Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides

Detalhes bibliográficos
Autor(a) principal: Pérez, Cindy
Data de Publicação: 2021
Outros Autores: Díaz-Roa, Andrea, Bernal, Yuli, Arenas, Nelson E., Kalume, Dario Eluan, Côrtes, Luzia Monteiro de Castro, Silva Junior, Pedro I. da, Varela, Yahson, Patarroyo, Manuel A., Torres, Orlando, Bello, Felio J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/48253
Resumo: Universidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia.
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spelling Pérez, CindyDíaz-Roa, AndreaBernal, YuliArenas, Nelson E.Kalume, Dario EluanCôrtes, Luzia Monteiro de CastroSilva Junior, Pedro I. daVarela, YahsonPatarroyo, Manuel A.Torres, OrlandoBello, Felio J.2021-07-16T12:43:21Z2021-07-16T12:43:21Z2021PÉREZ, Cindy et al. Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200587, 11 p, 2021.0074-0276https://www.arca.fiocruz.br/handle/icict/4825310.1590/0074-02760200587engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Sarconesiopsis magellanicaPeptídeos antimicrobianosCorpo de gordura larvalCaracterização físico-químicaAvaliação antibacterianaSarconesiopsis magellanicaAntimicrobial peptidesLarval fat bodyPhysicochemical characterisationAntibacterial evaluationCharacterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia.Instituto Butantan, Laboratório de Toxinología Aplicada. São Paulo, SP, Brasil / Universidad Nacional Abierta y a Distancia, Escuela de Ciencias Agrícolas, Pecuarias y de Medio Ambiente. Bogotá, Colombia.Universidad Antonio Nariño, Facultad de Ciencias. Bogotá, Colombia.Universidad Antonio Nariño, Facultad de Ciencias. Bogotá, Colombia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Instituto Butantan, Laboratório de Toxinología Aplicada. São Paulo, SP, Brasil.Fundación Instituto de Inmunología de Colombia, Molecular Biology and Immunology Department. Bogotá, Colombia.Fundación Instituto de Inmunología de Colombia, Molecular Biology and Immunology Department. Bogotá, Colombia / Universidad Nacional de Colombia, Faculty of Medicine, Microbiology Department, Bogotá, Colombia / Universidad Santo Tomás, Health Sciences Division. Bogotá, Colombia.Universidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia.Universidad de La Salle, Facultad de Ciencias Agropecuarias, Programa de Medicina Veterinaria. Bogotá, Colombia.BACKGROUND The inappropriate use of antibiotics has led to the accelerated growth of resistance to antibiotics. The search for new therapeutic strategies (i.e., antimicrobial peptides-AMPs) has thus become a pressing need. OBJECTIVE Characterising and evaluating Sarconesiopsis magellanica larval fat body-derived AMPs. METHODS Fat body extracts were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC); mass spectrometry was used for characterising the primary structure of the AMPs so found. ProtParam (Expasy) was used for analysing the AMPs’ physico-chemical properties. Synthetic AMPs’ antibacterial activity was evaluated. FINDINGS Four new AMPs were obtained and called sarconesin III, IV, V and VI. Sarconesin III had an α-helix structure and sarconesins IV, V and VI had linear formations. Oligomer prediction highlighted peptide-peptide interactions, suggesting that sarconesins III, V and VI could form self-aggregations when in contact with the microbial membrane. AMPs synthesised from their native molecules’ sequences had potent activity against Gram-positive bacteria and, to a lesser extent, against Gram-negative and drug-resistant bacteria. Sarconesin VI was the most efficient AMP. None of the four synthetic AMPs had a cytotoxic effect. MAIN CONCLUSIONS S. magellanica larval fat body-derived antimicrobial peptides are an important source of AMPs and could be used in different antimicrobial therapies and overcoming bacterial resistance,info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48253/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDarioKalume_LuisaCortes_etal_IOC_1921.pdfDarioKalume_LuisaCortes_etal_IOC_1921.pdfapplication/pdf2001336https://www.arca.fiocruz.br/bitstream/icict/48253/2/DarioKalume_LuisaCortes_etal_IOC_1921.pdfc19ad1b1cdb5730344789bf84ab842d2MD52TEXTDarioKalume_LuisaCortes_etal_IOC_1921.pdf.txtDarioKalume_LuisaCortes_etal_IOC_1921.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
title Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
spellingShingle Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
Pérez, Cindy
Sarconesiopsis magellanica
Peptídeos antimicrobianos
Corpo de gordura larval
Caracterização físico-química
Avaliação antibacteriana
Sarconesiopsis magellanica
Antimicrobial peptides
Larval fat body
Physicochemical characterisation
Antibacterial evaluation
title_short Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
title_full Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
title_fullStr Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
title_full_unstemmed Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
title_sort Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides
author Pérez, Cindy
author_facet Pérez, Cindy
Díaz-Roa, Andrea
Bernal, Yuli
Arenas, Nelson E.
Kalume, Dario Eluan
Côrtes, Luzia Monteiro de Castro
Silva Junior, Pedro I. da
Varela, Yahson
Patarroyo, Manuel A.
Torres, Orlando
Bello, Felio J.
author_role author
author2 Díaz-Roa, Andrea
Bernal, Yuli
Arenas, Nelson E.
Kalume, Dario Eluan
Côrtes, Luzia Monteiro de Castro
Silva Junior, Pedro I. da
Varela, Yahson
Patarroyo, Manuel A.
Torres, Orlando
Bello, Felio J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pérez, Cindy
Díaz-Roa, Andrea
Bernal, Yuli
Arenas, Nelson E.
Kalume, Dario Eluan
Côrtes, Luzia Monteiro de Castro
Silva Junior, Pedro I. da
Varela, Yahson
Patarroyo, Manuel A.
Torres, Orlando
Bello, Felio J.
dc.subject.other.pt_BR.fl_str_mv Sarconesiopsis magellanica
Peptídeos antimicrobianos
Corpo de gordura larval
Caracterização físico-química
Avaliação antibacteriana
topic Sarconesiopsis magellanica
Peptídeos antimicrobianos
Corpo de gordura larval
Caracterização físico-química
Avaliação antibacteriana
Sarconesiopsis magellanica
Antimicrobial peptides
Larval fat body
Physicochemical characterisation
Antibacterial evaluation
dc.subject.en.pt_BR.fl_str_mv Sarconesiopsis magellanica
Antimicrobial peptides
Larval fat body
Physicochemical characterisation
Antibacterial evaluation
description Universidad Antonio Nariño, Facultad de Medicina Veterinaria. Bogotá, Colombia.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-07-16T12:43:21Z
dc.date.available.fl_str_mv 2021-07-16T12:43:21Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PÉREZ, Cindy et al. Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200587, 11 p, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/48253
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760200587
identifier_str_mv PÉREZ, Cindy et al. Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e200587, 11 p, 2021.
0074-0276
10.1590/0074-02760200587
url https://www.arca.fiocruz.br/handle/icict/48253
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/48253/2/DarioKalume_LuisaCortes_etal_IOC_1921.pdf
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