CRISPR-assisted test for Schistosoma haematobium

Detalhes bibliográficos
Autor(a) principal: Cherkaoui, Dounia
Data de Publicação: 2023
Outros Autores: Mesquita, Silvia Gonçalves, Huang, Da, Lugli, Elena B, Webster, Bonnie L, McKendry, Rachel A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57892
Resumo: London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK.
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spelling Cherkaoui, DouniaMesquita, Silvia GonçalvesHuang, DaLugli, Elena BWebster, Bonnie LMcKendry, Rachel A2023-04-19T17:58:10Z2023-04-19T17:58:10Z2023CHERKAOUI, Dounia et al. CRISPR-assisted test for Schistosoma haematobium. Sci Rep, 13, n. 1, 4990, 2023. doi: 10.1038/s41598-023-31238-y.2045-2322https://www.arca.fiocruz.br/handle/icict/57892engNature Publishing GroupCRISPR-assisted test for Schistosoma haematobiuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondon Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK.Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK/René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK.Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.Wolfson Wellcome Biomedical Laboratories. Department of Science. Natural History Museum. Cromwell Road, London, SW7 5BD, UK/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK.Schistosomiasis is a major neglected tropical disease targeted for elimination as a public health issue by 2030, however there is an urgent need for more sensitive and specific diagnostic tests suitable to resource-limited settings. Here we developed CATSH, a CRISPR-assisted diagnostic test for Schistosoma haematobium, utilising recombinase polymerase amplification, Cas12a-targeted cleavage and portable real-time fluorescence detection. CATSH showed high analytical sensitivity, consistent detection of a single parasitic egg and specificity for urogenital Schistosoma species. Thanks to a novel CRISPR-compatible sample preparation developed using simulated urine samples containing parasitic eggs, CATSH had a sample-to-result within 2 h. The components of CATSH can be lyophilised, reducing cold chain dependence and widening access to lower and middle-income countries. This work presents a new application of CRISPR diagnostics for highly sensitive and specific detection of parasitic pathogens in remote areas and could have a significant impact on the elimination of neglected tropical diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57892/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCRISPR-assisted test for Schistosoma haematobium.pdfCRISPR-assisted test for Schistosoma haematobium.pdfapplication/pdf1632281https://www.arca.fiocruz.br/bitstream/icict/57892/2/CRISPR-assisted%20test%20for%20Schistosoma%20haematobium.pdf8bb1319bba8a284e56cbb1d544ffe77cMD52icict/578922023-04-19 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dc.title.en_US.fl_str_mv CRISPR-assisted test for Schistosoma haematobium
title CRISPR-assisted test for Schistosoma haematobium
spellingShingle CRISPR-assisted test for Schistosoma haematobium
Cherkaoui, Dounia
title_short CRISPR-assisted test for Schistosoma haematobium
title_full CRISPR-assisted test for Schistosoma haematobium
title_fullStr CRISPR-assisted test for Schistosoma haematobium
title_full_unstemmed CRISPR-assisted test for Schistosoma haematobium
title_sort CRISPR-assisted test for Schistosoma haematobium
author Cherkaoui, Dounia
author_facet Cherkaoui, Dounia
Mesquita, Silvia Gonçalves
Huang, Da
Lugli, Elena B
Webster, Bonnie L
McKendry, Rachel A
author_role author
author2 Mesquita, Silvia Gonçalves
Huang, Da
Lugli, Elena B
Webster, Bonnie L
McKendry, Rachel A
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cherkaoui, Dounia
Mesquita, Silvia Gonçalves
Huang, Da
Lugli, Elena B
Webster, Bonnie L
McKendry, Rachel A
description London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-04-19T17:58:10Z
dc.date.available.fl_str_mv 2023-04-19T17:58:10Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CHERKAOUI, Dounia et al. CRISPR-assisted test for Schistosoma haematobium. Sci Rep, 13, n. 1, 4990, 2023. doi: 10.1038/s41598-023-31238-y.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57892
dc.identifier.issn.en_US.fl_str_mv 2045-2322
identifier_str_mv CHERKAOUI, Dounia et al. CRISPR-assisted test for Schistosoma haematobium. Sci Rep, 13, n. 1, 4990, 2023. doi: 10.1038/s41598-023-31238-y.
2045-2322
url https://www.arca.fiocruz.br/handle/icict/57892
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Nature Publishing Group
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/57892/2/CRISPR-assisted%20test%20for%20Schistosoma%20haematobium.pdf
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