Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42397 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Sinalização Celular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil. |
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Santos, Carlucio Rocha dosRodovalho, Cynara de MeloJablonka, WillyMartins, Ademir JesusLima, José Bento PereiraDias, Luciana dos SantosSilva Neto, Mário Alberto Cardoso da SilvaAtella, Georgia Correa2020-07-25T19:02:07Z2020-07-25T19:02:07Z2020SANTOS, Carlucio Rocha dos et al. Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil. Parasites Vectors, v. 13, n. 293, p. 1-14, 2020.1756-3305https://www.arca.fiocruz.br/handle/icict/4239710.1186/s13071-020-04166-3engBMCAedes aegyptiMosquitoResistência a InseticidasEsforçoAedes aegyptiMosquitoInsecticide resistanceFitnessInsecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Sinalização Celular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Sinalização Celular. Rio de Janeiro, RJ, Brasil,Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Sinalização Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Lipídios e Lipoproteína. Rio de Janeiro, RJ, Brasil.Background: Aedes aegypti is a vector of high relevance, since it transmits several arboviruses, including dengue, chikungunya and Zika. Studies on vector biology are usually conducted with laboratory strains presenting a divergent genetic composition from feld populations. This may impair vector control policies that were based on laboratory observations employing only long maintained laboratory strains. In the present study we characterized a laboratory strain interbreed with Ae. aegypti collected from fve diferent localities in Rio de Janeiro (Aedes Rio), for insecticide resistance (IR), IR mechanisms, ftness and Zika virus infection. Methods: We compared the recently established Aedes Rio with the laboratory reference strain Rockefeller. Insecticide resistance (deltamethrin, malathion and temephos), activity of metabolic resistance enzymes and kdr mutation frequency were determined. Some life table parameters (longevity, blood-feeding, number and egg viability) and Zika virus susceptibility was also determined. Results: Aedes Rio showed resistance to deltamethrin (resistance ratio, RR50=32.6) and temephos (RR50=7.0) and elevated activity of glutathione S-transferase (GST) and esterases (α-EST and pNPA-EST), but not acetylcholinesterase (AChE). In total, 92.1% of males genotyped for kdr presented a “resistant” genotype. Weekly blood-fed females from both strains, presented reduced mortality compared to sucrose-fed mosquitoes; however, Aedes Rio blood-fed females did not live as long (mean lifespan: Rockefeller=70±3.07; Aedes Rio=53.5±2.16 days). There were no diferences between strains in relation to blood-feeding and number of eggs, but Aedes Rio eggs presented reduced viability (mean hatch: Rockefeller=77.79±1.4%; Aedes Rio=58.57±1.77%). Zika virus infection (plaque-forming unit, PFU) was similar in both strains (mean PFU±SE: Aedes Rio: 4.53×104±1.14×104 PFU; Rockefeller: 2.02×104±0.71×104 PFU). Conclusion: Selected conditions in the feld, such as IR mechanisms, may result in pleiotropic efects that interfere in general physiology of the insect. Therefore, it is important to well characterize feld populations to be tested in parallel with laboratory reference strains. This practice would improve the signifcance of laboratory tests for vector control methods.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42397/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarlucioRSantos_CynaraRodovalho_etal_IOC_2020.pdfCarlucioRSantos_CynaraRodovalho_etal_IOC_2020.pdfapplication/pdf1444075https://www.arca.fiocruz.br/bitstream/icict/42397/2/CarlucioRSantos_CynaraRodovalho_etal_IOC_2020.pdf6ba4613cfeecc910b11b569f1a6f16d4MD52TEXTCarlucioRSantos_CynaraRodovalho_etal_IOC_2020.pdf.txtCarlucioRSantos_CynaraRodovalho_etal_IOC_2020.pdf.txtExtracted texttext/plain70079https://www.arca.fiocruz.br/bitstream/icict/42397/3/CarlucioRSantos_CynaraRodovalho_etal_IOC_2020.pdf.txte2c6c7323b7d1388d1acce9c8f5ecf21MD53icict/423972023-09-05 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| dc.title.pt_BR.fl_str_mv |
Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil |
| title |
Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil |
| spellingShingle |
Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil Santos, Carlucio Rocha dos Aedes aegypti Mosquito Resistência a Inseticidas Esforço Aedes aegypti Mosquito Insecticide resistance Fitness |
| title_short |
Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil |
| title_full |
Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil |
| title_fullStr |
Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil |
| title_full_unstemmed |
Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil |
| title_sort |
Insecticide resistance, ftness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil |
| author |
Santos, Carlucio Rocha dos |
| author_facet |
Santos, Carlucio Rocha dos Rodovalho, Cynara de Melo Jablonka, Willy Martins, Ademir Jesus Lima, José Bento Pereira Dias, Luciana dos Santos Silva Neto, Mário Alberto Cardoso da Silva Atella, Georgia Correa |
| author_role |
author |
| author2 |
Rodovalho, Cynara de Melo Jablonka, Willy Martins, Ademir Jesus Lima, José Bento Pereira Dias, Luciana dos Santos Silva Neto, Mário Alberto Cardoso da Silva Atella, Georgia Correa |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Carlucio Rocha dos Rodovalho, Cynara de Melo Jablonka, Willy Martins, Ademir Jesus Lima, José Bento Pereira Dias, Luciana dos Santos Silva Neto, Mário Alberto Cardoso da Silva Atella, Georgia Correa |
| dc.subject.other.pt_BR.fl_str_mv |
Aedes aegypti Mosquito Resistência a Inseticidas Esforço |
| topic |
Aedes aegypti Mosquito Resistência a Inseticidas Esforço Aedes aegypti Mosquito Insecticide resistance Fitness |
| dc.subject.en.pt_BR.fl_str_mv |
Aedes aegypti Mosquito Insecticide resistance Fitness |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Sinalização Celular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020-07-25T19:02:07Z |
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2020-07-25T19:02:07Z |
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2020 |
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SANTOS, Carlucio Rocha dos et al. Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil. Parasites Vectors, v. 13, n. 293, p. 1-14, 2020. |
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https://www.arca.fiocruz.br/handle/icict/42397 |
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1756-3305 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s13071-020-04166-3 |
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SANTOS, Carlucio Rocha dos et al. Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil. Parasites Vectors, v. 13, n. 293, p. 1-14, 2020. 1756-3305 10.1186/s13071-020-04166-3 |
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