Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model

Detalhes bibliográficos
Autor(a) principal: Schwarz, Marcos Gustavo Araujo
Data de Publicação: 2021
Outros Autores: Antunes, Deborah, Brêda, Gabriela Coelho, Valente, Richard Hemmi, Freire, Denise Maria Guimarães
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/49755
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
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spelling Schwarz, Marcos Gustavo AraujoAntunes, DeborahBrêda, Gabriela CoelhoValente, Richard HemmiFreire, Denise Maria Guimarães2021-11-09T18:54:38Z2021-11-09T18:54:38Z2021SCHWARZ, Marcos Gustavo et al. Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model. Biomolecules, v. 11, 1486, p. 1-20, 2021.2218-273Xhttps://www.arca.fiocruz.br/handle/icict/4975510.3390/biom11101486engMDPIJatropha curcas L.SementeEsteraseDienelactona hidrolaseJatropha curcas L.SeedEsteraseDienelactone hydrolaseRevisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Microbiologia Molecular e Proteínas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Biotecnologia Microbiana. Rio de Janeiro, RJ, Brasil .Jatropha curcas contains seeds with a high oil content, suitable for biodiesel production. After oil extraction, the remaining mass can be a rich source of enzymes. However, data from the literature describing physicochemical characteristics for a monomeric esterase from the J. curcas seed did not fit the electrostatic catapult model for esterases/lipases. We decided to reevaluate this J. curcas esterase and extend its characterization to check this apparent discrepancy and gain insights into the enzyme’s potential as a biocatalyst. After anion exchange chromatography and two-dimensional gel electrophoresis, we identified the enzyme as belonging to the dienelactone hydrolase family, characterized by a cysteine as the nucleophile in the catalytic triad. The enzyme displayed a basic optimum hydrolysis pH of 9.0 and an acidic pI range, in contrast to literature data, making it well in line with the electrostatic catapult model. Furthermore, the enzyme showed low hydrolysis activity in an organic solvent-containing medium (isopropanol, acetonitrile, and ethanol), which reverted when recovering in an aqueous reaction mixture. This enzyme can be a valuable tool for hydrolysis reactions of short-chain esters, useful for pharmaceutical intermediates synthesis, due to both its high hydrolytic rate in basic pH and its stability in an organic solvent.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/49755/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcosSchwarz_DeboraAntunes_etal_IOC_2021.pdfMarcosSchwarz_DeboraAntunes_etal_IOC_2021.pdfapplication/pdf5491096https://www.arca.fiocruz.br/bitstream/icict/49755/2/MarcosSchwarz_DeboraAntunes_etal_IOC_2021.pdfa9fbfa9822b770d7a33cd59338e8a944MD52icict/497552021-11-17 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dc.title.pt_BR.fl_str_mv Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
title Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
spellingShingle Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
Schwarz, Marcos Gustavo Araujo
Jatropha curcas L.
Semente
Esterase
Dienelactona hidrolase
Jatropha curcas L.
Seed
Esterase
Dienelactone hydrolase
title_short Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
title_full Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
title_fullStr Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
title_full_unstemmed Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
title_sort Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
author Schwarz, Marcos Gustavo Araujo
author_facet Schwarz, Marcos Gustavo Araujo
Antunes, Deborah
Brêda, Gabriela Coelho
Valente, Richard Hemmi
Freire, Denise Maria Guimarães
author_role author
author2 Antunes, Deborah
Brêda, Gabriela Coelho
Valente, Richard Hemmi
Freire, Denise Maria Guimarães
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Schwarz, Marcos Gustavo Araujo
Antunes, Deborah
Brêda, Gabriela Coelho
Valente, Richard Hemmi
Freire, Denise Maria Guimarães
dc.subject.other.pt_BR.fl_str_mv Jatropha curcas L.
Semente
Esterase
Dienelactona hidrolase
topic Jatropha curcas L.
Semente
Esterase
Dienelactona hidrolase
Jatropha curcas L.
Seed
Esterase
Dienelactone hydrolase
dc.subject.en.pt_BR.fl_str_mv Jatropha curcas L.
Seed
Esterase
Dienelactone hydrolase
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-09T18:54:38Z
dc.date.available.fl_str_mv 2021-11-09T18:54:38Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SCHWARZ, Marcos Gustavo et al. Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model. Biomolecules, v. 11, 1486, p. 1-20, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49755
dc.identifier.issn.pt_BR.fl_str_mv 2218-273X
dc.identifier.doi.none.fl_str_mv 10.3390/biom11101486
identifier_str_mv SCHWARZ, Marcos Gustavo et al. Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model. Biomolecules, v. 11, 1486, p. 1-20, 2021.
2218-273X
10.3390/biom11101486
url https://www.arca.fiocruz.br/handle/icict/49755
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/49755/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/49755/2/MarcosSchwarz_DeboraAntunes_etal_IOC_2021.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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