Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/39312 |
Resumo: | Fundação de Amparo à Pesquisa do Estado da Bahia (Fapesb. http://www.fapes b.ba.gov.br) Grant No. APR0434/2005 |
| id |
CRUZ_9a0a1e59af956b5d24d07e12c231aa1e |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/39312 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Lima, Isadora dos SantosSolcá, Manuela da SilvaTafuri, Wagner LuizFreitas, Luiz Antonio Rodrigues deSantos, Washington Luis Conrado dos2020-01-20T12:27:29Z2020-01-20T12:27:29Z2019LIMA, Isadora dos Santos et al. Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum. Parasites and Vectors, v. 12, n. 487, p. 1-14, 16 Oct. 2019.1756-3305https://www.arca.fiocruz.br/handle/icict/3931210.1186/s13071-019-3723-1Fundação de Amparo à Pesquisa do Estado da Bahia (Fapesb. http://www.fapes b.ba.gov.br) Grant No. APR0434/2005Grant No. DTE0037/2011Oswaldo Cruz Foundation (Fiocruz; http://portal.fiocruz.br/pt-br) PAPES V, grant 403470/2008-0Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Background: The liver plays a central role in the development of canine visceral leishmaniasis. Studies of natural infection in animals and humans indicate a direct relationship between resolution of infection and the formation and maturation of granulomas in the liver. However, in contrast to other reports in the literature, the present study found no diferences in the characteristics of hepatic granulomas that could be related to resistance or susceptibility to Leishmania. Here, we describe the hepatic alterations observed in dogs with difering clinical manifestations of visceral leishmaniasis in an endemic area in the state of Bahia, Brazil. Methods: We examined 148 animals in an endemic area. The animals were clinically examined, and the infection was determined by ELISA, spleen aspirate culture and quantitative PCR. The animals were grouped into asymptomatic or symptomatic based on the number of signs of LV. The histological liver evaluation was performed in a blinded way. Results: Our results indicated no association between the characteristics of granulomas and clinical presentation. We found an association between the intensity of this infammatory response and parasite load in the animals’ spleens. It is important to note that while hepatic alterations, such as portal and perivascular infammation and the presence of larger amounts of granulomas, were linked with higher parasite loads, we found the inverse to be true with respect to intrasinusoidal lymphocytosis, the formation of intrasinusoidal infammatory cell aggregates and Kupfer cell hypertrophy. Conclusions: Our fndings suggest that the presence of mononuclear infammatory cells inside the sinusoids is more important than that of organized granulomas in terms of the containment of parasitism by the host. We suggest that the presence of granulomas indicates the failure of a frst line of defense mechanism in the control of parasite infection, which could be related to the presence of infammatory cells and Kupfer cell hypertrophy inside the sinusoids. We further demonstrated that dogs with active Leishmania spp. infection present a higher frequency of infammatory changes in the liver. In addition to being correlated with the severity of clinical manifestation, these hepatic alterations were also associated with changes in hematological and biochemical parameters.engBMCLeishmania infantumLeishmaniose visceral caninaHistopatologia hepáticaPatologiaInflamaçãoGranulomaLeishmania infantumCanine visceral leishmaniasisLiver histopathologyPathologyInfammationGranulomaAssessment of histological liver alterations in dogs naturally infected with Leishmania infantuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39312/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLima I S Assessment of ...Parasites & Vectors.pdfLima I S Assessment of ...Parasites & Vectors.pdfapplication/pdf2867832https://www.arca.fiocruz.br/bitstream/icict/39312/2/Lima%20I%20S%20Assessment%20of%20...Parasites%20%26%20Vectors.pdf4f6943af6c3a941b6399108be47ed653MD52TEXTLima I S Assessment of ...Parasites & Vectors.pdf.txtLima I S Assessment of ...Parasites & Vectors.pdf.txtExtracted texttext/plain52748https://www.arca.fiocruz.br/bitstream/icict/39312/3/Lima%20I%20S%20Assessment%20of%20...Parasites%20%26%20Vectors.pdf.txt4f21d3c17f278dfbbf2d4f61b2c971b0MD53icict/393122023-03-15 14:33:28.829oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:28Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en.fl_str_mv |
Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum |
| title |
Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum |
| spellingShingle |
Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum Lima, Isadora dos Santos Leishmania infantum Leishmaniose visceral canina Histopatologia hepática Patologia Inflamação Granuloma Leishmania infantum Canine visceral leishmaniasis Liver histopathology Pathology Infammation Granuloma |
| title_short |
Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum |
| title_full |
Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum |
| title_fullStr |
Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum |
| title_full_unstemmed |
Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum |
| title_sort |
Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum |
| author |
Lima, Isadora dos Santos |
| author_facet |
Lima, Isadora dos Santos Solcá, Manuela da Silva Tafuri, Wagner Luiz Freitas, Luiz Antonio Rodrigues de Santos, Washington Luis Conrado dos |
| author_role |
author |
| author2 |
Solcá, Manuela da Silva Tafuri, Wagner Luiz Freitas, Luiz Antonio Rodrigues de Santos, Washington Luis Conrado dos |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Isadora dos Santos Solcá, Manuela da Silva Tafuri, Wagner Luiz Freitas, Luiz Antonio Rodrigues de Santos, Washington Luis Conrado dos |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania infantum Leishmaniose visceral canina Histopatologia hepática Patologia Inflamação Granuloma |
| topic |
Leishmania infantum Leishmaniose visceral canina Histopatologia hepática Patologia Inflamação Granuloma Leishmania infantum Canine visceral leishmaniasis Liver histopathology Pathology Infammation Granuloma |
| dc.subject.en.en.fl_str_mv |
Leishmania infantum Canine visceral leishmaniasis Liver histopathology Pathology Infammation Granuloma |
| description |
Fundação de Amparo à Pesquisa do Estado da Bahia (Fapesb. http://www.fapes b.ba.gov.br) Grant No. APR0434/2005 |
| publishDate |
2019 |
| dc.date.issued.fl_str_mv |
2019 |
| dc.date.accessioned.fl_str_mv |
2020-01-20T12:27:29Z |
| dc.date.available.fl_str_mv |
2020-01-20T12:27:29Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
LIMA, Isadora dos Santos et al. Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum. Parasites and Vectors, v. 12, n. 487, p. 1-14, 16 Oct. 2019. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/39312 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1756-3305 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/s13071-019-3723-1 |
| identifier_str_mv |
LIMA, Isadora dos Santos et al. Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum. Parasites and Vectors, v. 12, n. 487, p. 1-14, 16 Oct. 2019. 1756-3305 10.1186/s13071-019-3723-1 |
| url |
https://www.arca.fiocruz.br/handle/icict/39312 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
BMC |
| publisher.none.fl_str_mv |
BMC |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/39312/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/39312/2/Lima%20I%20S%20Assessment%20of%20...Parasites%20%26%20Vectors.pdf https://www.arca.fiocruz.br/bitstream/icict/39312/3/Lima%20I%20S%20Assessment%20of%20...Parasites%20%26%20Vectors.pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 4f6943af6c3a941b6399108be47ed653 4f21d3c17f278dfbbf2d4f61b2c971b0 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1798325066354655232 |