Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection

Detalhes bibliográficos
Autor(a) principal: Losada-Barragán, Monica
Data de Publicação: 2019
Outros Autores: Umaña-Pérez, Adriana, Durães, Jonathan, Cuervo-Escobar, Sergio, Rodríguez-Vega, Andrés, Ribeiro-Gomes, Flávia L., Berbert, Luiz R., Morgado, Fernanda, Porrozzi, Renato, Cruz, Daniella Arêas Mendes da, Aquino, Priscila, Carvalho, Paulo C., Savino, Wilson, Sánchez-Gómez, Myriam, Padrón, Gabriel, Cuervo, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35518
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil / Universidad Antonio Nariño. Departamento de Biologia. Grupo de Investigación en Biología Celular y Funcional e Ingeniería de Biomoléculas. Bogotá, Colombia.
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spelling Losada-Barragán, MonicaUmaña-Pérez, AdrianaDurães, JonathanCuervo-Escobar, SergioRodríguez-Vega, AndrésRibeiro-Gomes, Flávia L.Berbert, Luiz R.Morgado, FernandaPorrozzi, RenatoCruz, Daniella Arêas Mendes daAquino, PriscilaCarvalho, Paulo C.Savino, WilsonSánchez-Gómez, MyriamPadrón, GabrielCuervo, Patricia2019-09-12T12:55:09Z2019-09-12T12:55:09Z2019LOSADA-BARRAGÁN, Monica et al. Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-19, July 2019.2235-2988https://www.arca.fiocruz.br/handle/icict/3551810.3389/fcimb.2019.00252engFrontiers MediaLeishmania infantumTimoMicroambiente tímicoDesnutrição protéicaFluido intersticialLeishmaniose visceralProteômicaOxidação de ácidos graxosThymusThymic microenvironmentProtein malnutritionLeishmania infantumVisceral leishmaniasisInterstitial fluidProteomicsFatty acid oxidationThymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil / Universidad Antonio Nariño. Departamento de Biologia. Grupo de Investigación en Biología Celular y Funcional e Ingeniería de Biomoléculas. Bogotá, Colombia.Universidad Nacional de Colombia. Departamento de Química. Grupo de Investigación en Hormonas. Bogotá, Colombia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.Universidad de Ciencias Aplicadas y Ambientales. Facultad de Ciencias. Bogotá, Colombia;Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Grupo de Espectrometria de Massa Computacional e Proteômica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Universidad Nacional de Colombia. Departamento de Química. Grupo de Investigación en Hormonas. Bogotá, Colombia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.Detrimental effects of malnutrition on immune responses to pathogens have long been recognized and it is considered a main risk factor for various infectious diseases, including visceral leishmaniasis (VL). Thymus is a target of both malnutrition and infection, but its role in the immune response to Leishmania infantum in malnourished individuals is barely studied. Because we previously observed thymic atrophy and significant reduction in cellularity and chemokine levels in malnourished mice infected with L. infantum, we postulated that the thymic microenvironment is severely compromised in those animals. To test this, we analyzed the microarchitecture of the organ and measured the protein abundance in its interstitial space in malnourished BALB/c mice infected or not with L. infantum. Malnourished-infected animals exhibited a significant reduction of the thymic cortex:medulla ratio and altered abundance of proteins secreted in the thymic interstitial fluid. Eighty-one percent of identified proteins are secreted by exosomes and malnourished-infected mice showed significant decrease in exosomal proteins, suggesting that exosomal carrier system, and therefore intrathymic communication, is dysregulated in those animals. Malnourished-infected mice also exhibited a significant increase in the abundance of proteins involved in lipid metabolism and tricarboxylic acid cycle, suggestive of a non-proliferative microenvironment. Accordingly, flow cytometry analysis revealed decreased proliferation of single positive and double positive T cells in those animals. Together, the reduced cortical area, decreased proliferation, and altered protein abundance suggest a dysfunctional thymic microenvironment where T cell migration, proliferation, and maturation are compromised, contributing for the thymic atrophy observed in malnourished animals. All these alterations could affect the control of the local and systemic infection, resulting in an impaired response to L. infantum infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35518/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALWilsonSavino_PatriciaCuervo_etal_IOC_2019.pdfWilsonSavino_PatriciaCuervo_etal_IOC_2019.pdfapplication/pdf1666003https://www.arca.fiocruz.br/bitstream/icict/35518/2/WilsonSavino_PatriciaCuervo_etal_IOC_2019.pdff8b318e17c7664980b5450209f86d956MD52TEXTWilsonSavino_PatriciaCuervo_etal_IOC_2019.pdf.txtWilsonSavino_PatriciaCuervo_etal_IOC_2019.pdf.txtExtracted texttext/plain94926https://www.arca.fiocruz.br/bitstream/icict/35518/3/WilsonSavino_PatriciaCuervo_etal_IOC_2019.pdf.txt8bf16668ad61ef0d03c58647738d32aeMD53icict/355182023-09-05 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dc.title.pt_BR.fl_str_mv Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
title Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
spellingShingle Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
Losada-Barragán, Monica
Leishmania infantum
Timo
Microambiente tímico
Desnutrição protéica
Fluido intersticial
Leishmaniose visceral
Proteômica
Oxidação de ácidos graxos
Thymus
Thymic microenvironment
Protein malnutrition
Leishmania infantum
Visceral leishmaniasis
Interstitial fluid
Proteomics
Fatty acid oxidation
title_short Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
title_full Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
title_fullStr Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
title_full_unstemmed Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
title_sort Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
author Losada-Barragán, Monica
author_facet Losada-Barragán, Monica
Umaña-Pérez, Adriana
Durães, Jonathan
Cuervo-Escobar, Sergio
Rodríguez-Vega, Andrés
Ribeiro-Gomes, Flávia L.
Berbert, Luiz R.
Morgado, Fernanda
Porrozzi, Renato
Cruz, Daniella Arêas Mendes da
Aquino, Priscila
Carvalho, Paulo C.
Savino, Wilson
Sánchez-Gómez, Myriam
Padrón, Gabriel
Cuervo, Patricia
author_role author
author2 Umaña-Pérez, Adriana
Durães, Jonathan
Cuervo-Escobar, Sergio
Rodríguez-Vega, Andrés
Ribeiro-Gomes, Flávia L.
Berbert, Luiz R.
Morgado, Fernanda
Porrozzi, Renato
Cruz, Daniella Arêas Mendes da
Aquino, Priscila
Carvalho, Paulo C.
Savino, Wilson
Sánchez-Gómez, Myriam
Padrón, Gabriel
Cuervo, Patricia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Losada-Barragán, Monica
Umaña-Pérez, Adriana
Durães, Jonathan
Cuervo-Escobar, Sergio
Rodríguez-Vega, Andrés
Ribeiro-Gomes, Flávia L.
Berbert, Luiz R.
Morgado, Fernanda
Porrozzi, Renato
Cruz, Daniella Arêas Mendes da
Aquino, Priscila
Carvalho, Paulo C.
Savino, Wilson
Sánchez-Gómez, Myriam
Padrón, Gabriel
Cuervo, Patricia
dc.subject.other.pt_BR.fl_str_mv Leishmania infantum
Timo
Microambiente tímico
Desnutrição protéica
Fluido intersticial
Leishmaniose visceral
Proteômica
Oxidação de ácidos graxos
topic Leishmania infantum
Timo
Microambiente tímico
Desnutrição protéica
Fluido intersticial
Leishmaniose visceral
Proteômica
Oxidação de ácidos graxos
Thymus
Thymic microenvironment
Protein malnutrition
Leishmania infantum
Visceral leishmaniasis
Interstitial fluid
Proteomics
Fatty acid oxidation
dc.subject.en.pt_BR.fl_str_mv Thymus
Thymic microenvironment
Protein malnutrition
Leishmania infantum
Visceral leishmaniasis
Interstitial fluid
Proteomics
Fatty acid oxidation
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil / Universidad Antonio Nariño. Departamento de Biologia. Grupo de Investigación en Biología Celular y Funcional e Ingeniería de Biomoléculas. Bogotá, Colombia.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-12T12:55:09Z
dc.date.available.fl_str_mv 2019-09-12T12:55:09Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LOSADA-BARRAGÁN, Monica et al. Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-19, July 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35518
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2019.00252
identifier_str_mv LOSADA-BARRAGÁN, Monica et al. Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-19, July 2019.
2235-2988
10.3389/fcimb.2019.00252
url https://www.arca.fiocruz.br/handle/icict/35518
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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