Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix

Detalhes bibliográficos
Autor(a) principal: Logullo, Jorgete
Data de Publicação: 2023
Outros Autores: LIma, Israel Diniz, Rocha, Juliana Dutra B., Cortê-Real, Suzana, Silva-Júnior, Elias Barbosa da, Oliveira, Joyce Cristina Guimarães de, Morrot, Alexandre, Fonseca, Leonardo Marques da, Lima, Leonardo Freire de, Decote-Ricardo, Debora, Lima, Celio Geraldo Freire de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58083
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
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spelling Logullo, JorgeteLIma, Israel DinizRocha, Juliana Dutra B.Cortê-Real, SuzanaSilva-Júnior, Elias Barbosa daOliveira, Joyce Cristina Guimarães deMorrot, AlexandreFonseca, Leonardo Marques daLima, Leonardo Freire deDecote-Ricardo, DeboraLima, Celio Geraldo Freire de2023-04-29T14:28:58Z2023-04-29T14:28:58Z2023LOGULLO, Jorgete et al. Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix. Life, v.13, 1063, p. 11 - 18, Apr. 2023.2075-1729https://www.arca.fiocruz.br/handle/icict/5808310.3390/life13041063engMDPIMatriz extracelularMacrófagosTrypanosoma cruziColágeno IMatriz 3DExtracellular matrixMacrophagesTrypanosoma cruziCollagen I3D matrixIncreased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrixinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitogia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.The interactions between cell and cellular matrix confers plasticity to each body tissue, influencing the cellular migratory capacity. Macrophages rely on motility to promote their physiological function. These phagocytes are determinant for the control of invasive infections, and their immunological role largely depends on their ability to migrate and adhere to tissue. Therefore, they interact with the components of the extracellular matrix through their adhesion receptors, conferring morphological modifications that change their shape during migration. Nevertheless, the need to use in vitro cell growth models with the conditioning of three-dimensional synthetic matrices to mimic the dynamics of cell-matrix interaction has been increasingly studied. This becomes more important to effectively understand the changes occurring in phagocyte morphology in the context of infection progression, such as in Chagas disease. This disease is caused by the intracellular pathogen Trypanosoma cruzi, capable of infecting macrophages, determinant cells in the anti-trypanosomatid immunity. In the present study, we sought to understand how an in vitro extracellular matrix model interferes with T. cruzi infection in macrophages. Using different time intervals and parasite ratios, we evaluated the cell morphology and parasite replication rate in the presence of 3D collagen I matrix. Nevertheless, microscopy techniques such as scanning electron microscopy were crucial to trace macrophage-matrix interactions. In the present work, we demonstrated for the first time that the macrophage-matrix interaction favors T. cruzi in vitro replication and the release of anti-inflammatory cytokines during macrophage infection, in addition to drastically altering the morphology of the macrophages and promoting the formation of migratory macrophages.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58083/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSuzanaCorteReal_AlexandreMorrot_etal_IOC_2023.pdfSuzanaCorteReal_AlexandreMorrot_etal_IOC_2023.pdfapplication/pdf3362646https://www.arca.fiocruz.br/bitstream/icict/58083/2/SuzanaCorteReal_AlexandreMorrot_etal_IOC_2023.pdf303089311648d6052c762de5a593d6d2MD52icict/580832023-09-14 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dc.title.en_US.fl_str_mv Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix
title Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix
spellingShingle Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix
Logullo, Jorgete
Matriz extracelular
Macrófagos
Trypanosoma cruzi
Colágeno I
Matriz 3D
Extracellular matrix
Macrophages
Trypanosoma cruzi
Collagen I
3D matrix
title_short Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix
title_full Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix
title_fullStr Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix
title_full_unstemmed Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix
title_sort Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix
author Logullo, Jorgete
author_facet Logullo, Jorgete
LIma, Israel Diniz
Rocha, Juliana Dutra B.
Cortê-Real, Suzana
Silva-Júnior, Elias Barbosa da
Oliveira, Joyce Cristina Guimarães de
Morrot, Alexandre
Fonseca, Leonardo Marques da
Lima, Leonardo Freire de
Decote-Ricardo, Debora
Lima, Celio Geraldo Freire de
author_role author
author2 LIma, Israel Diniz
Rocha, Juliana Dutra B.
Cortê-Real, Suzana
Silva-Júnior, Elias Barbosa da
Oliveira, Joyce Cristina Guimarães de
Morrot, Alexandre
Fonseca, Leonardo Marques da
Lima, Leonardo Freire de
Decote-Ricardo, Debora
Lima, Celio Geraldo Freire de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Logullo, Jorgete
LIma, Israel Diniz
Rocha, Juliana Dutra B.
Cortê-Real, Suzana
Silva-Júnior, Elias Barbosa da
Oliveira, Joyce Cristina Guimarães de
Morrot, Alexandre
Fonseca, Leonardo Marques da
Lima, Leonardo Freire de
Decote-Ricardo, Debora
Lima, Celio Geraldo Freire de
dc.subject.other.en_US.fl_str_mv Matriz extracelular
Macrófagos
Trypanosoma cruzi
Colágeno I
Matriz 3D
topic Matriz extracelular
Macrófagos
Trypanosoma cruzi
Colágeno I
Matriz 3D
Extracellular matrix
Macrophages
Trypanosoma cruzi
Collagen I
3D matrix
dc.subject.en.en_US.fl_str_mv Extracellular matrix
Macrophages
Trypanosoma cruzi
Collagen I
3D matrix
description Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-04-29T14:28:58Z
dc.date.available.fl_str_mv 2023-04-29T14:28:58Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LOGULLO, Jorgete et al. Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix. Life, v.13, 1063, p. 11 - 18, Apr. 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58083
dc.identifier.issn.en_US.fl_str_mv 2075-1729
dc.identifier.doi.none.fl_str_mv 10.3390/life13041063
identifier_str_mv LOGULLO, Jorgete et al. Increased Trypanosoma cruzi Growth during Infection of Macrophages Cultured on Collagen I Matrix. Life, v.13, 1063, p. 11 - 18, Apr. 2023.
2075-1729
10.3390/life13041063
url https://www.arca.fiocruz.br/handle/icict/58083
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/58083/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/58083/2/SuzanaCorteReal_AlexandreMorrot_etal_IOC_2023.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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