Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city

Detalhes bibliográficos
Autor(a) principal: Carramaschi, Isabel Nogueira
Data de Publicação: 2021
Outros Autores: Lopes, Jonathan Christian O., Leite, Jéssica Albuquerque, Carneiro, Marcos Tavares, Barbosa, Rodrigo Rocha, Villas Boas, Maria Helena, Rangel, Karyne, Chagas, Thiago Pavoni Gomes, Queiroz, Margareth M. C., Zaher, Viviane
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/47886
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
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spelling Carramaschi, Isabel NogueiraLopes, Jonathan Christian O.Leite, Jéssica AlbuquerqueCarneiro, Marcos TavaresBarbosa, Rodrigo RochaVillas Boas, Maria HelenaRangel, KaryneChagas, Thiago Pavoni GomesQueiroz, Margareth M. C.Zaher, Viviane2021-06-27T12:22:45Z2021-06-27T12:22:45Z2021CARRAMASCHI, Isabel Nogueira et al. Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city. Acta Tropica, v. 220, 105962, 8 p, May 2021.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/4788610.1016/j.actatropica.2021.105962engElsevierDipteranos muscoidesGenes de resistênciaSaúde ÚnicaGenes MDRMuscoid dipteransResistance genesOne healthGenes MDRGene de Resistência a MultidrogasSurveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro cityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Laboratório de Microbiologia do Departamento de Saneamento e Saúde Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Centro Universitário de Volta Redonda. Volta Redonda, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Laboratório de Saneantes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Antimicrobial-resistant bacteria were isolated from muscoid dipterans collected at five different areas of Rio de Janeiro city, in proximity to hospitals. Extracts obtained by maceration of flies were diluted and used as inocula for different culture media, with or without antibiotic (ceftriaxone 1 mg/L) supplementation. Purified isolates were submitted to antimicrobial susceptibility testing (AST). Bacterial identification was performed by MALDI TOF Microflex LT (Bruker Daltonics). A total of 197 bacterial strains were obtained from 117 dipterous muscoids. Forty-two flies (35.9%) carried bacteria resistant to at least one antimicrobial, while 7 insects (5.9%) carried multidrug-resistant bacteria (MDR), which were all members of the family Enterobacteriaceae. Among 10 MDR bacteria (5%), 5 strains (2,5%) were positive by PCR for one or more of the following antibiotic resistance genes: aac(6’)-Ib, blaTEM-1, blaCTX-M-15, blaKPC-2 and blaNDM-1. Analysis of variance (ANOVA) and cluster analysis compared the number of resistant isolates per collection point and showed that a single location was statistically different from the others with regard to resistance. Although there are still no criteria to determine the envi ronmental contamination by resistant bacteria the fact that they have been isolated from flies is an indication of a disseminated contamination. As such, these insects may be useful in monitoring programs of antibiotic resistance in non-hospital environments, where they could function as sentinels.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47886/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdfIsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdfapplication/pdf659037https://www.arca.fiocruz.br/bitstream/icict/47886/2/IsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdfbc3c531419da9f31e91780f7f09f90a8MD52TEXTIsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdf.txtIsabelCarramaschi_VivianeZaher_etal_IOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
title Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
spellingShingle Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
Carramaschi, Isabel Nogueira
Dipteranos muscoides
Genes de resistência
Saúde Única
Genes MDR
Muscoid dipterans
Resistance genes
One health
Genes MDR
Gene de Resistência a Multidrogas
title_short Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
title_full Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
title_fullStr Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
title_full_unstemmed Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
title_sort Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city
author Carramaschi, Isabel Nogueira
author_facet Carramaschi, Isabel Nogueira
Lopes, Jonathan Christian O.
Leite, Jéssica Albuquerque
Carneiro, Marcos Tavares
Barbosa, Rodrigo Rocha
Villas Boas, Maria Helena
Rangel, Karyne
Chagas, Thiago Pavoni Gomes
Queiroz, Margareth M. C.
Zaher, Viviane
author_role author
author2 Lopes, Jonathan Christian O.
Leite, Jéssica Albuquerque
Carneiro, Marcos Tavares
Barbosa, Rodrigo Rocha
Villas Boas, Maria Helena
Rangel, Karyne
Chagas, Thiago Pavoni Gomes
Queiroz, Margareth M. C.
Zaher, Viviane
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carramaschi, Isabel Nogueira
Lopes, Jonathan Christian O.
Leite, Jéssica Albuquerque
Carneiro, Marcos Tavares
Barbosa, Rodrigo Rocha
Villas Boas, Maria Helena
Rangel, Karyne
Chagas, Thiago Pavoni Gomes
Queiroz, Margareth M. C.
Zaher, Viviane
dc.subject.other.pt_BR.fl_str_mv Dipteranos muscoides
Genes de resistência
Saúde Única
Genes MDR
topic Dipteranos muscoides
Genes de resistência
Saúde Única
Genes MDR
Muscoid dipterans
Resistance genes
One health
Genes MDR
Gene de Resistência a Multidrogas
dc.subject.en.pt_BR.fl_str_mv Muscoid dipterans
Resistance genes
One health
dc.subject.decs.pt_BR.fl_str_mv Genes MDR
Gene de Resistência a Multidrogas
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-06-27T12:22:45Z
dc.date.available.fl_str_mv 2021-06-27T12:22:45Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CARRAMASCHI, Isabel Nogueira et al. Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city. Acta Tropica, v. 220, 105962, 8 p, May 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47886
dc.identifier.issn.pt_BR.fl_str_mv 0001-706X
dc.identifier.doi.none.fl_str_mv 10.1016/j.actatropica.2021.105962
identifier_str_mv CARRAMASCHI, Isabel Nogueira et al. Surveillance of antimicrobial resistant bacteria in flies (Diptera) in Rio de Janeiro city. Acta Tropica, v. 220, 105962, 8 p, May 2021.
0001-706X
10.1016/j.actatropica.2021.105962
url https://www.arca.fiocruz.br/handle/icict/47886
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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