Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion

Detalhes bibliográficos
Autor(a) principal: Magalhães, Kelly G.
Data de Publicação: 2018
Outros Autores: Gomes, Tatiana Luna, Santos, Fabio Mesquita, Corrêa, Rafael, Assunção, Leonardo Santos, Atella, Georgia Correa, Weller, Peter F., Melo, Christianne Bandeira, Bozza, Patrícia T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32839
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil / Universidade de Brasília. Laboratório de Imunologia e Inflamação. Brasília, DF, Brasil.
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spelling Magalhães, Kelly G.Gomes, Tatiana LunaSantos, Fabio MesquitaCorrêa, RafaelAssunção, Leonardo SantosAtella, Georgia CorreaWeller, Peter F.Melo, Christianne BandeiraBozza, Patrícia T.2019-04-29T16:03:00Z2019-04-29T16:03:00Z2018MAGALHÃES, Kelly G. et al. Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion. Frontiers in Immunology, v. 9. p.1-11, Jan. 2019.1664-3224https://www.arca.fiocruz.br/handle/icict/3283910.3389/fimmu.2018.03161engFrontiers MediaEosinófilosLipídeosSchistosomaEosinophilPGD2LipidsSchistosomaTLRLPCTGFbEoxinProstaglandina D2Fator 88 de Diferenciação MieloideSchistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil / Universidade de Brasília. Laboratório de Imunologia e Inflamação. Brasília, DF, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Instituto de Aplicação Fernando Rodrigues da Silveira. Departamento de Ciências da Natureza. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Centro Universitário da Zona Oeste. Unidade de Farmácia. Laboratório de Pesquisas em Análise Clínicas. Rio de Janeiro, RJ, Brasil.Universidade de Brasília. Laboratório de Imunologia e Inflamação. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Bioquímica de Lipídeos e Lipoproteínas. Rio de Janeiro, RJ, Brasil.Harvard Medical School. Allergy and Inflammation. Boston, MA, USA.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Parasite-derived lipids may play important roles in host-pathogen interactions and immune evasion mechanisms. Remarkable accumulation of eosinophils is a characteristic feature of inflammation associated with parasitic disease, especially caused by helminthes. Infiltrating eosinophils are implicated in the pathogenesis of helminth infection by virtue of their capacity to release an array of tissue-damaging and immunoregulatory mediators. However, the mechanisms involved in the activation of human eosinophils by parasite-derived molecules are not clear. Here we investigated the effects and mechanisms of schistosomal lipids-induced activation of human eosinophils. Our results showed that stimulation of human eosinophils in vitro with total lipid extracts from adult worms of S. mansoni induced direct activation of human eosinophils, eliciting lipid droplet biogenesis, synthesis of leukotriene (LT) C4 and eoxin (EX) C4 (14,15 LTC4) and secretion of eosinophil pre-formed TGFβ. We demonstrated that main eosinophil activating components within S. mansoni lipid extract are schistosomal-derived lysophosphatidylcholine (LPC) and prostaglandin (PG)D2. Moreover, TLR2 is up-regulated in human eosinophils upon stimulation with schistosomal lipids and pre-treatment with anti-TLR2 inhibited both schistosomal lipids- and LPC-, but not PGD2-, induced lipid droplet biogenesis and EXC4 synthesis within eosinophils, indicating that TLR2 mediates LPC-driven human eosinophil activation. By employing PGD2 receptor antagonists, we demonstrated that DP1 receptors are also involved in various parameters of human eosinophil activation induced by schistosomal lipids, but not by schistosomal LPC. In addition, schistosomal lipids and their active components PGD2 and LPC, triggered 15-LO dependent production of EXC4 and secretion of TGFβ. Taken together, our results showed that schistosomal lipids contain at least two components-LPC and PGD2-that are capable of direct activation of human eosinophils acting on distinct eosinophil-expressed receptors, noticeably TLR2 as well as DP1, trigger human eosinophil activation characterized by production/secretion of pro-inflammatory and immunoregulatory mediators.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32839/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALKellyG_Magalhães_etal_IOC_2019.pdfKellyG_Magalhães_etal_IOC_2019.pdfapplication/pdf1717602https://www.arca.fiocruz.br/bitstream/icict/32839/2/KellyG_Magalh%c3%a3es_etal_IOC_2019.pdfb7904ecb828587beb8abcd5ff8b25d6aMD52TEXTKellyG_Magalhães_etal_IOC_2019.pdf.txtKellyG_Magalhães_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion
title Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion
spellingShingle Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion
Magalhães, Kelly G.
Eosinófilos
Lipídeos
Schistosoma
Eosinophil
PGD2
Lipids
Schistosoma
TLR
LPC
TGFb
Eoxin
Prostaglandina D2
Fator 88 de Diferenciação Mieloide
title_short Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion
title_full Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion
title_fullStr Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion
title_full_unstemmed Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion
title_sort Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion
author Magalhães, Kelly G.
author_facet Magalhães, Kelly G.
Gomes, Tatiana Luna
Santos, Fabio Mesquita
Corrêa, Rafael
Assunção, Leonardo Santos
Atella, Georgia Correa
Weller, Peter F.
Melo, Christianne Bandeira
Bozza, Patrícia T.
author_role author
author2 Gomes, Tatiana Luna
Santos, Fabio Mesquita
Corrêa, Rafael
Assunção, Leonardo Santos
Atella, Georgia Correa
Weller, Peter F.
Melo, Christianne Bandeira
Bozza, Patrícia T.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Magalhães, Kelly G.
Gomes, Tatiana Luna
Santos, Fabio Mesquita
Corrêa, Rafael
Assunção, Leonardo Santos
Atella, Georgia Correa
Weller, Peter F.
Melo, Christianne Bandeira
Bozza, Patrícia T.
dc.subject.other.pt_BR.fl_str_mv Eosinófilos
Lipídeos
Schistosoma
topic Eosinófilos
Lipídeos
Schistosoma
Eosinophil
PGD2
Lipids
Schistosoma
TLR
LPC
TGFb
Eoxin
Prostaglandina D2
Fator 88 de Diferenciação Mieloide
dc.subject.en.pt_BR.fl_str_mv Eosinophil
PGD2
Lipids
Schistosoma
TLR
LPC
TGFb
Eoxin
dc.subject.decs.pt_BR.fl_str_mv Prostaglandina D2
Fator 88 de Diferenciação Mieloide
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil / Universidade de Brasília. Laboratório de Imunologia e Inflamação. Brasília, DF, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-04-29T16:03:00Z
dc.date.available.fl_str_mv 2019-04-29T16:03:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MAGALHÃES, Kelly G. et al. Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion. Frontiers in Immunology, v. 9. p.1-11, Jan. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32839
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2018.03161
identifier_str_mv MAGALHÃES, Kelly G. et al. Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion. Frontiers in Immunology, v. 9. p.1-11, Jan. 2019.
1664-3224
10.3389/fimmu.2018.03161
url https://www.arca.fiocruz.br/handle/icict/32839
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/32839/2/KellyG_Magalh%c3%a3es_etal_IOC_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/32839/3/KellyG_Magalh%c3%a3es_etal_IOC_2019.pdf.txt
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