Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV

Detalhes bibliográficos
Autor(a) principal: Abreu, Ana Paula de
Data de Publicação: 2021
Outros Autores: Teston, Ana Paula Margioto, Massago, Miyoko, Silva, Hevillyn Fernanda Lucas da, Ferreira, Érika Cristina, Fernandes, Nilma de Souza, Araújo, Silvana Marques de, Dale, Carolina, Galvão, Cleber, Toledo, Max Jean de Ornelas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56296
Resumo: Universidade Estadual de Maringá (UEM). Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências da Saúde. Maringá, PR, Brasil.
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spelling Abreu, Ana Paula deTeston, Ana Paula MargiotoMassago, MiyokoSilva, Hevillyn Fernanda Lucas daFerreira, Érika CristinaFernandes, Nilma de SouzaAraújo, Silvana Marques deDale, CarolinaGalvão, CleberToledo, Max Jean de Ornelas2023-01-03T18:18:44Z2023-01-03T18:18:44Z2021ABREU, Ana Paula de et al. Experimental infection of Rhodnius robustus Larrousse, 1 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV 4. p. 1 - 27,https://www.arca.fiocruz.br/handle/icict/56296engScience DirectDoença de ChagasVetoresParasitaSuscetibilidade à infecçãoInteraçãoCompetência vetorialChagas diseaseVectorsParasiteInteractionSusceptibility to infectionVector competenceExperimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IVinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Maringá (UEM). Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências da Saúde. Maringá, PR, Brasil.Universidade Estadual de Maringá (UEM). Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências da Saúde. Maringá, PR, Brasil / Centro Universitário Uningá. Departamento de Farmácia. Maringá, PR, Brasil.UEM. Centro de Ciências Biológicas. Programa de Pós-Graduação em Ciências Biológicas. Maringá, PR, Brasil.Universidade Estadual de Maringá (UEM). Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências da Saúde. Maringá, PR, Brasil.Universidade Estadual de Maringá (UEM). Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências da Saúde. Maringá, PR, Brasil.Centro de Ciências Biológicas, UEM. Programa de Pós-Graduação em Ciências Biológicas. Maringá, PR, Brasil.Universidade Estadual de Maringá (UEM). Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências da Saúde. Maringá, PR, Brasil /Departamento de Ciências Básicas da Saúde, CCS, UEM. Maringá, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biodiversidade Entomológica. Coleção Entomológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.Universidade Estadual de Maringá (UEM). Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências da Saúde. Maringá, PR, Brasil / Centro Universitário Uningá. Departamento de Farmácia. Maringá, PR, Brasil / Departamento de Ciências Básicas da Saúde, CCS, UEM. Maringá, PR, Brasil.Vector competence of triatomines (kissing bugs) for Trypanosoma cruzi transmission depends on the parasite-vector interaction and the genetic constitution of both. This study evaluates the susceptibility and vector competence of Rhodnius robustus experimentally infected with T. cruzi IV (TcIV). Nymphs were fed on infected mice or an artificial feeder with blood containing culture-derived metacyclic trypomastigotes (CMT) or blood trypomastigotes (BT). The intestinal contents (IC) and excreta of the insects were examined by fresh examination and kDNA-PCR. The rate of metacyclogenesis was also determined by differential counts. Fifth instar nymphs fed with CMT ingested a greater blood volume (mean of 74.5 μL) and a greater amount of parasites (mean of 149,000 CMT/μL), and had higher positivity in the fresh examination of the IC. Third instar nymphs fed with CMT had higher positivity (33.3%) in the fresh examination of the excreta. On the 20th day after infection (dai), infective metacyclic trypomastigote (MT) forms were predominant in the excreta of 3/4 experimental groups, and on the 30th dai, the different parasitic forms were observed in the IC of all the groups. Higher percentages of MT were observed in the excreta of the 5th instar nymphs group (84.1%) and in the IC of the 3rd instar nymphs group (80.0%). Rhodnius robustus presented high susceptibility to infection since all nymphs were infected, regardless of the method used for blood meal, in addition these insects demonstrated vector competence for TcIV with high rates of metacyclogenesis being evident.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56296/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCleberGalvao_AnaPaulaAbreu_etal_IOC_2022.pdfCleberGalvao_AnaPaulaAbreu_etal_IOC_2022.pdfapplication/pdf548754https://www.arca.fiocruz.br/bitstream/icict/56296/2/CleberGalvao_AnaPaulaAbreu_etal_IOC_2022.pdfed885bc5aa3b08d94233aa7cf8411083MD52icict/562962023-01-03 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dc.title.en_US.fl_str_mv Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV
title Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV
spellingShingle Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV
Abreu, Ana Paula de
Doença de Chagas
Vetores
Parasita
Suscetibilidade à infecção
Interação
Competência vetorial
Chagas disease
Vectors
Parasite
Interaction
Susceptibility to infection
Vector competence
title_short Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV
title_full Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV
title_fullStr Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV
title_full_unstemmed Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV
title_sort Experimental infection of Rhodnius robustus Larrousse, 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV
author Abreu, Ana Paula de
author_facet Abreu, Ana Paula de
Teston, Ana Paula Margioto
Massago, Miyoko
Silva, Hevillyn Fernanda Lucas da
Ferreira, Érika Cristina
Fernandes, Nilma de Souza
Araújo, Silvana Marques de
Dale, Carolina
Galvão, Cleber
Toledo, Max Jean de Ornelas
author_role author
author2 Teston, Ana Paula Margioto
Massago, Miyoko
Silva, Hevillyn Fernanda Lucas da
Ferreira, Érika Cristina
Fernandes, Nilma de Souza
Araújo, Silvana Marques de
Dale, Carolina
Galvão, Cleber
Toledo, Max Jean de Ornelas
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Abreu, Ana Paula de
Teston, Ana Paula Margioto
Massago, Miyoko
Silva, Hevillyn Fernanda Lucas da
Ferreira, Érika Cristina
Fernandes, Nilma de Souza
Araújo, Silvana Marques de
Dale, Carolina
Galvão, Cleber
Toledo, Max Jean de Ornelas
dc.subject.other.en_US.fl_str_mv Doença de Chagas
Vetores
Parasita
Suscetibilidade à infecção
Interação
Competência vetorial
topic Doença de Chagas
Vetores
Parasita
Suscetibilidade à infecção
Interação
Competência vetorial
Chagas disease
Vectors
Parasite
Interaction
Susceptibility to infection
Vector competence
dc.subject.en.en_US.fl_str_mv Chagas disease
Vectors
Parasite
Interaction
Susceptibility to infection
Vector competence
description Universidade Estadual de Maringá (UEM). Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências da Saúde. Maringá, PR, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2023-01-03T18:18:44Z
dc.date.available.fl_str_mv 2023-01-03T18:18:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ABREU, Ana Paula de et al. Experimental infection of Rhodnius robustus Larrousse, 1 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV 4. p. 1 - 27,
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56296
identifier_str_mv ABREU, Ana Paula de et al. Experimental infection of Rhodnius robustus Larrousse, 1 1927 (Hemiptera, Reduviidae, Triatominae) with Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) IV 4. p. 1 - 27,
url https://www.arca.fiocruz.br/handle/icict/56296
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Science Direct
publisher.none.fl_str_mv Science Direct
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56296/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/56296/2/CleberGalvao_AnaPaulaAbreu_etal_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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