Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro

Detalhes bibliográficos
Autor(a) principal: Brodskyn, Claudia Ida
Data de Publicação: 2001
Outros Autores: DeKrey, G. K, Titus, R. G
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20420
Resumo: Brodskyn, Claudia Ida “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Brodskyn, Claudia IdaDeKrey, G. KTitus, R. G2017-07-26T19:12:32Z2017-07-26T19:12:32Z2001BRODSKYN, C. I.; DEKREY, G. K.; TITUS, R. G. Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro. Infection and Immunity, v. 69, n. 2, p. 665-672, 2001.0019-9567https://www.arca.fiocruz.br/handle/icict/2042010.1128/IAI.69.2.665-672.2001Brodskyn, Claudia Ida “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.NIH grant AI-29955 and a fellowship (to C.I.B.) from CAPES/Fulbright (1997) and CAPES-Brasilia Brazil (1998).Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunologia. Departamento de Biointeração. Salvador, BA, BrasilColorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Pathology. Fort Collins, ColoradoColorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Pathology. Fort Collins, ColoradoThe importance of CD40, CD80, and CD86 costimulatory molecules in anti-Leishmania immune responses has been established in murine models. A role for these costimulatory molecules in human anti-Leishmania immune responses was investigated in this study. Autologous macrophages and peripheral blood leukocytes (PBL) were prepared from peripheral blood mononuclear cells of Leishmania-naive donors and cultured with or without Leishmania major in various combinations. After 7 days of culture, high levels of CD40 and CD86 were expressed on macrophages in the presence or absence of L. major. When macrophages were cultured for an additional 7 days with PBL, expression of all three costimulatory molecules was detected. When L. major was present in these cultures, the expression of CD80, and to a lesser extent CD40, on macrophages was enhanced. Blockade of CD80, CD86, or both molecules (in the order of greatest effect) in cultures containing macrophages, PBL, and L. major significantly inhibited the production of gamma interferon, interleukin-5 (IL-5), and IL-12. Blockade of CD40-CD154 interactions also significantly inhibited production of these cytokines in response to L. major. Production of IL-10 was unaltered by the blockade of these costimulatory molecules. Thus, these data suggest that CD40, CD80, and CD86 expression and regulation may significantly impact anti-Leishmania immune responses in humans.engAmerican Society for MicrobiologyLeishmaniaLeishmania majorMoleculasCelulasHumanosLeishmaniaLeishmania majorMoleculesCellsHumansInfluence of costimulatory molecules on immune response to Leishmania major by human cells in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20420/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBrodskyn CI influence of costumulatory .....pdfBrodskyn CI influence of costumulatory 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dc.title.pt_BR.fl_str_mv Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro
title Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro
spellingShingle Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro
Brodskyn, Claudia Ida
Leishmania
Leishmania major
Moleculas
Celulas
Humanos
Leishmania
Leishmania major
Molecules
Cells
Humans
title_short Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro
title_full Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro
title_fullStr Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro
title_full_unstemmed Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro
title_sort Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro
author Brodskyn, Claudia Ida
author_facet Brodskyn, Claudia Ida
DeKrey, G. K
Titus, R. G
author_role author
author2 DeKrey, G. K
Titus, R. G
author2_role author
author
dc.contributor.author.fl_str_mv Brodskyn, Claudia Ida
DeKrey, G. K
Titus, R. G
dc.subject.other.pt_BR.fl_str_mv Leishmania
Leishmania major
Moleculas
Celulas
Humanos
topic Leishmania
Leishmania major
Moleculas
Celulas
Humanos
Leishmania
Leishmania major
Molecules
Cells
Humans
dc.subject.en.pt_BR.fl_str_mv Leishmania
Leishmania major
Molecules
Cells
Humans
description Brodskyn, Claudia Ida “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2017-07-26T19:12:32Z
dc.date.available.fl_str_mv 2017-07-26T19:12:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRODSKYN, C. I.; DEKREY, G. K.; TITUS, R. G. Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro. Infection and Immunity, v. 69, n. 2, p. 665-672, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20420
dc.identifier.issn.pt_BR.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.69.2.665-672.2001
identifier_str_mv BRODSKYN, C. I.; DEKREY, G. K.; TITUS, R. G. Influence of costimulatory molecules on immune response to Leishmania major by human cells in vitro. Infection and Immunity, v. 69, n. 2, p. 665-672, 2001.
0019-9567
10.1128/IAI.69.2.665-672.2001
url https://www.arca.fiocruz.br/handle/icict/20420
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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