Unveiling the Trypanosoma cruzi Nuclear Proteome

Detalhes bibliográficos
Autor(a) principal: Santos Júnior, Agenor de Castro Moreira dos
Data de Publicação: 2015
Outros Autores: Kalume, Dário Eluan, Camargo, Ricardo, Gómez-Mendoza, Diana Paola, Correa, José Raimundo, Chameau, Sébastien, Sousa, Marcelo Valle de, Lima, Beatriz Dolabela de, Ricart, Carlos André Ornelas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12272
Resumo: University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
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spelling Santos Júnior, Agenor de Castro Moreira dosKalume, Dário EluanCamargo, RicardoGómez-Mendoza, Diana PaolaCorrea, José RaimundoChameau, SébastienSousa, Marcelo Valle deLima, Beatriz Dolabela deRicart, Carlos André Ornelas2015-11-18T15:30:32Z2015-11-18T15:30:32Z2015SANTOS JÚNIOR, Agenor de Castro Moreira dos; et al. Unveiling the Trypanosoma cruzi Nuclear Proteome. Plos One, v.10, n.9, e0138667, 12p, Sept. 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1227210.1371/journal.pone.0138667engPlos OneUnveiling the Trypanosoma cruzi Nuclear Proteomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinas de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.Replication of Trypanosoma cruzi, the etiological agent of Chagas disease, displays peculiar features, such as absence of chromosome condensation and closed mitosis. Although previous proteome and subproteome analyses of T. cruzi have been carried out, the nuclear subproteome of this protozoan has not been described. Here, we report, for the first time to the best of our knowledge, the isolation and proteome analysis of T. cruzi nuclear fraction. For that, T. cruzi epimastigote cells were lysed and subjected to cell fractionation using two steps of sucrose density gradient centrifugation. The purity of the nuclear fraction was confirmed by phase contrast and fluorescence microscopy. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) allowed the identification of 864 proteins. Among those, 272 proteins were annotated as putative uncharacterized, and 275 had not been previously reported on global T. cruzi proteome analysis. Additionally, to support our enrichment method, bioinformatics analysis in DAVID was carried out. It grouped the nuclear proteins in 65 gene clusters, wherein the clusters with the highest enrichment scores harbor members with chromatin organization and DNA binding functions.Trypanosoma cruziChagas DiseaseNucelar ProteomeTrypanosoma cruziDoença de ChagasProteomainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12272/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALdario_kalume_etal_IOC_2015.pdfdario_kalume_etal_IOC_2015.pdfapplication/pdf1125751https://www.arca.fiocruz.br/bitstream/icict/12272/2/dario_kalume_etal_IOC_2015.pdf42d50fb57df989c4f31cc780e6cb1fc5MD52TEXTdario_kalume_etal_IOC_2015.pdf.txtdario_kalume_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Unveiling the Trypanosoma cruzi Nuclear Proteome
title Unveiling the Trypanosoma cruzi Nuclear Proteome
spellingShingle Unveiling the Trypanosoma cruzi Nuclear Proteome
Santos Júnior, Agenor de Castro Moreira dos
Trypanosoma cruzi
Chagas Disease
Nucelar Proteome
Trypanosoma cruzi
Doença de Chagas
Proteoma
title_short Unveiling the Trypanosoma cruzi Nuclear Proteome
title_full Unveiling the Trypanosoma cruzi Nuclear Proteome
title_fullStr Unveiling the Trypanosoma cruzi Nuclear Proteome
title_full_unstemmed Unveiling the Trypanosoma cruzi Nuclear Proteome
title_sort Unveiling the Trypanosoma cruzi Nuclear Proteome
author Santos Júnior, Agenor de Castro Moreira dos
author_facet Santos Júnior, Agenor de Castro Moreira dos
Kalume, Dário Eluan
Camargo, Ricardo
Gómez-Mendoza, Diana Paola
Correa, José Raimundo
Chameau, Sébastien
Sousa, Marcelo Valle de
Lima, Beatriz Dolabela de
Ricart, Carlos André Ornelas
author_role author
author2 Kalume, Dário Eluan
Camargo, Ricardo
Gómez-Mendoza, Diana Paola
Correa, José Raimundo
Chameau, Sébastien
Sousa, Marcelo Valle de
Lima, Beatriz Dolabela de
Ricart, Carlos André Ornelas
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos Júnior, Agenor de Castro Moreira dos
Kalume, Dário Eluan
Camargo, Ricardo
Gómez-Mendoza, Diana Paola
Correa, José Raimundo
Chameau, Sébastien
Sousa, Marcelo Valle de
Lima, Beatriz Dolabela de
Ricart, Carlos André Ornelas
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chagas Disease
Nucelar Proteome
topic Trypanosoma cruzi
Chagas Disease
Nucelar Proteome
Trypanosoma cruzi
Doença de Chagas
Proteoma
dc.subject.decs.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de Chagas
Proteoma
description University of Brasilia. Institute of Biology. Department of Cell Biology. Campus Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-11-18T15:30:32Z
dc.date.available.fl_str_mv 2015-11-18T15:30:32Z
dc.date.issued.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS JÚNIOR, Agenor de Castro Moreira dos; et al. Unveiling the Trypanosoma cruzi Nuclear Proteome. Plos One, v.10, n.9, e0138667, 12p, Sept. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12272
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0138667
identifier_str_mv SANTOS JÚNIOR, Agenor de Castro Moreira dos; et al. Unveiling the Trypanosoma cruzi Nuclear Proteome. Plos One, v.10, n.9, e0138667, 12p, Sept. 2015.
1932-6203
10.1371/journal.pone.0138667
url https://www.arca.fiocruz.br/handle/icict/12272
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/12272/2/dario_kalume_etal_IOC_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/12272/3/dario_kalume_etal_IOC_2015.pdf.txt
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