Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP
| Main Author: | |
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| Publication Date: | 2011 |
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| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| Download full: | https://www.arca.fiocruz.br/handle/icict/7320 |
Summary: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, Brasil |
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Grassi, Maria Fernanda RiosGallazzi, Viviana Nilla OlavarriaKruschewsky, Ramon de AlmeidaMascarenhas, Rita Elizabeth MoreiraDourado, InêsCorreia, Luis Cláudio LemosCosta, Carlos Maurício de CastroCastro Filho, Bernardo Galvão2014-02-18T13:54:29Z2014-02-18T13:54:29Z2011GRASSI M. F. et al. Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP. Journal of Medical Virology, v. 83, n. 7, p. 1269-1274, 2011.1096-9071https://www.arca.fiocruz.br/handle/icict/732010.1002/jmv.22087engWiley-Liss, INCHuman T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSPinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, BrasilBahian School of Medicine and Public Health. EBMSP. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, BrasilFederal University of Bahia. Instituto de Saúde Coletiva. Salvador, BA, BrasilBahian School of Medicine and Public Health. EBMSP. Salvador, BA, BrasilFederal University of Ceará. Laboratory of Experimental Neurology and Neurophysiology. Fortaleza, CE, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, BrasilA high human T-cell lymphotropic virus type 1 (HTLV-1) proviral load is described in HTLV-1-associated diseases, especially HAM/TSP. However, the cut-off value to define high levels of HTLV-1 proviral load is not well established. 281 HTLV-1-infected patients from the HTLV reference center in Salvador, Brazil, were followed from 2005 to 2008. Patients were classified as asymptomatic, possible-, probable-, and definite-HAM/TSP, in accordance with diagnostic criteria proposed by De Castro-Costa et al. (2006): AIDS Res Hum Retroviruses 22:931-935. HTLV-1 proviral load was determined using real-time PCR. A receiver operator characteristic (ROC) curve was constructed using only asymptomatic individuals and definite-HAM/TSP patients. The ROC curve was used to predict the proviral load level that differentiates these two groups. Out of 281 patients, 189 were asymptomatic and 92 were diagnosed with HAM/TSP (22 possible, 23 probable, 47 definite). The mean HTLV-1 proviral load was higher in possible- (89,104 ± 93,006 copies/106 PBMC), -probable (175,854 ± 128,083 copies/106 PBMC), and definite-HAM/TSP patients (150,667 ± 122,320 copies/106 PBMC),when compared to asymptomatic individuals (27,178 ± 41,155 copies/106 PBMC) (P < 0.0001). A comparison of all HAM/TSP groups showed the highest proviral loads in probable-HAM/TSP patients, yet the differences in mean values were not statistically significant. The ROC curve suggested a value of 49,865 copies/106 PBMC, with 87% sensitivity (95% CI » 74-95) and 81% specificity (95% CI » 75-86), as the best proviral load cut-off point to differentiate definite HAM/TSP patients from asymptomatic individuals. HTLV-1 proviral loads are higher in groups of infected patients with eurological symptoms and may represent a relevant biological marker of disease progression.HTLV-1Proviral loadBiomarkerHAM/TSPBrazilInfecções por HTLV-I/diagnósticoInfecções por HTLV-I/virologiaVírus 1 Linfotrópico T Humano/crescimento & desenvolvimentoParaparesia Espástica Tropical/diagnósticoParaparesia Espástica Tropical/virologiaProvirus/crescimento & desenvolvimentoDoenças da Medula Espinal/diagnósticoDoenças da Medula Espinal/virologiaCarga ViralAdolescenteAdultoIdosoIdoso de 80 Anos ou maisDoenças AssintomáticasMarcadores BiológicosBrasilCriançaDNA Viral/análiseProgressão da DoençaFemininoInfecções por HTLV-I/complicaçõesInfecções por HTLV-I/patologiaVírus 1 Linfotrópico T Humano/genéticaLeucócitos Mononucleares/patologiaHumanosParaparesia Espástica Tropical/complicaçõesReação em Cadeia da PolimeraseValor Preditivo dos TestesProvirus/genéticaDoenças da Medula Espinal/complicaçõesDoenças da Medula Espinal/patologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGrassi, MF. et al. Human T cell. ..J Med. Virol. 2011, v. 83, p.1269.pdfGrassi, MF. et al. Human T cell. ..J Med. Virol. 2011, v. 83, p.1269.pdfapplication/pdf92286https://www.arca.fiocruz.br/bitstream/icict/7320/1/Grassi%2c%20MF.%20et%20al.%20Human%20T%20cell.%20..J%20Med.%20Virol.%202011%2c%20v.%2083%2c%20p.1269.pdfa51c39b8e4ac70fbcf448eb8f6f987b9MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7320/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTGrassi, MF. et al. Human T cell. ..J Med. Virol. 2011, v. 83, p.1269.pdf.txtGrassi, MF. et al. Human T cell. ..J Med. Virol. 2011, v. 83, p.1269.pdf.txtExtracted texttext/plain29409https://www.arca.fiocruz.br/bitstream/icict/7320/5/Grassi%2c%20MF.%20et%20al.%20Human%20T%20cell.%20..J%20Med.%20Virol.%202011%2c%20v.%2083%2c%20p.1269.pdf.txtd99b707814f537a076baa33ba0bc1d21MD55THUMBNAILGrassi, MF. et al. Human T cell. ..J Med. Virol. 2011, v. 83, p.1269.pdf.jpgGrassi, MF. et al. Human T cell. ..J Med. 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| dc.title.pt_BR.fl_str_mv |
Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP |
| title |
Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP |
| spellingShingle |
Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP Grassi, Maria Fernanda Rios HTLV-1 Proviral load Biomarker HAM/TSP Brazil Infecções por HTLV-I/diagnóstico Infecções por HTLV-I/virologia Vírus 1 Linfotrópico T Humano/crescimento & desenvolvimento Paraparesia Espástica Tropical/diagnóstico Paraparesia Espástica Tropical/virologia Provirus/crescimento & desenvolvimento Doenças da Medula Espinal/diagnóstico Doenças da Medula Espinal/virologia Carga Viral Adolescente Adulto Idoso Idoso de 80 Anos ou mais Doenças Assintomáticas Marcadores Biológicos Brasil Criança DNA Viral/análise Progressão da Doença Feminino Infecções por HTLV-I/complicações Infecções por HTLV-I/patologia Vírus 1 Linfotrópico T Humano/genética Leucócitos Mononucleares/patologia Humanos Paraparesia Espástica Tropical/complicações Reação em Cadeia da Polimerase Valor Preditivo dos Testes Provirus/genética Doenças da Medula Espinal/complicações Doenças da Medula Espinal/patologia |
| title_short |
Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP |
| title_full |
Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP |
| title_fullStr |
Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP |
| title_full_unstemmed |
Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP |
| title_sort |
Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP |
| author |
Grassi, Maria Fernanda Rios |
| author_facet |
Grassi, Maria Fernanda Rios Gallazzi, Viviana Nilla Olavarria Kruschewsky, Ramon de Almeida Mascarenhas, Rita Elizabeth Moreira Dourado, Inês Correia, Luis Cláudio Lemos Costa, Carlos Maurício de Castro Castro Filho, Bernardo Galvão |
| author_role |
author |
| author2 |
Gallazzi, Viviana Nilla Olavarria Kruschewsky, Ramon de Almeida Mascarenhas, Rita Elizabeth Moreira Dourado, Inês Correia, Luis Cláudio Lemos Costa, Carlos Maurício de Castro Castro Filho, Bernardo Galvão |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Grassi, Maria Fernanda Rios Gallazzi, Viviana Nilla Olavarria Kruschewsky, Ramon de Almeida Mascarenhas, Rita Elizabeth Moreira Dourado, Inês Correia, Luis Cláudio Lemos Costa, Carlos Maurício de Castro Castro Filho, Bernardo Galvão |
| dc.subject.en.pt_BR.fl_str_mv |
HTLV-1 Proviral load Biomarker HAM/TSP Brazil |
| topic |
HTLV-1 Proviral load Biomarker HAM/TSP Brazil Infecções por HTLV-I/diagnóstico Infecções por HTLV-I/virologia Vírus 1 Linfotrópico T Humano/crescimento & desenvolvimento Paraparesia Espástica Tropical/diagnóstico Paraparesia Espástica Tropical/virologia Provirus/crescimento & desenvolvimento Doenças da Medula Espinal/diagnóstico Doenças da Medula Espinal/virologia Carga Viral Adolescente Adulto Idoso Idoso de 80 Anos ou mais Doenças Assintomáticas Marcadores Biológicos Brasil Criança DNA Viral/análise Progressão da Doença Feminino Infecções por HTLV-I/complicações Infecções por HTLV-I/patologia Vírus 1 Linfotrópico T Humano/genética Leucócitos Mononucleares/patologia Humanos Paraparesia Espástica Tropical/complicações Reação em Cadeia da Polimerase Valor Preditivo dos Testes Provirus/genética Doenças da Medula Espinal/complicações Doenças da Medula Espinal/patologia |
| dc.subject.decs.pt_BR.fl_str_mv |
Infecções por HTLV-I/diagnóstico Infecções por HTLV-I/virologia Vírus 1 Linfotrópico T Humano/crescimento & desenvolvimento Paraparesia Espástica Tropical/diagnóstico Paraparesia Espástica Tropical/virologia Provirus/crescimento & desenvolvimento Doenças da Medula Espinal/diagnóstico Doenças da Medula Espinal/virologia Carga Viral Adolescente Adulto Idoso Idoso de 80 Anos ou mais Doenças Assintomáticas Marcadores Biológicos Brasil Criança DNA Viral/análise Progressão da Doença Feminino Infecções por HTLV-I/complicações Infecções por HTLV-I/patologia Vírus 1 Linfotrópico T Humano/genética Leucócitos Mononucleares/patologia Humanos Paraparesia Espástica Tropical/complicações Reação em Cadeia da Polimerase Valor Preditivo dos Testes Provirus/genética Doenças da Medula Espinal/complicações Doenças da Medula Espinal/patologia |
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Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, Brasil |
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2011 |
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2011 |
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2014-02-18T13:54:29Z |
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2014-02-18T13:54:29Z |
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GRASSI M. F. et al. Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP. Journal of Medical Virology, v. 83, n. 7, p. 1269-1274, 2011. |
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https://www.arca.fiocruz.br/handle/icict/7320 |
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1096-9071 |
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10.1002/jmv.22087 |
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GRASSI M. F. et al. Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP. Journal of Medical Virology, v. 83, n. 7, p. 1269-1274, 2011. 1096-9071 10.1002/jmv.22087 |
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Wiley-Liss, INC |
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