Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Campos, Taís Menezes Cerqueira
Data de Publicação: 2014
Outros Autores: Passos, Sara Timoteo, Novais, Fernanda Oliveira, Beiting, Daniel P, Costa, Rúbia Suely Santana, Queiroz, Adriano, Mosser, David, Scott, Phillip, Carvalho, Edgar Marcelino de, Carvalho, Lucas Pedreira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24899
Resumo: CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Campos, Taís Menezes CerqueiraPassos, Sara TimoteoNovais, Fernanda OliveiraBeiting, Daniel PCosta, Rúbia Suely SantanaQueiroz, AdrianoMosser, DavidScott, PhillipCarvalho, Edgar Marcelino deCarvalho, Lucas Pedreira de2018-02-21T17:09:59Z2018-02-21T17:09:59Z2014CAMPOS, T. M. C. et al. Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, v. 8, n. 11, e3282, 2014.1935-2727https://www.arca.fiocruz.br/handle/icict/2489910.1371/journal.pntd.0003282CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.National Institutes of Health [grant AI088650] and Conselho Nacional de Pesquisa Instituto Nacional de Ciência e Tecnologia – Doenças Tropicais [grant 573839/2008-5].Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, BrazilUniversity of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversity of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, BrasilUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Pennsylvania. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, BrazilUniversidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciências e Tecnologia. Doenças Tropicais. Salvador, BA, Brazil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilCutaneous leishmaniasis (CL) due to L.braziliensis infection is characterized by a strong inflammatory response with high levels of TNF and ulcer development. Less attention has been given to the role of mononuclear phagocytes to this process. Monocytes constitute a heterogeneous population subdivided into classical, intermediate and non-classical, and are known to migrate to inflammatory sites and secrete inflammatory mediators. TNF participates in the induction of matrix metalloproteinases (MMPs). MMP-9 is an enzyme that degrades basal membrane and its activity is controlled by the tissue inhibitor of metalloproteinase. Methods: Mononuclear cells were obtained from ex-vivo labeling sub-populations of monocytes and MMP-9, and the frequency was determined by flow cytometry. Culture was performed during 72 hours, stimulating the cells with SLA, levels of MMP-9 and TIMP-1 in the supernatants were determined by ELISA. Results: We observed that cells from CL lesions secrete high amounts of MMP-9 when compared to healthy subjects. Although MMP-9 was produced by monocytes, non-classical ones were the main source of this enzyme. We also observed that TNF produced in high level during CL contributes to MMP-9 production. Conclusions: These observations emphasize the role of monocytes, TNF and MMP-9 in the pathogenesis of L. braziliensis infection.engPublic Library of ScienceLeishmaniose CutâneaMonócitosFator de necrose tumoral alfaLeucócitos mononuclearesMatrix Metalloproteinase 9HumanosLeishmaniasis, CutaneousMonocytesTumor Necrosis Factor-alphaLeukocytes, MononuclearMatrix Metalloproteinase 9HumansMatrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24899/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCampos TM Matrix Metalloproteinase 9 Production....pdfCampos TM Matrix Metalloproteinase 9 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dc.title.pt_BR.fl_str_mv Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis
title Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis
spellingShingle Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis
Campos, Taís Menezes Cerqueira
Leishmaniose Cutânea
Monócitos
Fator de necrose tumoral alfa
Leucócitos mononucleares
Matrix Metalloproteinase 9
Humanos
Leishmaniasis, Cutaneous
Monocytes
Tumor Necrosis Factor-alpha
Leukocytes, Mononuclear
Matrix Metalloproteinase 9
Humans
title_short Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis
title_full Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis
title_fullStr Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis
title_full_unstemmed Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis
title_sort Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis
author Campos, Taís Menezes Cerqueira
author_facet Campos, Taís Menezes Cerqueira
Passos, Sara Timoteo
Novais, Fernanda Oliveira
Beiting, Daniel P
Costa, Rúbia Suely Santana
Queiroz, Adriano
Mosser, David
Scott, Phillip
Carvalho, Edgar Marcelino de
Carvalho, Lucas Pedreira de
author_role author
author2 Passos, Sara Timoteo
Novais, Fernanda Oliveira
Beiting, Daniel P
Costa, Rúbia Suely Santana
Queiroz, Adriano
Mosser, David
Scott, Phillip
Carvalho, Edgar Marcelino de
Carvalho, Lucas Pedreira de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Campos, Taís Menezes Cerqueira
Passos, Sara Timoteo
Novais, Fernanda Oliveira
Beiting, Daniel P
Costa, Rúbia Suely Santana
Queiroz, Adriano
Mosser, David
Scott, Phillip
Carvalho, Edgar Marcelino de
Carvalho, Lucas Pedreira de
dc.subject.other.pt_BR.fl_str_mv Leishmaniose Cutânea
Monócitos
Fator de necrose tumoral alfa
Leucócitos mononucleares
Matrix Metalloproteinase 9
Humanos
topic Leishmaniose Cutânea
Monócitos
Fator de necrose tumoral alfa
Leucócitos mononucleares
Matrix Metalloproteinase 9
Humanos
Leishmaniasis, Cutaneous
Monocytes
Tumor Necrosis Factor-alpha
Leukocytes, Mononuclear
Matrix Metalloproteinase 9
Humans
dc.subject.en.pt_BR.fl_str_mv Leishmaniasis, Cutaneous
Monocytes
Tumor Necrosis Factor-alpha
Leukocytes, Mononuclear
Matrix Metalloproteinase 9
Humans
description CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2018-02-21T17:09:59Z
dc.date.available.fl_str_mv 2018-02-21T17:09:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CAMPOS, T. M. C. et al. Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, v. 8, n. 11, e3282, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24899
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0003282
identifier_str_mv CAMPOS, T. M. C. et al. Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. PLoS Neglected Tropical Diseases, v. 8, n. 11, e3282, 2014.
1935-2727
10.1371/journal.pntd.0003282
url https://www.arca.fiocruz.br/handle/icict/24899
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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