Effect of gedunin on acute articular inflammation and hypernociception in mice

Bibliographic Details
Main Author: Conte, Fernando P.
Publication Date: 2015
Other Authors: Ferraris, Fausto K., Costa, Thadeu E. M. M., Pacheco, Patricia, Seito, Leonardo N., Verri, Waldiceu A., Cunha, Fernando Q., Penido, Carmen, Henriques, Maria G.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/59630
Summary: Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil.
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spelling Conte, Fernando P.Ferraris, Fausto K.Costa, Thadeu E. M. M.Pacheco, PatriciaSeito, Leonardo N.Verri, Waldiceu A.Cunha, Fernando Q.Penido, CarmenHenriques, Maria G.2023-07-18T04:01:52Z2023-07-18T04:01:52Z2015CONTE, Fernando P. et al. Effect of gedunin on acute articular inflammation and hypernociception in mice. Molecules, v. 20, n. 2, p. 2636–2657, Feb. 2015.1420-3049https://www.arca.fiocruz.br/handle/icict/5963010.3390/MOLECULES200226361420-3049engMDPIAnimalsAnti-Inflammatory Agents, Non-SteroidalCartilage, ArticularCell SurvivalEndothelin-1Inflammation MediatorsKnee JointLimoninsMacrophage ActivationMaleMice, Inbred C57BLNeutrophil InfiltrationNociceptionOsteochondritisZymosanArthritisEdemaNeutrophilMacrophageLipid bodyCytokineLeukotrieneProstaglandinEffect of gedunin on acute articular inflammation and hypernociception in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Londrina, PR, Brazil / Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil.Gedunin, a natural limonoid from Meliaceae species, has been previously described as an antiinflammatory compound in experimental models of allergic inflammation. Here, we report the antiinflammatory and antinociceptive effects of gedunin in an acute model of articular inflammation induced by zymosan (500 μg/cavity; intra-articular) in C57BL/6 mice. Intraperitoneal (i.p.) pretreatment with gedunin (0.005-5 mg/kg) impaired zymosan-induced edema formation, neutrophil accumulation and hypernociception in mouse knee joints, due to decreased expression of preproET-1 mRNA and production of LTB4, PGE2, TNF-α and IL-6. Mouse post-treatment with gedunin (0.05 mg/kg; i.p.) 1 and 6 h after stimulation also impaired articular inflammation, by reverting edema formation, neutrophil accumulation and the production of lipid mediators, cytokines and endothelin. In addition, gedunin directly modulated the functions of neutrophils and macrophages in vitro. The pre-incubation of neutrophil with gedunin (100 µM) impaired shape change, adhesion to endothelial cells, chemotaxis and lipid body formation triggered by different stimuli. Macrophage pretreatment with gedunin impaired intracellular calcium mobilization, nitric oxide production, inducible nitric oxide synthase expression and induced the expression of the antiinflammatory chaperone heat shock protein 70. Our results demonstrate that gedunin presents remarkable antiinflammatory and anti-nociceptive effects on zymosan-induced inflamed knee joints, modulating different cell populations.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59630/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL10.3390_molecules20022636.pdfapplication/pdf2391542https://www.arca.fiocruz.br/bitstream/icict/59630/2/10.3390_molecules20022636.pdf897bd199f56e7bb0274741652637dfafMD52icict/596302023-07-18 22:00:41.044oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-19T01:00:41Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Effect of gedunin on acute articular inflammation and hypernociception in mice
title Effect of gedunin on acute articular inflammation and hypernociception in mice
spellingShingle Effect of gedunin on acute articular inflammation and hypernociception in mice
Conte, Fernando P.
Animals
Anti-Inflammatory Agents, Non-Steroidal
Cartilage, Articular
Cell Survival
Endothelin-1
Inflammation Mediators
Knee Joint
Limonins
Macrophage Activation
Male
Mice, Inbred C57BL
Neutrophil Infiltration
Nociception
Osteochondritis
Zymosan
Arthritis
Edema
Neutrophil
Macrophage
Lipid body
Cytokine
Leukotriene
Prostaglandin
title_short Effect of gedunin on acute articular inflammation and hypernociception in mice
title_full Effect of gedunin on acute articular inflammation and hypernociception in mice
title_fullStr Effect of gedunin on acute articular inflammation and hypernociception in mice
title_full_unstemmed Effect of gedunin on acute articular inflammation and hypernociception in mice
title_sort Effect of gedunin on acute articular inflammation and hypernociception in mice
author Conte, Fernando P.
author_facet Conte, Fernando P.
Ferraris, Fausto K.
Costa, Thadeu E. M. M.
Pacheco, Patricia
Seito, Leonardo N.
Verri, Waldiceu A.
Cunha, Fernando Q.
Penido, Carmen
Henriques, Maria G.
author_role author
author2 Ferraris, Fausto K.
Costa, Thadeu E. M. M.
Pacheco, Patricia
Seito, Leonardo N.
Verri, Waldiceu A.
Cunha, Fernando Q.
Penido, Carmen
Henriques, Maria G.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Conte, Fernando P.
Ferraris, Fausto K.
Costa, Thadeu E. M. M.
Pacheco, Patricia
Seito, Leonardo N.
Verri, Waldiceu A.
Cunha, Fernando Q.
Penido, Carmen
Henriques, Maria G.
dc.subject.mesh.en_US.fl_str_mv Animals
Anti-Inflammatory Agents, Non-Steroidal
Cartilage, Articular
Cell Survival
Endothelin-1
Inflammation Mediators
Knee Joint
Limonins
Macrophage Activation
Male
Mice, Inbred C57BL
Neutrophil Infiltration
Nociception
Osteochondritis
topic Animals
Anti-Inflammatory Agents, Non-Steroidal
Cartilage, Articular
Cell Survival
Endothelin-1
Inflammation Mediators
Knee Joint
Limonins
Macrophage Activation
Male
Mice, Inbred C57BL
Neutrophil Infiltration
Nociception
Osteochondritis
Zymosan
Arthritis
Edema
Neutrophil
Macrophage
Lipid body
Cytokine
Leukotriene
Prostaglandin
dc.subject.en.en_US.fl_str_mv Zymosan
Arthritis
Edema
Neutrophil
Macrophage
Lipid body
Cytokine
Leukotriene
dc.subject.en.none.fl_str_mv Prostaglandin
description Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos - Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brazil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2023-07-18T04:01:52Z
dc.date.available.fl_str_mv 2023-07-18T04:01:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CONTE, Fernando P. et al. Effect of gedunin on acute articular inflammation and hypernociception in mice. Molecules, v. 20, n. 2, p. 2636–2657, Feb. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59630
dc.identifier.issn.en_US.fl_str_mv 1420-3049
dc.identifier.doi.none.fl_str_mv 10.3390/MOLECULES20022636
dc.identifier.eissn.none.fl_str_mv 1420-3049
identifier_str_mv CONTE, Fernando P. et al. Effect of gedunin on acute articular inflammation and hypernociception in mice. Molecules, v. 20, n. 2, p. 2636–2657, Feb. 2015.
1420-3049
10.3390/MOLECULES20022636
url https://www.arca.fiocruz.br/handle/icict/59630
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59630/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/59630/2/10.3390_molecules20022636.pdf
bitstream.checksum.fl_str_mv 8a4605be74aa9ea9d79846c1fba20a33
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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