Multispacer Sequence Typing for Mycobacterium bovis Genotyping

Detalhes bibliográficos
Autor(a) principal: Sales, Érica Bravo
Data de Publicação: 2021
Outros Autores: Fonseca Jr., Antônio Augusto, Gonçalves, Cristina Magalhães, Lage, Andrey Pereira, Andrade, Giovanna Ivo, Suffys, Philip Noel, Gomes, Harrison Magdinier, Dias, Natanael Lamas, Ferreira Neto, José Soares, Guimarães, Ana Marcia de Sá, Heinemann, Marcos Bryan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50128
Resumo: Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil.
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spelling Sales, Érica BravoFonseca Jr., Antônio AugustoGonçalves, Cristina MagalhãesLage, Andrey PereiraAndrade, Giovanna IvoSuffys, Philip NoelGomes, Harrison MagdinierDias, Natanael LamasFerreira Neto, José SoaresGuimarães, Ana Marcia de SáHeinemann, Marcos Bryan2021-12-01T19:58:45Z2021-12-01T19:58:45Z2021SALES, Érica Bravo et al. Multispacer Sequence Typing for Mycobacterium bovis Genotyping. Frontiers in Veterinary Science, v. 8, Article 666283, p. 1 - 12, April 2021.2299-1769https://www.arca.fiocruz.br/handle/icict/5012810.3389/fvets.2021.666283engFrontiers MediaMycobactereium bovisTuberculose bovinaGenótipoEpidemologiaMSTMycobactereium bovisBovine tuberculosisGenotypeEpidemiologyMSTMultispacer Sequence Typing for Mycobacterium bovis Genotypinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil.Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil.Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.The molecular typing of Mycobacterium bovis, which causes bovine tuberculosis, can be accomplished by combining different polymorphic markers, contributing to its epidemiological investigation. Multispacer sequence typing (MST) is a sequencing-based method that employs intergenic regions susceptible to higher mutation rates given the low selection pressure. It has been applied to M. tuberculosis, but not to M. bovis. The aim of this study was to evaluate a MST for M. bovis. A total of 58 strains isolated from tissues with lesions suggestive of bovine tuberculosis, coming from cattle herds in six Brazilian states and four standard samples of M. bovis were typified employing the MST technique. Fourteen intergenic regions were used, and four types of genetic events were reported: single nucleotide mutation (SNP), insertion, deletion, and tandem repeat (TR). Seven loci were chosen for typing. Twenty-eight type sequences (ST) were identified, indicating type sequences (ST) were identified, indicating a 92.9% HGDI (Hunter Gaston Discriminatory Index). The data were used to analyze the evolutionary patterns of these isolates and correlate them to phylogeographic lineages based on the formation of clonal complexes generated from eBURST software. Later, we associated the MST with spoligotyping technique, currently considered the gold standard for classification of M. bovis. The results support the MST as an alternative method for genotyping of M. bovis. The method has the advantage of sequencing and the availability of sequences analyzed in public databases, which can be used by professionals around the world as a tool for further analysis. This was the first study to identify the variability of isolates of M. bovis by the MST method.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50128/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPhilipSuffys_HarisonGomes_etal_IOC_2021.pdfPhilipSuffys_HarisonGomes_etal_IOC_2021.pdfapplication/pdf2338344https://www.arca.fiocruz.br/bitstream/icict/50128/2/PhilipSuffys_HarisonGomes_etal_IOC_2021.pdf1bb920ba6701de07b3a9f0449d97f674MD52icict/501282021-12-01 16:58:45.204oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-12-01T19:58:45Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Multispacer Sequence Typing for Mycobacterium bovis Genotyping
title Multispacer Sequence Typing for Mycobacterium bovis Genotyping
spellingShingle Multispacer Sequence Typing for Mycobacterium bovis Genotyping
Sales, Érica Bravo
Mycobactereium bovis
Tuberculose bovina
Genótipo
Epidemologia
MST
Mycobactereium bovis
Bovine tuberculosis
Genotype
Epidemiology
MST
title_short Multispacer Sequence Typing for Mycobacterium bovis Genotyping
title_full Multispacer Sequence Typing for Mycobacterium bovis Genotyping
title_fullStr Multispacer Sequence Typing for Mycobacterium bovis Genotyping
title_full_unstemmed Multispacer Sequence Typing for Mycobacterium bovis Genotyping
title_sort Multispacer Sequence Typing for Mycobacterium bovis Genotyping
author Sales, Érica Bravo
author_facet Sales, Érica Bravo
Fonseca Jr., Antônio Augusto
Gonçalves, Cristina Magalhães
Lage, Andrey Pereira
Andrade, Giovanna Ivo
Suffys, Philip Noel
Gomes, Harrison Magdinier
Dias, Natanael Lamas
Ferreira Neto, José Soares
Guimarães, Ana Marcia de Sá
Heinemann, Marcos Bryan
author_role author
author2 Fonseca Jr., Antônio Augusto
Gonçalves, Cristina Magalhães
Lage, Andrey Pereira
Andrade, Giovanna Ivo
Suffys, Philip Noel
Gomes, Harrison Magdinier
Dias, Natanael Lamas
Ferreira Neto, José Soares
Guimarães, Ana Marcia de Sá
Heinemann, Marcos Bryan
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sales, Érica Bravo
Fonseca Jr., Antônio Augusto
Gonçalves, Cristina Magalhães
Lage, Andrey Pereira
Andrade, Giovanna Ivo
Suffys, Philip Noel
Gomes, Harrison Magdinier
Dias, Natanael Lamas
Ferreira Neto, José Soares
Guimarães, Ana Marcia de Sá
Heinemann, Marcos Bryan
dc.subject.other.pt_BR.fl_str_mv Mycobactereium bovis
Tuberculose bovina
Genótipo
Epidemologia
MST
topic Mycobactereium bovis
Tuberculose bovina
Genótipo
Epidemologia
MST
Mycobactereium bovis
Bovine tuberculosis
Genotype
Epidemiology
MST
dc.subject.en.pt_BR.fl_str_mv Mycobactereium bovis
Bovine tuberculosis
Genotype
Epidemiology
MST
description Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-12-01T19:58:45Z
dc.date.available.fl_str_mv 2021-12-01T19:58:45Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SALES, Érica Bravo et al. Multispacer Sequence Typing for Mycobacterium bovis Genotyping. Frontiers in Veterinary Science, v. 8, Article 666283, p. 1 - 12, April 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50128
dc.identifier.issn.pt_BR.fl_str_mv 2299-1769
dc.identifier.doi.none.fl_str_mv 10.3389/fvets.2021.666283
identifier_str_mv SALES, Érica Bravo et al. Multispacer Sequence Typing for Mycobacterium bovis Genotyping. Frontiers in Veterinary Science, v. 8, Article 666283, p. 1 - 12, April 2021.
2299-1769
10.3389/fvets.2021.666283
url https://www.arca.fiocruz.br/handle/icict/50128
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50128/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50128/2/PhilipSuffys_HarisonGomes_etal_IOC_2021.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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