of Infectious Diseases. Grafton, Massachusetts, United States of America
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2012-10-04T18:12:19Z
dc.date.available.fl_str_mv 2012-10-04T18:12:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv KRAUTZ-PETERSON, Greice et al. (2010) Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host. PLoS Pathog 6(6): e1000932
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5642
dc.identifier.issn.none.fl_str_mv 15537366
dc.identifier.doi.none.fl_str_mv 10.1371/journal.ppat.1000932
identifier_str_mv KRAUTZ-PETERSON, Greice et al. (2010) Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host. PLoS Pathog 6(6): e1000932
15537366
10.1371/journal.ppat.1000932
url https://www.arca.fiocruz.br/handle/icict/5642
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Public Library of Science (PLoS) 15537366
publisher.none.fl_str_mv Public Library of Science (PLoS) 15537366
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instname:Fundação Oswaldo Cruz (FIOCRUZ)
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