Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/60199 https://doi.org/10.1371/ journal.pone.0287814 |
Resumo: | Diagnostics. PATH. Seattle, Washington, USA. |
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Golden, AllisonOliveira-Silva, MichelleSlater, HannahVieira, Alexia MartinesBansil, PoojaGerth-Guyette, EmilyLeader, Brandon T.Zobrist, StephanieFerreira, Alan Kennedy BragaAraúo, Erika Crhistina Santos deCruz, Catherine Duran de LucenaGarbin, EduardoBizilj, Greg T.Carlson, Sean J.Sagalovsky, MarianaPal, SampaGupta, VinWolansky, LeoBoyle, David S.Dall’Acqua, Deusilene Souza VieiraNaveca, Felipe GomesNascimento, Valdinete Alves doSalcedo, Juan Miguel VillalobosDrain, Paul K.Costa, Alexandre Dias TavaresPereira, DhélioDomingo, Gonzalo J.2023-08-23T15:46:06Z2023-08-23T15:46:06Z2023GOLDEN, Allison et al. Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types. PLoS ONE, v. 18, n. 7, p. 1 -13, 2023.1932-6203https://www.arca.fiocruz.br/handle/icict/60199https://doi.org/10.1371/ journal.pone.0287814porPublic Library of ScienceAntigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen typesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDiagnostics. PATH. Seattle, Washington, USA.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Diagnostics. PATH. Seattle, Washington, USA.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Amazon.com. Seattle, Washington, USA.The Rockefeller Foundation. Pandemic Prevention Institute. New York, USA.Diagnostics. PATH. Seattle, Washington, USA.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Department of Global Health. University of Washington. Seattle, WA, USA. / Department of Medicine. University of Washington. Seattle, WA, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Diagnostics. PATH. Seattle, Washington, USA.The relationship between N-antigen concentration and viral load within and across different specimens guides the clinical performance of rapid diagnostic tests (RDT) in different uses. A prospective study was conducted in Porto Velho, Brazil, to investigate RDT performance in different specimen types as a function of the correlation between antigen concentration and viral load. The study included 214 close contacts with recent exposures to confirmed cases, aged 12 years and older and with various levels of vaccination. Antigen concentration was measured in nasopharyngeal swab (NPS), anterior nares swab (ANS), and saliva specimens. Reverse transcriptase (RT)–PCR was conducted on the NPS and saliva specimens, and two RDTs were conducted on ANS and one RDT on saliva. Antigen concentration correlated well with viral load when measured in the same specimen type but not across specimen types. Antigen levels were higher in symptomatic cases compared to asymptomatic/oligosymptomatic cases and lower in saliva compared to NPS and ANS samples. Discordant results between the RDTs conducted on ANS and the RT-PCR on NPS were resolved by antigen concentration values. The analytical limit of detection of RDTs can be used to predict the performance of the tests in populations for which the antigen concentration is known. The antigen dynamics across different sample types observed in SARS-CoV-2 disease progression support use of RDTs with nasal samples. Given lower antigen concentrations in saliva, rapid testing using saliva is expected to require improved RDT analytical sensitivity to achieve clinical sensitivity similar to rapid testing of nasal samples.Rapid Diagnostic TestsSampling StudiesAntigensPrueba de Diagnóstico RápidoMuestreoAntígenosÉtudes par échantillonnageAntigènesSARS-CoV-2Testes de Diagnóstico RápidoAmostragemAntígenosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60199/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjournal.pone.Antigenok.pdfjournal.pone.Antigenok.pdfapplication/pdf2534735https://www.arca.fiocruz.br/bitstream/icict/60199/2/journal.pone.Antigenok.pdfbd961651729bbd0fefc1a7d2411db3fbMD52icict/601992023-08-23 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| dc.title.en_US.fl_str_mv |
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types |
| title |
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types |
| spellingShingle |
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types Golden, Allison Rapid Diagnostic Tests Sampling Studies Antigens Prueba de Diagnóstico Rápido Muestreo Antígenos Études par échantillonnage Antigènes SARS-CoV-2 Testes de Diagnóstico Rápido Amostragem Antígenos |
| title_short |
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types |
| title_full |
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types |
| title_fullStr |
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types |
| title_full_unstemmed |
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types |
| title_sort |
Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types |
| author |
Golden, Allison |
| author_facet |
Golden, Allison Oliveira-Silva, Michelle Slater, Hannah Vieira, Alexia Martines Bansil, Pooja Gerth-Guyette, Emily Leader, Brandon T. Zobrist, Stephanie Ferreira, Alan Kennedy Braga Araúo, Erika Crhistina Santos de Cruz, Catherine Duran de Lucena Garbin, Eduardo Bizilj, Greg T. Carlson, Sean J. Sagalovsky, Mariana Pal, Sampa Gupta, Vin Wolansky, Leo Boyle, David S. Dall’Acqua, Deusilene Souza Vieira Naveca, Felipe Gomes Nascimento, Valdinete Alves do Salcedo, Juan Miguel Villalobos Drain, Paul K. Costa, Alexandre Dias Tavares Pereira, Dhélio Domingo, Gonzalo J. |
| author_role |
author |
| author2 |
Oliveira-Silva, Michelle Slater, Hannah Vieira, Alexia Martines Bansil, Pooja Gerth-Guyette, Emily Leader, Brandon T. Zobrist, Stephanie Ferreira, Alan Kennedy Braga Araúo, Erika Crhistina Santos de Cruz, Catherine Duran de Lucena Garbin, Eduardo Bizilj, Greg T. Carlson, Sean J. Sagalovsky, Mariana Pal, Sampa Gupta, Vin Wolansky, Leo Boyle, David S. Dall’Acqua, Deusilene Souza Vieira Naveca, Felipe Gomes Nascimento, Valdinete Alves do Salcedo, Juan Miguel Villalobos Drain, Paul K. Costa, Alexandre Dias Tavares Pereira, Dhélio Domingo, Gonzalo J. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Golden, Allison Oliveira-Silva, Michelle Slater, Hannah Vieira, Alexia Martines Bansil, Pooja Gerth-Guyette, Emily Leader, Brandon T. Zobrist, Stephanie Ferreira, Alan Kennedy Braga Araúo, Erika Crhistina Santos de Cruz, Catherine Duran de Lucena Garbin, Eduardo Bizilj, Greg T. Carlson, Sean J. Sagalovsky, Mariana Pal, Sampa Gupta, Vin Wolansky, Leo Boyle, David S. Dall’Acqua, Deusilene Souza Vieira Naveca, Felipe Gomes Nascimento, Valdinete Alves do Salcedo, Juan Miguel Villalobos Drain, Paul K. Costa, Alexandre Dias Tavares Pereira, Dhélio Domingo, Gonzalo J. |
| dc.subject.en.en_US.fl_str_mv |
Rapid Diagnostic Tests Sampling Studies Antigens |
| topic |
Rapid Diagnostic Tests Sampling Studies Antigens Prueba de Diagnóstico Rápido Muestreo Antígenos Études par échantillonnage Antigènes SARS-CoV-2 Testes de Diagnóstico Rápido Amostragem Antígenos |
| dc.subject.es.en_US.fl_str_mv |
Prueba de Diagnóstico Rápido Muestreo Antígenos |
| dc.subject.fr.en_US.fl_str_mv |
Études par échantillonnage Antigènes |
| dc.subject.decs.en_US.fl_str_mv |
SARS-CoV-2 Testes de Diagnóstico Rápido Amostragem Antígenos |
| description |
Diagnostics. PATH. Seattle, Washington, USA. |
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2023 |
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2023-08-23T15:46:06Z |
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2023-08-23T15:46:06Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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GOLDEN, Allison et al. Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types. PLoS ONE, v. 18, n. 7, p. 1 -13, 2023. |
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https://www.arca.fiocruz.br/handle/icict/60199 |
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1932-6203 |
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https://doi.org/10.1371/ journal.pone.0287814 |
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GOLDEN, Allison et al. Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types. PLoS ONE, v. 18, n. 7, p. 1 -13, 2023. 1932-6203 |
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https://www.arca.fiocruz.br/handle/icict/60199 https://doi.org/10.1371/ journal.pone.0287814 |
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Public Library of Science |
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Public Library of Science |
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