Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity

Detalhes bibliográficos
Autor(a) principal: Mendanha, Sebastião A.
Data de Publicação: 2013
Outros Autores: Moura, Soraia S., Anjos, Jorge L. V., Valadares, Marize C., Alonso, Antonio A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36036
Resumo: Soraia S. Moura. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
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spelling Mendanha, Sebastião A.Moura, Soraia S.Anjos, Jorge L. V.Valadares, Marize C.Alonso, Antonio A.2019-10-01T14:11:47Z2019-10-01T14:11:47Z2013MENDANHA, Sebastião A. et al. Toxicity of terpenes on fibroblast cells compared to their hemolytic potentialand increase in erythrocyte membrane fluidity. Toxicology in Vitro, v. 27, p. 323-329, 2013.0887-2333https://www.arca.fiocruz.br/handle/icict/3603610.1016/j.tiv.2012.08.0221879-3177Soraia S. Moura. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.2020-10-01Universidade Federal de Goiás. Instituto de Física. Goiânia, GO, Brasil.Universidade Federal de Goiás. Faculdade de Farmácia. Laboratório de Farmacologia e Toxicologia Celular. Goiânia, GO, Brasil.Universidade Federal de Goiás. Instituto de Física. Goiânia, GO, Brasil.Universidade Federal de Goiás. Faculdade de Farmácia. Laboratório de Farmacologia e Toxicologia Celular. Goiânia, GO, Brasil.Universidade Federal de Goiás. Instituto de Física. Goiânia, GO, Brasil.erpenes are considered potent skin permeation enhancers with low toxicity. Electron paramagnetic res-onance (EPR) spectroscopy of the spin label 5-doxyl stearic acid (5-DSA) was used to monitor the effect ofsesquiterpene nerolidol and various monoterpenes on membrane fluidity in erythrocyte and fibroblastcells. In addition, the hemolytic levels and cytotoxic effects on cultured fibroblast cells were also mea-sured to investigate possible relationships between the cellular irritation potentials of terpenes andthe ability to modify membrane fluidity. All terpenes increased cell membrane fluidity with no significantdifferences between the monoterpenes, but the effect of sesquiterpene was significantly greater than thatof the monoterpenes. The IC50values for the terpenes in the cytotoxicity assay indicated that 1,8-cineoleshowed lower cytotoxicity anda-terpineol and nerolidol showed higher cytotoxicity. The correlationbetween the hemolytic effect and the IC50values for fibroblast viability was low (R= 0.61); however,in both tests, nerolidol was among the most aggressive of terpenes and 1,8-cineole was among the leastaggressive. Obtaining information concerning the toxicity and potency of terpenes could aid in the designof topical formulations optimized to facilitate drug absorption for the treatment of many skin diseases.engElsevierToxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEPRSpin labelToxicityFibroblastTerpenesErythrocyte membraneinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36036/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Mendanha_Sebastião_etal_INI_2013.pdfve_Mendanha_Sebastião_etal_INI_2013.pdfapplication/pdf497115https://www.arca.fiocruz.br/bitstream/icict/36036/2/ve_Mendanha_Sebasti%c3%a3o_etal_INI_2013.pdff3278ea948e681ede032a954b19ca03dMD52TEXTve_Mendanha_Sebastião_etal_INI_2013.pdf.txtve_Mendanha_Sebastião_etal_INI_2013.pdf.txtExtracted texttext/plain36776https://www.arca.fiocruz.br/bitstream/icict/36036/3/ve_Mendanha_Sebasti%c3%a3o_etal_INI_2013.pdf.txt7e6e4302dbd541535e1c219d84c010fbMD53icict/360362021-03-24 16:32:36.94oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
title Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
spellingShingle Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
Mendanha, Sebastião A.
EPR
Spin label
Toxicity
Fibroblast
Terpenes
Erythrocyte membrane
title_short Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
title_full Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
title_fullStr Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
title_full_unstemmed Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
title_sort Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
author Mendanha, Sebastião A.
author_facet Mendanha, Sebastião A.
Moura, Soraia S.
Anjos, Jorge L. V.
Valadares, Marize C.
Alonso, Antonio A.
author_role author
author2 Moura, Soraia S.
Anjos, Jorge L. V.
Valadares, Marize C.
Alonso, Antonio A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mendanha, Sebastião A.
Moura, Soraia S.
Anjos, Jorge L. V.
Valadares, Marize C.
Alonso, Antonio A.
dc.subject.en.pt_BR.fl_str_mv EPR
Spin label
Toxicity
Fibroblast
Terpenes
Erythrocyte membrane
topic EPR
Spin label
Toxicity
Fibroblast
Terpenes
Erythrocyte membrane
description Soraia S. Moura. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2019-10-01T14:11:47Z
dc.date.available.fl_str_mv 2019-10-01T14:11:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MENDANHA, Sebastião A. et al. Toxicity of terpenes on fibroblast cells compared to their hemolytic potentialand increase in erythrocyte membrane fluidity. Toxicology in Vitro, v. 27, p. 323-329, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36036
dc.identifier.issn.pt_BR.fl_str_mv 0887-2333
dc.identifier.doi.none.fl_str_mv 10.1016/j.tiv.2012.08.022
dc.identifier.eissn.none.fl_str_mv 1879-3177
identifier_str_mv MENDANHA, Sebastião A. et al. Toxicity of terpenes on fibroblast cells compared to their hemolytic potentialand increase in erythrocyte membrane fluidity. Toxicology in Vitro, v. 27, p. 323-329, 2013.
0887-2333
10.1016/j.tiv.2012.08.022
1879-3177
url https://www.arca.fiocruz.br/handle/icict/36036
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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