Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes

Carnielli, Juliana B. T; Andrade, Hélida M. de; Pìres, Simone F; Chapeaurouge, Alexander D; Perales, Jonas; Rocha, Renata Monti; Carvalho, Silvio F. G.; Ribeiro, Leonardo P; Dietze, Reynaldo; Figueiredo, Suely G; Lemos, Elenice M

Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes

Detalhes bibliográficos
Autor(a) principal:	Carnielli, Juliana B. T
Data de Publicação:	2014
Outros Autores:	Andrade, Hélida M. de, Pìres, Simone F, Chapeaurouge, Alexander D, Perales, Jonas, Rocha, Renata Monti, Carvalho, Silvio F. G., Ribeiro, Leonardo P, Dietze, Reynaldo, Figueiredo, Suely G, Lemos, Elenice M
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/10978
Resumo:	Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Departamento de Ciências Fisiológicas. Laboratório de Química de Proteínas. Vitória, ES, Brasil.

Metadados do item

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spelling	Carnielli, Juliana B. TAndrade, Hélida M. dePìres, Simone FChapeaurouge, Alexander DPerales, JonasRocha, Renata MontiCarvalho, Silvio F. G.Ribeiro, Leonardo PDietze, ReynaldoFigueiredo, Suely GLemos, Elenice M2015-06-26T13:04:36Z2015-06-26T13:04:36Z2014CARNIELLI, Juliana B. T. et al. Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes. Journal of Proteomics, v.108, p.198-208, aug. 2014.1874-3919https://www.arca.fiocruz.br/handle/icict/1097810.1016/j.jprot.2014.05.010engElsevierProteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Departamento de Ciências Fisiológicas. Laboratório de Química de Proteínas. Vitória, ES, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Leishmanioses. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Leishmanioses. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES. Brasil.Universidade Estadual de Montes Claros. Hospital Universitário Clemente de Faria. Montes Claros, MG, Brasil.Universidade Federal do Espírito Santo. Departamento de Ciências Fisiológicas. Laboratório de Química de Proteínas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES. Brasil.Universidade Federal do Espírito Santo. Departamento de Ciências Fisiológicas. Laboratório de Química de Proteínas. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES. Brasil.The mechanism of miltefosine-resistance in Leishmania spp. has been partially determined in experimental resistant lines; however, studies using clinical isolates with different miltefosine susceptibilities are still needed. In our study, we used a proteomic 2D-DIGE/MS approach to study different protein abundances in miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates from visceral leishmaniasis patients with different miltefosine treatment outcomes. The high-resolution proteome obtained from these isolates showed 823 matched spots and 46 spots exhibited different abundances between the isolates. Out of these differentially expressed spots, 26 (56.5%) showed greater and 20 (43.5%) showed lower expression of the resistant isolate compared to the sensitive isolate. MALDI/TOF–TOF mass spectrometry allowed the identification of 32 spots with unique protein identification correspondent to 22 non-redundant proteins. Most of the proteins up-regulated in the proteome miltefosine-resistant isolates were associated with redox homeostasis, stress response, protection to apoptosis, and drug translocation. These differentially expressed proteins are likely involved in miltefosine natural resistance and suggest that the miltefosine-resistance mechanism in Leishmania is multifactorial. Biological significance Visceral leishmaniasis (VL) is a serious disease with a challenging treatment plan requiring the prolonged and painful applications of poorly tolerated toxic drugs. Therefore, the identification of miltefosine, an effective and safe oral drug, was considered a significant advancement in leishmaniasis therapy. However, different sensitivities to miltefosine in Leishmania have been observed in clinically relevant species, and the biological mechanism by which clinical isolates of Leishmania acquire drug resistance is poorly understood. Our work aims to elucidate the mechanism of natural resistance to miltefosine in Leishmania by studying the isolates from VL patients who displayed different miltefosine treatment outcomes.Leishmania infantum chagasiBrazilTreatmentProteômicaLeishmania infantuminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10978/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALalexaner_chapeaurougeetal_IOC_2014.pdfapplication/pdf818523https://www.arca.fiocruz.br/bitstream/icict/10978/2/alexaner_chapeaurougeetal_IOC_2014.pdfe87c9b729c84a36d7b775e4cd4c8781eMD52TEXTalexaner_chapeaurougeetal_IOC_2014.pdf.txtalexaner_chapeaurougeetal_IOC_2014.pdf.txtExtracted texttext/plain52730https://www.arca.fiocruz.br/bitstream/icict/10978/3/alexaner_chapeaurougeetal_IOC_2014.pdf.txtc24ee41f30264673c8f6f07f01e47c7bMD53icict/109782023-09-05 10:22:59.553oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:22:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes
title	Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes
spellingShingle	Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes Carnielli, Juliana B. T Leishmania infantum chagasi Brazil Treatment Proteômica Leishmania infantum
title_short	Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes
title_full	Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes
title_fullStr	Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes
title_full_unstemmed	Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes
title_sort	Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes
author	Carnielli, Juliana B. T
author_facet	Carnielli, Juliana B. T Andrade, Hélida M. de Pìres, Simone F Chapeaurouge, Alexander D Perales, Jonas Rocha, Renata Monti Carvalho, Silvio F. G. Ribeiro, Leonardo P Dietze, Reynaldo Figueiredo, Suely G Lemos, Elenice M
author_role	author
author2	Andrade, Hélida M. de Pìres, Simone F Chapeaurouge, Alexander D Perales, Jonas Rocha, Renata Monti Carvalho, Silvio F. G. Ribeiro, Leonardo P Dietze, Reynaldo Figueiredo, Suely G Lemos, Elenice M
author2_role	author author author author author author author author author author
dc.contributor.author.fl_str_mv	Carnielli, Juliana B. T Andrade, Hélida M. de Pìres, Simone F Chapeaurouge, Alexander D Perales, Jonas Rocha, Renata Monti Carvalho, Silvio F. G. Ribeiro, Leonardo P Dietze, Reynaldo Figueiredo, Suely G Lemos, Elenice M
dc.subject.en.pt_BR.fl_str_mv	Leishmania infantum chagasi Brazil Treatment
topic	Leishmania infantum chagasi Brazil Treatment Proteômica Leishmania infantum
dc.subject.decs.pt_BR.fl_str_mv	Proteômica Leishmania infantum
description	Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Departamento de Ciências Fisiológicas. Laboratório de Química de Proteínas. Vitória, ES, Brasil.
publishDate	2014
dc.date.issued.fl_str_mv	2014
dc.date.accessioned.fl_str_mv	2015-06-26T13:04:36Z
dc.date.available.fl_str_mv	2015-06-26T13:04:36Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	CARNIELLI, Juliana B. T. et al. Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes. Journal of Proteomics, v.108, p.198-208, aug. 2014.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/10978
dc.identifier.issn.pt_BR.fl_str_mv	1874-3919
dc.identifier.doi.pt_BR.fl_str_mv	10.1016/j.jprot.2014.05.010
identifier_str_mv	CARNIELLI, Juliana B. T. et al. Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes. Journal of Proteomics, v.108, p.198-208, aug. 2014. 1874-3919 10.1016/j.jprot.2014.05.010
url	https://www.arca.fiocruz.br/handle/icict/10978
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes

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