Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23468 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil. |
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Silva Jr., J. V. J.Arenhart, S.Santos, H. F.Almeida-Queiroz, S. R.Silva, A. N. M. R.Trevisol, I. M.Bertani, G. R.Gil, L. H. V. G.2017-11-30T13:15:29Z2017-11-30T13:15:29Z2014SILVA JR., J. V. J. et al. Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast. Brazilian Journal of Microbiology, v. 45, n. 4, p. 1555–1563, dez. 2014.1678-4405https://www.arca.fiocruz.br/handle/icict/2346810.1590/S1517-83822014000400054engVírus da Doença Infecciosa da BursaGenética Reversarecombinação homóloga baseada em fermentoinfectious bursal disease virusreverse geneticsyeast-based homologous recombinationAnimaisEmbrião de GalinhaRecombinação HomólogaVetores GenéticosVírus da Doença Infecciosa da BursagenéticaGenética ReversamétodosBrasilCélulas CultivadasFibroblastosvirologiaInstabilidade GenômicaTransfecçãoVírus da Doença Infecciosa da Bursaisolamento & purificaçãoVírus da Doença Infecciosa da BursafisiologiaSaccharomyces cerevisiaegenéticaCultura de VírusReplicação ViralEfficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeastinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / Universidade Federal de Santa Maria. Centro de Ciências Rurais. Setor de Virologia. Departamento de Medicina Veterinária Preventiva. Santa Maria, RS, Brazil.Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Virologia. Porto Alegre, RS, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Embrapa Suínos e Aves . Concórdia, SC, Brazil.Universidade Federal de Pernambuco. Departamento de Bioquímica e Laboratório de Imunopatologia Keiso Asami. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The aim of this research was to build the world's first VRG for IBDV by yeast-based homologous recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br) was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The clones were transfected into chicken embryo fibroblasts and the recovered virus (IC-IBDV-Br) showed genetic stability and similar phenotype to Wt-IBDV-Br, which were observed by nucleotide sequence, focus size/morphology and replication kinetics, respectively. Thus, IBDV reverse genetics by yeast-based homologous recombination provides tools to IBDV understanding and vaccines/viral vectors development.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23468/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. Efficient assembly - silva junior.pdfart. Efficient assembly - silva junior.pdfapplication/pdf747517https://www.arca.fiocruz.br/bitstream/icict/23468/2/art.%20Efficient%20assembly%20-%20silva%20junior.pdf26680757bde3c6d15c519a2aef1ec8b0MD52TEXTart. Efficient assembly - silva junior.pdf.txtart. 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| dc.title.pt_BR.fl_str_mv |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast |
| title |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast |
| spellingShingle |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast Silva Jr., J. V. J. Vírus da Doença Infecciosa da Bursa Genética Reversa recombinação homóloga baseada em fermento infectious bursal disease virus reverse genetics yeast-based homologous recombination Animais Embrião de Galinha Recombinação Homóloga Vetores Genéticos Vírus da Doença Infecciosa da Bursa genética Genética Reversa métodos Brasil Células Cultivadas Fibroblastos virologia Instabilidade Genômica Transfecção Vírus da Doença Infecciosa da Bursa isolamento & purificação Vírus da Doença Infecciosa da Bursa fisiologia Saccharomyces cerevisiae genética Cultura de Vírus Replicação Viral |
| title_short |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast |
| title_full |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast |
| title_fullStr |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast |
| title_full_unstemmed |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast |
| title_sort |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast |
| author |
Silva Jr., J. V. J. |
| author_facet |
Silva Jr., J. V. J. Arenhart, S. Santos, H. F. Almeida-Queiroz, S. R. Silva, A. N. M. R. Trevisol, I. M. Bertani, G. R. Gil, L. H. V. G. |
| author_role |
author |
| author2 |
Arenhart, S. Santos, H. F. Almeida-Queiroz, S. R. Silva, A. N. M. R. Trevisol, I. M. Bertani, G. R. Gil, L. H. V. G. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva Jr., J. V. J. Arenhart, S. Santos, H. F. Almeida-Queiroz, S. R. Silva, A. N. M. R. Trevisol, I. M. Bertani, G. R. Gil, L. H. V. G. |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da Doença Infecciosa da Bursa Genética Reversa recombinação homóloga baseada em fermento |
| topic |
Vírus da Doença Infecciosa da Bursa Genética Reversa recombinação homóloga baseada em fermento infectious bursal disease virus reverse genetics yeast-based homologous recombination Animais Embrião de Galinha Recombinação Homóloga Vetores Genéticos Vírus da Doença Infecciosa da Bursa genética Genética Reversa métodos Brasil Células Cultivadas Fibroblastos virologia Instabilidade Genômica Transfecção Vírus da Doença Infecciosa da Bursa isolamento & purificação Vírus da Doença Infecciosa da Bursa fisiologia Saccharomyces cerevisiae genética Cultura de Vírus Replicação Viral |
| dc.subject.en.pt_BR.fl_str_mv |
infectious bursal disease virus reverse genetics yeast-based homologous recombination |
| dc.subject.decs.pt_BR.fl_str_mv |
Animais Embrião de Galinha Recombinação Homóloga Vetores Genéticos Vírus da Doença Infecciosa da Bursa genética Genética Reversa métodos Brasil Células Cultivadas Fibroblastos virologia Instabilidade Genômica Transfecção Vírus da Doença Infecciosa da Bursa isolamento & purificação Vírus da Doença Infecciosa da Bursa fisiologia Saccharomyces cerevisiae genética Cultura de Vírus Replicação Viral |
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Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil. |
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2014 |
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2014 |
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2017-11-30T13:15:29Z |
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2017-11-30T13:15:29Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SILVA JR., J. V. J. et al. Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast. Brazilian Journal of Microbiology, v. 45, n. 4, p. 1555–1563, dez. 2014. |
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https://www.arca.fiocruz.br/handle/icict/23468 |
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1678-4405 |
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10.1590/S1517-83822014000400054 |
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SILVA JR., J. V. J. et al. Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast. Brazilian Journal of Microbiology, v. 45, n. 4, p. 1555–1563, dez. 2014. 1678-4405 10.1590/S1517-83822014000400054 |
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