Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil

Detalhes bibliográficos
Autor(a) principal: Vinhas, Solange Alves
Data de Publicação: 2013
Outros Autores: Palaci, Moisés, Marques, Hebert Silva, Aguiar, Paola Poloni Lobo de, Ribeiro, Fabíola Karla, Peres, Renata Lyrio, Dietze, Reynaldo, Gomes, Harrison Magdinier, Suffys, Phillip Noel, Golub, Jonathan E., Riley, Lee W., Maciel, Ethel Leonor Noia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11578
Resumo: Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
id CRUZ_8ed05916bfec73dd78f8bd38991e8e85
oai_identifier_str oai:www.arca.fiocruz.br:icict/11578
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Vinhas, Solange AlvesPalaci, MoisésMarques, Hebert SilvaAguiar, Paola Poloni Lobo deRibeiro, Fabíola KarlaPeres, Renata LyrioDietze, ReynaldoGomes, Harrison MagdinierSuffys, Phillip NoelGolub, Jonathan E.Riley, Lee W.Maciel, Ethel Leonor Noia2015-08-19T13:49:35Z2015-08-19T13:49:35Z2013VINHAS, Solange Alves; Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil. Tuberculosis (Edinb). v.93, n.2, p.207–212, 2013.1472-9792https://www.arca.fiocruz.br/handle/icict/1157810.1016/j.tube.2012.09.001engScienceDirectMycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Johns Hopkins University. School of Medicine. Baltimore, MD, USA.University of California. School of Public Health. Division of Infectious Disease and Vaccinology. Berkeley, CA, USA.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. bPrograma de Pós-graduação em Saúde Coletiva. Vitória, ES, Brasil.Mycobacterium tuberculosis (Mtb) strains designated as RDRio are responsible for a large cluster of new cases of tuberculosis (TB) in Rio de Janeiro. They were previously shown to be associated with severe manifestations of TB. Here, we used three genotyping methods (IS6110 RFLP, spoligotyping, and multiplex PCR) to characterize RDRio and non-RDRio strains from the metropolitan area of Vitória, State of Espirito Santo in southeast Brazil to determine strain diversity and transmission patterns. Strains with identical IS6110 RFLP patterns were considered to belong to a cluster indicative of recent transmission. Between 2000 and 2010, we identified 5470 new TB patients and genotyped 981 Mtb strains. Of these, 376 (38%) were RDRio. By RFLP, 180 (48%) of 376 RDRio strains and 235 (40%) of 593 non-RDRio strains belonged to RFLP cluster pattern groups (p = 0.023). Simpson’s diversity index based on RFLP patterns was 0.96 for RDRio and 0.98 for non-RDRio strains. Thus, although RDRio strains appear to be comprised of a fewer number of RFLP genotypes, they represent a heterogeneous group. While TB cases caused by RDRio appear more likely to be due to recent transmission than cases caused by non-RDRio strains, the difference is small. These observations suggest that factors other than inherent biological characteristic of RDRio lineages are more important in determining recent transmission, and that public health measures to interrupt new transmissions need to be emphasized for TB control in Vitória.TuberculosisDNA fingerprintingMycobacterium tuberculosisEpidemiologic surveillanceTuberculoseMycobacterium tuberculosisImpressões Digitais de DNAVigilância Epidemiológicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11578/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALphillip_suffys2_etal_IOC_2013.pdfapplication/pdf1588404https://www.arca.fiocruz.br/bitstream/icict/11578/2/phillip_suffys2_etal_IOC_2013.pdf11bc6ace17b6144b5a042e2b0195b473MD52TEXTphillip_suffys2_etal_IOC_2013.pdf.txtphillip_suffys2_etal_IOC_2013.pdf.txtExtracted texttext/plain32509https://www.arca.fiocruz.br/bitstream/icict/11578/3/phillip_suffys2_etal_IOC_2013.pdf.txtb03d0cc5cc1270cc12038db65a12bdd2MD53icict/115782022-06-24 13:12:37.759oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:12:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil
title Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil
spellingShingle Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil
Vinhas, Solange Alves
Tuberculosis
DNA fingerprinting
Mycobacterium tuberculosis
Epidemiologic surveillance
Tuberculose
Mycobacterium tuberculosis
Impressões Digitais de DNA
Vigilância Epidemiológica
title_short Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil
title_full Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil
title_fullStr Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil
title_full_unstemmed Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil
title_sort Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil
author Vinhas, Solange Alves
author_facet Vinhas, Solange Alves
Palaci, Moisés
Marques, Hebert Silva
Aguiar, Paola Poloni Lobo de
Ribeiro, Fabíola Karla
Peres, Renata Lyrio
Dietze, Reynaldo
Gomes, Harrison Magdinier
Suffys, Phillip Noel
Golub, Jonathan E.
Riley, Lee W.
Maciel, Ethel Leonor Noia
author_role author
author2 Palaci, Moisés
Marques, Hebert Silva
Aguiar, Paola Poloni Lobo de
Ribeiro, Fabíola Karla
Peres, Renata Lyrio
Dietze, Reynaldo
Gomes, Harrison Magdinier
Suffys, Phillip Noel
Golub, Jonathan E.
Riley, Lee W.
Maciel, Ethel Leonor Noia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vinhas, Solange Alves
Palaci, Moisés
Marques, Hebert Silva
Aguiar, Paola Poloni Lobo de
Ribeiro, Fabíola Karla
Peres, Renata Lyrio
Dietze, Reynaldo
Gomes, Harrison Magdinier
Suffys, Phillip Noel
Golub, Jonathan E.
Riley, Lee W.
Maciel, Ethel Leonor Noia
dc.subject.en.pt_BR.fl_str_mv Tuberculosis
DNA fingerprinting
Mycobacterium tuberculosis
Epidemiologic surveillance
topic Tuberculosis
DNA fingerprinting
Mycobacterium tuberculosis
Epidemiologic surveillance
Tuberculose
Mycobacterium tuberculosis
Impressões Digitais de DNA
Vigilância Epidemiológica
dc.subject.decs.pt_BR.fl_str_mv Tuberculose
Mycobacterium tuberculosis
Impressões Digitais de DNA
Vigilância Epidemiológica
description Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:35Z
dc.date.available.fl_str_mv 2015-08-19T13:49:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VINHAS, Solange Alves; Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil. Tuberculosis (Edinb). v.93, n.2, p.207–212, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11578
dc.identifier.issn.pt_BR.fl_str_mv 1472-9792
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.tube.2012.09.001
identifier_str_mv VINHAS, Solange Alves; Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil. Tuberculosis (Edinb). v.93, n.2, p.207–212, 2013.
1472-9792
10.1016/j.tube.2012.09.001
url https://www.arca.fiocruz.br/handle/icict/11578
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ScienceDirect
publisher.none.fl_str_mv ScienceDirect
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/11578/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/11578/2/phillip_suffys2_etal_IOC_2013.pdf
https://www.arca.fiocruz.br/bitstream/icict/11578/3/phillip_suffys2_etal_IOC_2013.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
11bc6ace17b6144b5a042e2b0195b473
b03d0cc5cc1270cc12038db65a12bdd2
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324850818809856

Registros relacionados