Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/47886 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. |
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Carramaschi, Isabel NogueiraLopes, Jonathan Christian O.Leite, Jéssica AlbuquerqueCarneiro, Marcos TavaresBarbosa, Rodrigo RochaVillas Boas, Maria HelenaRangel, KaryneChagas, Thiago Pavoni GomesQueiroz, Margareth M. C.Zaher, Viviane2021-06-27T12:22:45Z2021-06-27T12:22:45Z2021CARRAMASCHI, Isabel Nogueira et al. Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city. Acta Tropica, v. 220, 105962, 8 p, May 2021.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/4788610.1016/j.actatropica.2021.105962engElsevierDipteranos muscoidesGenes de resistênciaSaúde ÚnicaGenes MDRMuscoid dipteransResistance genesOne healthGenes MDRGene de Resistência a MultidrogasSurveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro cityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Laboratório de Microbiologia do Departamento de Saneamento e Saúde Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Centro Universitário de Volta Redonda. Volta Redonda, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Laboratório de Saneantes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Antimicrobial-resistant bacteria were isolated from muscoid dipterans collected at five different areas of Rio de Janeiro city, in proximity to hospitals. Extracts obtained by maceration of flies were diluted and used as inocula for different culture media, with or without antibiotic (ceftriaxone 1 mg/L) supplementation. Purified isolates were submitted to antimicrobial susceptibility testing (AST). Bacterial identification was performed by MALDI TOF Microflex LT (Bruker Daltonics). A total of 197 bacterial strains were obtained from 117 dipterous muscoids. Forty-two flies (35.9%) carried bacteria resistant to at least one antimicrobial, while 7 insects (5.9%) carried multidrug-resistant bacteria (MDR), which were all members of the family Enterobacteriaceae. Among 10 MDR bacteria (5%), 5 strains (2,5%) were positive by PCR for one or more of the following antibiotic resistance genes: aac(6’)-Ib, blaTEM-1, blaCTX-M-15, blaKPC-2 and blaNDM-1. Analysis of variance (ANOVA) and cluster analysis compared the number of resistant isolates per collection point and showed that a single location was statistically different from the others with regard to resistance. Although there are still no criteria to determine the envi ronmental contamination by resistant bacteria the fact that they have been isolated from flies is an indication of a disseminated contamination. As such, these insects may be useful in monitoring programs of antibiotic resistance in non-hospital environments, where they could function as sentinels.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47886/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdfIsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdfapplication/pdf659037https://www.arca.fiocruz.br/bitstream/icict/47886/2/IsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdfbc3c531419da9f31e91780f7f09f90a8MD52TEXTIsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdf.txtIsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city |
title |
Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city |
spellingShingle |
Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city Carramaschi, Isabel Nogueira Dipteranos muscoides Genes de resistência Saúde Única Genes MDR Muscoid dipterans Resistance genes One health Genes MDR Gene de Resistência a Multidrogas |
title_short |
Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city |
title_full |
Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city |
title_fullStr |
Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city |
title_full_unstemmed |
Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city |
title_sort |
Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city |
author |
Carramaschi, Isabel Nogueira |
author_facet |
Carramaschi, Isabel Nogueira Lopes, Jonathan Christian O. Leite, Jéssica Albuquerque Carneiro, Marcos Tavares Barbosa, Rodrigo Rocha Villas Boas, Maria Helena Rangel, Karyne Chagas, Thiago Pavoni Gomes Queiroz, Margareth M. C. Zaher, Viviane |
author_role |
author |
author2 |
Lopes, Jonathan Christian O. Leite, Jéssica Albuquerque Carneiro, Marcos Tavares Barbosa, Rodrigo Rocha Villas Boas, Maria Helena Rangel, Karyne Chagas, Thiago Pavoni Gomes Queiroz, Margareth M. C. Zaher, Viviane |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Carramaschi, Isabel Nogueira Lopes, Jonathan Christian O. Leite, Jéssica Albuquerque Carneiro, Marcos Tavares Barbosa, Rodrigo Rocha Villas Boas, Maria Helena Rangel, Karyne Chagas, Thiago Pavoni Gomes Queiroz, Margareth M. C. Zaher, Viviane |
dc.subject.other.pt_BR.fl_str_mv |
Dipteranos muscoides Genes de resistência Saúde Única Genes MDR |
topic |
Dipteranos muscoides Genes de resistência Saúde Única Genes MDR Muscoid dipterans Resistance genes One health Genes MDR Gene de Resistência a Multidrogas |
dc.subject.en.pt_BR.fl_str_mv |
Muscoid dipterans Resistance genes One health |
dc.subject.decs.pt_BR.fl_str_mv |
Genes MDR Gene de Resistência a Multidrogas |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-06-27T12:22:45Z |
dc.date.available.fl_str_mv |
2021-06-27T12:22:45Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARRAMASCHI, Isabel Nogueira et al. Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city. Acta Tropica, v. 220, 105962, 8 p, May 2021. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/47886 |
dc.identifier.issn.pt_BR.fl_str_mv |
0001-706X |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.actatropica.2021.105962 |
identifier_str_mv |
CARRAMASCHI, Isabel Nogueira et al. Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city. Acta Tropica, v. 220, 105962, 8 p, May 2021. 0001-706X 10.1016/j.actatropica.2021.105962 |
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https://www.arca.fiocruz.br/handle/icict/47886 |
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Elsevier |
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Elsevier |
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