Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells

Detalhes bibliográficos
Autor(a) principal: Oliveira, Martha M.
Data de Publicação: 2001
Outros Autores: Charlab, Rosane, Pessolani, Maria Cristina V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37519
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
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spelling Oliveira, Martha M.Charlab, RosanePessolani, Maria Cristina V.2019-12-02T13:29:44Z2019-12-02T13:29:44Z2001OLIVEIRA, Martha M.; CHARLAB, Rosane; PESSOLANI, Maria Cristina V. Mycobacterium bovis BCG but not Mycobacterium leprae Induces TNF-a Secretion in Human Monocytic THP-1 Cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 7, p. 973-978, Oct. 2001.0074-0276https://www.arca.fiocruz.br/handle/icict/3751910.1590/s0074-027620010007000151678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Mycobacterium lepraeBCGTHP-1TNF-AlphaMycobacterium lepraeBCGTNF-AlphaTHP-1Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Centro Brasileito de Pesquisas Físicas. CNPq. Rio de Janeiro, RJ, Brasil. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.In this study, we compared the level of TNF-alpha secretion induced in monocytic THP-1 cells after phagocytosis of Mycobacterium leprae, the causative agent of leprosy, and M. bovis BCG, an attenuated strain used as a vaccine against leprosy and tuberculosis. The presence of M. leprae and BCG was observed in more than 80% of the cells after 24 h of exposure. However, BCG but not M. leprae was able to induce TNF-alpha secretion in these cells. Moreover, THP-1 cells treated simultaneously with BCG and M. leprae secreted lower levels of TNF-alpha compared to cells incubated with BCG alone. M. leprae was able, however, to induce TNF-alpha secretion both in blood-derived monocytes as well as in THP-1 cells pretreated with phorbol myristate acetate. The inclusion of streptomycin in our cultures, together with the fact that the use of both gamma-irradiated M. leprae and heat-killed BCG gave similar results, indicate that the differences observed were not due to differences in viability but in intrinsic properties between M. leprae and BCG. These data suggest that the capacity of M. leprae to induce TNF-alpha is dependent on the stage of cell maturation and emphasize the potential of this model to explore differences in the effects triggered by vaccine strain versus pathogenic species of mycobacteria on the host cell physiology and metabolism.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37519/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariaCV_Pessolani_etal_IOC_2001.pdfMariaCV_Pessolani_etal_IOC_2001.pdfapplication/pdf62944https://www.arca.fiocruz.br/bitstream/icict/37519/2/MariaCV_Pessolani_etal_IOC_2001.pdf3298c6378964170f3f36c27c505f2550MD52TEXTMariaCV_Pessolani_etal_IOC_2001.pdf.txtMariaCV_Pessolani_etal_IOC_2001.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells
title Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells
spellingShingle Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells
Oliveira, Martha M.
Mycobacterium leprae
BCG
THP-1
TNF-Alpha
Mycobacterium leprae
BCG
TNF-Alpha
THP-1
title_short Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells
title_full Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells
title_fullStr Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells
title_full_unstemmed Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells
title_sort Mycobacterium bovis BCG but not Mycobacterium leprae induces TNF-alpha secretion in human monocytic THP-1 cells
author Oliveira, Martha M.
author_facet Oliveira, Martha M.
Charlab, Rosane
Pessolani, Maria Cristina V.
author_role author
author2 Charlab, Rosane
Pessolani, Maria Cristina V.
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira, Martha M.
Charlab, Rosane
Pessolani, Maria Cristina V.
dc.subject.other.pt_BR.fl_str_mv Mycobacterium leprae
BCG
THP-1
TNF-Alpha
topic Mycobacterium leprae
BCG
THP-1
TNF-Alpha
Mycobacterium leprae
BCG
TNF-Alpha
THP-1
dc.subject.en.pt_BR.fl_str_mv Mycobacterium leprae
BCG
TNF-Alpha
THP-1
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2019-12-02T13:29:44Z
dc.date.available.fl_str_mv 2019-12-02T13:29:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Martha M.; CHARLAB, Rosane; PESSOLANI, Maria Cristina V. Mycobacterium bovis BCG but not Mycobacterium leprae Induces TNF-a Secretion in Human Monocytic THP-1 Cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 7, p. 973-978, Oct. 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37519
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762001000700015
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv OLIVEIRA, Martha M.; CHARLAB, Rosane; PESSOLANI, Maria Cristina V. Mycobacterium bovis BCG but not Mycobacterium leprae Induces TNF-a Secretion in Human Monocytic THP-1 Cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 7, p. 973-978, Oct. 2001.
0074-0276
10.1590/s0074-02762001000700015
1678-8060
url https://www.arca.fiocruz.br/handle/icict/37519
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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