Trypanosoma cruzi transcriptome during axenic epimastigote growth curve

Detalhes bibliográficos
Autor(a) principal: Santos, Cyndia Mara Bezerra dos
Data de Publicação: 2018
Outros Autores: Ludwig, Adriana, Kessler, Rafael Luis, Rampazzo, Rita de Cássia Pontello, Inoue, Alexandre Haruo, Krieger, Marco Aurélio, Pavoni, Daniela Parada, Probst, Christian Macagnan
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31652
Resumo: Universidade Federal do Paraná. Centro Politécnico. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil.
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spelling Santos, Cyndia Mara Bezerra dosLudwig, AdrianaKessler, Rafael LuisRampazzo, Rita de Cássia PontelloInoue, Alexandre HaruoKrieger, Marco AurélioPavoni, Daniela ParadaProbst, Christian Macagnan2019-02-14T17:36:01Z2019-02-14T17:36:01Z2018SANTOS, Cyndia Mara Bezerra dos et al. Trypanosoma cruzi transcriptome during axenic epimastigote growth curve. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 5, p. 1-13, 2018.1678-8060https://www.arca.fiocruz.br/handle/icict/3165210.1590/0074-02760170404porFundação Oswaldo Cruz. Instituto Oswaldo CruzGrânulos de RNAAnálise TranscriptômicaChagas DiseaseGrowth ChartsTranscriptomics AnalysisRNA SequencePolyribosomesDoença de ChagasGráficos de CrescimentoSequência de BasesPolirribossomosTrypanosoma cruzi transcriptome during axenic epimastigote growth curveinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Centro Politécnico. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Universidade Federal do Paraná. Centro Politécnico. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Universidade Federal do Paraná. Centro Politécnico. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil.Universidade Federal do Paraná. Centro Politécnico. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil.Trypanosoma cruzi is an important protozoan parasite and the causative agent of Chagas disease. A critical step in understanding T. cruzi biology is the study of cellular and molecular features exhibited during its growth curve. We aimed to acquire a global view of the gene expression profile of T. cruzi during epimastigote growth. RNA-Seq analysis of total and polysomal/granular RNA fractions was performed along the 10 days T. cruzi epimastigote growth curve in vitro, in addition to cell viability and cell cycle analyses. We also analysed the polysome profile and investigated the presence of granular RNA by FISH and western blotting. We identified 1082 differentially expressed genes (DEGs), of which 220 were modulated in both fractions. According to the modulation pattern, DEGs were grouped into 12 clusters and showed enrichment of important gene ontology (GO) terms. Moreover, we showed that by the sixth day of the growth curve, polysomal content declined greatly and the RNA granules content appeared to increase, suggesting that a portion of mRNAs isolated from the sucrose gradient during late growth stages was associated with RNA granules and not only polyribosomes. Furthermore, we discuss several modulated genes possibly involved in T. cruzi growth, mainly during the stationary phase, such as genes related to cell cycle, pathogenesis, metabolic processes and RNA-binding proteins.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/31652/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL0074-0276-mioc-113-5.pdf0074-0276-mioc-113-5.pdfapplication/pdf3046522https://www.arca.fiocruz.br/bitstream/icict/31652/2/0074-0276-mioc-113-5.pdfca70ac4a197b74c287651a9f0359a489MD52TEXT0074-0276-mioc-113-5.pdf.txt0074-0276-mioc-113-5.pdf.txtExtracted texttext/plain55244https://www.arca.fiocruz.br/bitstream/icict/31652/3/0074-0276-mioc-113-5.pdf.txtbfbbde07300c103f454cc0cf982b3835MD53icict/316522019-02-15 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dc.title.pt_BR.fl_str_mv Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
title Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
spellingShingle Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
Santos, Cyndia Mara Bezerra dos
Grânulos de RNA
Análise Transcriptômica
Chagas Disease
Growth Charts
Transcriptomics Analysis
RNA Sequence
Polyribosomes
Doença de Chagas
Gráficos de Crescimento
Sequência de Bases
Polirribossomos
title_short Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
title_full Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
title_fullStr Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
title_full_unstemmed Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
title_sort Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
author Santos, Cyndia Mara Bezerra dos
author_facet Santos, Cyndia Mara Bezerra dos
Ludwig, Adriana
Kessler, Rafael Luis
Rampazzo, Rita de Cássia Pontello
Inoue, Alexandre Haruo
Krieger, Marco Aurélio
Pavoni, Daniela Parada
Probst, Christian Macagnan
author_role author
author2 Ludwig, Adriana
Kessler, Rafael Luis
Rampazzo, Rita de Cássia Pontello
Inoue, Alexandre Haruo
Krieger, Marco Aurélio
Pavoni, Daniela Parada
Probst, Christian Macagnan
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Cyndia Mara Bezerra dos
Ludwig, Adriana
Kessler, Rafael Luis
Rampazzo, Rita de Cássia Pontello
Inoue, Alexandre Haruo
Krieger, Marco Aurélio
Pavoni, Daniela Parada
Probst, Christian Macagnan
dc.subject.other.pt_BR.fl_str_mv Grânulos de RNA
Análise Transcriptômica
topic Grânulos de RNA
Análise Transcriptômica
Chagas Disease
Growth Charts
Transcriptomics Analysis
RNA Sequence
Polyribosomes
Doença de Chagas
Gráficos de Crescimento
Sequência de Bases
Polirribossomos
dc.subject.en.pt_BR.fl_str_mv Chagas Disease
Growth Charts
Transcriptomics Analysis
RNA Sequence
Polyribosomes
dc.subject.decs.pt_BR.fl_str_mv Doença de Chagas
Gráficos de Crescimento
Sequência de Bases
Polirribossomos
description Universidade Federal do Paraná. Centro Politécnico. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-14T17:36:01Z
dc.date.available.fl_str_mv 2019-02-14T17:36:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Cyndia Mara Bezerra dos et al. Trypanosoma cruzi transcriptome during axenic epimastigote growth curve. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 5, p. 1-13, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31652
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760170404
identifier_str_mv SANTOS, Cyndia Mara Bezerra dos et al. Trypanosoma cruzi transcriptome during axenic epimastigote growth curve. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 5, p. 1-13, 2018.
1678-8060
10.1590/0074-02760170404
url https://www.arca.fiocruz.br/handle/icict/31652
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31652/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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