Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Degrave, Wim
Data de Publicação: 1988
Outros Autores: Fragoso, Stenio P., Britto, Constança, van Heuverswyn, Hugo, Kidane, Getachew Z., Cardoso, Maria A. B., Mueller, Rita U., Simpson, Larry, Morel, Carlos Medicis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14598
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Degrave, WimFragoso, Stenio P.Britto, Constançavan Heuverswyn, HugoKidane, Getachew Z.Cardoso, Maria A. B.Mueller, Rita U.Simpson, LarryMorel, Carlos Medicis2016-06-29T12:39:45Z2016-07-07T11:40:02Z2016-06-29T12:39:45Z2016-07-07T11:40:02Z1988DEGRAVE, Wim; et al. Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi. Molecular and Biochemical Parasitology, v.27, p.63-70 , 1988.0166-6851https://www.arca.fiocruz.br/handle/icict/1459810.1016/0166-6851(88)90025-41872-9428engElsevierPeculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.University of California. Biology and Molecular Biology Institute. Los Alngeles, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.The sequences of two minicircles from the kinetoplast DNA of the CL strain and one of the Y strain of Trypanosoma cruzi are reported. These 1.4 kb molecules have a peculiar sequence organization, the most distinctive feature being the occurrence of a 120 bp sequence repeated four times, located at 0, 90, 180 and 270 degrees along each circle. We have termed these conserved regions in this species 'minirepeats'. Minirepeats have a 3-fold higher concentration of cytosine residues in comparison with the variable regions and contain the universal 12-mer motif GGGGTTGGTGTA present in all sequenced minicircles and which was shown to be involved in DNA replication. A consensus sequence of T. cruzi minirepeats was determined using the 20 minirepeats present in five known T. cruzi minicircle sequences. This consensus sequence contains regions which have been remarkably well preserved in strains which show great biological diversity. In addition a low level of intraminicircle sequence similarity was also observed within the variable region, but this similarity did not extend between strains. The abundance of conserved minirepeat sequences containing invariant restriction sites in T. cruzi cells may prove valuable for the development of new direct diagnostic methods for Chagas' disease based on DNA probe technology.Trypanosoma cruziKinetoplast DNA sequenceMinicircle DNADNA sequenceTrypanosoma cruziSequência de BasesDNA de Cinetoplastoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTcarlos9_morel_etal_IOC_1988.pdf.txtcarlos9_morel_etal_IOC_1988.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi
title Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi
spellingShingle Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi
Degrave, Wim
Trypanosoma cruzi
Kinetoplast DNA sequence
Minicircle DNA
DNA sequence
Trypanosoma cruzi
Sequência de Bases
DNA de Cinetoplasto
title_short Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi
title_full Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi
title_fullStr Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi
title_full_unstemmed Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi
title_sort Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi
author Degrave, Wim
author_facet Degrave, Wim
Fragoso, Stenio P.
Britto, Constança
van Heuverswyn, Hugo
Kidane, Getachew Z.
Cardoso, Maria A. B.
Mueller, Rita U.
Simpson, Larry
Morel, Carlos Medicis
author_role author
author2 Fragoso, Stenio P.
Britto, Constança
van Heuverswyn, Hugo
Kidane, Getachew Z.
Cardoso, Maria A. B.
Mueller, Rita U.
Simpson, Larry
Morel, Carlos Medicis
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Degrave, Wim
Fragoso, Stenio P.
Britto, Constança
van Heuverswyn, Hugo
Kidane, Getachew Z.
Cardoso, Maria A. B.
Mueller, Rita U.
Simpson, Larry
Morel, Carlos Medicis
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Kinetoplast DNA sequence
Minicircle DNA
DNA sequence
topic Trypanosoma cruzi
Kinetoplast DNA sequence
Minicircle DNA
DNA sequence
Trypanosoma cruzi
Sequência de Bases
DNA de Cinetoplasto
dc.subject.decs.pt_BR.fl_str_mv Trypanosoma cruzi
Sequência de Bases
DNA de Cinetoplasto
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 1988
dc.date.issued.fl_str_mv 1988
dc.date.accessioned.fl_str_mv 2016-06-29T12:39:45Z
2016-07-07T11:40:02Z
dc.date.available.fl_str_mv 2016-06-29T12:39:45Z
2016-07-07T11:40:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv DEGRAVE, Wim; et al. Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi. Molecular and Biochemical Parasitology, v.27, p.63-70 , 1988.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14598
dc.identifier.issn.pt_BR.fl_str_mv 0166-6851
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/0166-6851(88)90025-4
dc.identifier.eissn.pt_BR.fl_str_mv 1872-9428
identifier_str_mv DEGRAVE, Wim; et al. Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi. Molecular and Biochemical Parasitology, v.27, p.63-70 , 1988.
0166-6851
10.1016/0166-6851(88)90025-4
1872-9428
url https://www.arca.fiocruz.br/handle/icict/14598
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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