Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil

Detalhes bibliográficos
Autor(a) principal: De Carli, Silvia
Data de Publicação: 2020
Outros Autores: Wolf, Jonas Michel, Gräf, Tiago, Lehmann, Fernanda K. M., Fonseca, André S. K., Canal, Cláudio W., Lungea, Vagner R., Ikuta, Nilo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44046
Resumo: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [311010/ 2017-2], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [Finance Code 001], Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and Pro Reitoria de Pesquisa Universidade Federal do Rio Grande do Sul.
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spelling De Carli, SilviaWolf, Jonas MichelGräf, TiagoLehmann, Fernanda K. M.Fonseca, André S. K.Canal, Cláudio W.Lungea, Vagner R.Ikuta, Nilo2020-10-19T12:26:53Z2020-10-19T12:26:53Z2020DE CARLI, Silvia et al. Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil. Avian Pathology, 2020.0307-9457https://www.arca.fiocruz.br/handle/icict/4404610.1080/03079457.2020.1804528engTaylor & FrancisARVTenossinoviteArtriteFilogeniaEvolução viralARVTenosynovitisArthritisMalabsorption syndromePhylogenyViral evolutionGenotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [311010/ 2017-2], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [Finance Code 001], Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and Pro Reitoria de Pesquisa Universidade Federal do Rio Grande do Sul.Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil / Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre, RS, Brasil.Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil.Simbios Biotecnologia. Cachoeirinha, RS, Brasil.Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre, RS, Brasil.Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil / Simbios Biotecnologia. Cachoeirinha, RS, Brasil.Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil / Simbios Biotecnologia. Cachoeirinha, RS, Brasil.Avian reovirus (ARV) is one of the main causes of infectious arthritis/tenosynovitis and malabsorption syndrome (MAS) in poultry. ARVs have been disseminated in Brazilian poultry flocks in the last years. This study aimed to genotype ARVs and to evaluate the molecular evolution of the more frequent ARV lineages detected in Brazilian poultry-producing farms. A total of 100 poultry flocks with clinical signs of tenosynovitis/MAS, from all Brazilian poultryproducing regions were positive for ARV by PCR. Seventeen bird tissues were submitted to cell culture and ARV RNA detection/genotyping by two PCRs. The phylogenetic classification was based on σC gene alignment using a dataset with other Brazilian and worldwide ARVs sequences. ARVs were specifically detected by both PCRs from the 17 cell cultures, and σC gene partial fragments were sequenced. All these sequences were aligned with a total of 451 ARV σC gene data available in GenBank. Phylogenetic analysis demonstrated five welldefined clusters that were classified into lineages I, II, III, IV, and V. Three lineages could be further divided into sub-lineages: I (I vaccine, Ia, Ib), II (IIa, IIb, IIc) and IV (IVa and IVb). Brazilian ARVs were from four lineages/sub-lineages: Ib (48.2%), IIb (22.2%), III (3.7%) and V (25.9%). The Bayesian analysis demonstrated that the most frequent sub-lineage Ib emerged in the world around 1968 and it was introduced into Brazil in 2010, with increasing spread soon after. In conclusion, four different ARV lineages are circulating in Brazilian poultry flocks, all associated with clinical diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44046/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarli, S. Genotypic Characterization....pdfCarli, S. Genotypic Characterization....pdfapplication/pdf2333172https://www.arca.fiocruz.br/bitstream/icict/44046/2/Carli%2c%20S.%20Genotypic%20Characterization....pdf02275a046678f9380fc55919cdce8482MD52TEXTCarli, S. Genotypic Characterization....pdf.txtCarli, S. 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dc.title.pt_BR.fl_str_mv Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
title Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
spellingShingle Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
De Carli, Silvia
ARV
Tenossinovite
Artrite
Filogenia
Evolução viral
ARV
Tenosynovitis
Arthritis
Malabsorption syndrome
Phylogeny
Viral evolution
title_short Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
title_full Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
title_fullStr Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
title_full_unstemmed Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
title_sort Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil
author De Carli, Silvia
author_facet De Carli, Silvia
Wolf, Jonas Michel
Gräf, Tiago
Lehmann, Fernanda K. M.
Fonseca, André S. K.
Canal, Cláudio W.
Lungea, Vagner R.
Ikuta, Nilo
author_role author
author2 Wolf, Jonas Michel
Gräf, Tiago
Lehmann, Fernanda K. M.
Fonseca, André S. K.
Canal, Cláudio W.
Lungea, Vagner R.
Ikuta, Nilo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv De Carli, Silvia
Wolf, Jonas Michel
Gräf, Tiago
Lehmann, Fernanda K. M.
Fonseca, André S. K.
Canal, Cláudio W.
Lungea, Vagner R.
Ikuta, Nilo
dc.subject.other.pt_BR.fl_str_mv ARV
Tenossinovite
Artrite
Filogenia
Evolução viral
topic ARV
Tenossinovite
Artrite
Filogenia
Evolução viral
ARV
Tenosynovitis
Arthritis
Malabsorption syndrome
Phylogeny
Viral evolution
dc.subject.en.pt_BR.fl_str_mv ARV
Tenosynovitis
Arthritis
Malabsorption syndrome
Phylogeny
Viral evolution
description Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [311010/ 2017-2], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [Finance Code 001], Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and Pro Reitoria de Pesquisa Universidade Federal do Rio Grande do Sul.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-19T12:26:53Z
dc.date.available.fl_str_mv 2020-10-19T12:26:53Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DE CARLI, Silvia et al. Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil. Avian Pathology, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44046
dc.identifier.issn.pt_BR.fl_str_mv 0307-9457
dc.identifier.doi.none.fl_str_mv 10.1080/03079457.2020.1804528
identifier_str_mv DE CARLI, Silvia et al. Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil. Avian Pathology, 2020.
0307-9457
10.1080/03079457.2020.1804528
url https://www.arca.fiocruz.br/handle/icict/44046
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44046/1/license.txt
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