Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis

Detalhes bibliográficos
Autor(a) principal: Almeida, Marcos Abreu
Data de Publicação: 2020
Outros Autores: Almeida-Paes, Rodrigo, Guimarães, Allan Jefferson, Valente, Richard Hemmi, Soares, Célia Maria de Almeida, Zancopé-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44918
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Almeida, Marcos AbreuAlmeida-Paes, RodrigoGuimarães, Allan JeffersonValente, Richard HemmiSoares, Célia Maria de AlmeidaZancopé-Oliveira, Rosely Maria2020-12-15T19:26:34Z2020-12-15T19:26:34Z2020ALMEIDA, Marcos Abreu et al. Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens-Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis. Frontiers in Cellular and Infection Microbiology, v. 10, p. 1-12, 2020.2235-2988https://www.arca.fiocruz.br/handle/icict/4491810.3389/fcimb.2020.591121engFrontiers MediaImmunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Goiânia, GO, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Histoplasmosis is one of the most frequent systemic mycosis in HIV patients. In these patients, histoplasmosis has high rates of morbidity/mortality if diagnosis and treatment are delayed. Despite its relevance, there is a paucity of information concerning the interaction between Histoplasma capsulatum and the human host, especially regarding the B-cell response, which has a direct impact on the diagnosis. Culture-based "gold-standard" methods have limitations, making immunodiagnostic tests an attractive option for clinical decisions. Despite the continuous development of those tests, improving serological parameters is necessary to make these methods efficient tools for definitive diagnosis of histoplasmosis. This includes the determination of more specific and immunogenic antigens to improve specificity and sensitivity of assays. In this study, we performed a co-immunoprecipitation assay between a protein extract from the yeast form of H. capsulatum and pooled sera from patients with proven histoplasmosis, followed by shotgun mass spectrometry identification of antigenic targets. Sera from patients with other pulmonary infections or from healthy individuals living in endemic areas of histoplasmosis were also assayed to determine potentially cross-reactive proteins. The primary structures of H. capsulatum immunoprecipitated proteins were evaluated using the DNAStar Protean 7.0 software. In parallel, the online epitope prediction server, BCPREDS, was used to complement the B-epitope prediction analysis. Our approach detected 132 reactive proteins to antibodies present in histoplasmosis patients' sera. Among these antigens, 127 were recognized also by antibodies in heterologous patients' and/or normal healthy donors' sera. Therefore, the only three antigens specifically recognized by antibodies of histoplasmosis patients were mapped as potential antigenic targets: the M antigen, previously demonstrated in the diagnosis of histoplasmosis, and the catalase P and YPS-3 proteins, characterized as virulence factors of H. capsulatum, with antigenic properties still unclear. The other two proteins were fragments of the YPS-3 and M antigen. Overlapping results obtained from the two aforementioned bioinformatic tools, 16 regions from these three proteins are proposed as putative B-cell epitopes exclusive to H. capsulatum. These data reveal a new role for these proteins on H. capsulatum interactions with the immune system and indicate their possible use in new methods for the diagnosis of histoplasmosis.Histoplasma capsulatumHomo sapiensM antigenYPS-3Catalase PEpitopesImmunoproteomeMass spectrometryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/44918/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALImmunoproteomics_Marcos_Almeida_etal_INI_2020.pdfImmunoproteomics_Marcos_Almeida_etal_INI_2020.pdfapplication/pdf1794439https://www.arca.fiocruz.br/bitstream/icict/44918/2/Immunoproteomics_Marcos_Almeida_etal_INI_2020.pdf28b232c76941c0c94169fe8aa81c7b04MD52TEXTImmunoproteomics_Marcos_Almeida_etal_INI_2020.pdf.txtImmunoproteomics_Marcos_Almeida_etal_INI_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
title Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
spellingShingle Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
Almeida, Marcos Abreu
Histoplasma capsulatum
Homo sapiens
M antigen
YPS-3
Catalase P
Epitopes
Immunoproteome
Mass spectrometry
title_short Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
title_full Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
title_fullStr Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
title_full_unstemmed Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
title_sort Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
author Almeida, Marcos Abreu
author_facet Almeida, Marcos Abreu
Almeida-Paes, Rodrigo
Guimarães, Allan Jefferson
Valente, Richard Hemmi
Soares, Célia Maria de Almeida
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Almeida-Paes, Rodrigo
Guimarães, Allan Jefferson
Valente, Richard Hemmi
Soares, Célia Maria de Almeida
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Marcos Abreu
Almeida-Paes, Rodrigo
Guimarães, Allan Jefferson
Valente, Richard Hemmi
Soares, Célia Maria de Almeida
Zancopé-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv Histoplasma capsulatum
Homo sapiens
M antigen
YPS-3
Catalase P
Epitopes
Immunoproteome
Mass spectrometry
topic Histoplasma capsulatum
Homo sapiens
M antigen
YPS-3
Catalase P
Epitopes
Immunoproteome
Mass spectrometry
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-12-15T19:26:34Z
dc.date.available.fl_str_mv 2020-12-15T19:26:34Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Marcos Abreu et al. Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens-Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis. Frontiers in Cellular and Infection Microbiology, v. 10, p. 1-12, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44918
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2020.591121
identifier_str_mv ALMEIDA, Marcos Abreu et al. Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens-Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis. Frontiers in Cellular and Infection Microbiology, v. 10, p. 1-12, 2020.
2235-2988
10.3389/fcimb.2020.591121
url https://www.arca.fiocruz.br/handle/icict/44918
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44918/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/44918/2/Immunoproteomics_Marcos_Almeida_etal_INI_2020.pdf
https://www.arca.fiocruz.br/bitstream/icict/44918/3/Immunoproteomics_Marcos_Almeida_etal_INI_2020.pdf.txt
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