Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26056 |
Resumo: | Este trabalho foi apoiado pela FACEPE e pelo PDTIS. |
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Cruz, Heidi Lacerda Alves daMontenegro, Rosana de AlbuquerqueLima, Juliana Falcão de AraújoPoroca, Diogo da RochaLima, Juliana Figueirêdo da CostaMontenegro, Lílian Maria LapaCrovella, SergioSchindler, Haiana Charifker2018-04-24T13:19:28Z2018-04-24T13:19:28Z2011DA CRUZ, H. L. A. et al. Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples. Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology], v. 42, n. 1, p. 321–329, jan. 2011.1517-8382https://www.arca.fiocruz.br/handle/icict/2605610.1590/S1517-83822011000100041Este trabalho foi apoiado pela FACEPE e pelo PDTIS.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Genética. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Genética. Recife, PE, Brasil / University of Trieste. Genetic Service IRCCS Burlo Garofolo. Trieste, Italy.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if not showing a good sensitivity, should be considered as a helpful tool especially in the extrapulmonary cases or in cases where confirmatory diagnosis is quite difficult to be achieved by routine methods. The performance of PCR-based techniques should be considered and tested in future works on other types of biological specimens besides sputum, like blood and urine, readily obtainable in most cases. The improving of M. tuberculosis nested-PCR detection in TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease.engMycobacterium tuberculosisNested-PCRSangueUrinaDiagnóstico molecularSequência de inserção IS6110Mycobacterium tuberculosisNested-PCRBloodUrineIS6110 insertion sequenceMolecular diagnosisReação em Cadeia da Polimerasenested-PCRTuberculose / diagnósticoTuberculose / sangueColeta de Amostras SanguíneasTuberculose / transmissãoBrasil / epidemiologiaHumanosSensibilidade e especificidadeurinaEvaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/26056/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALEVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND.pdfEVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND.pdfapplication/pdf154505https://www.arca.fiocruz.br/bitstream/icict/26056/2/EVALUATION%20OF%20A%20NESTED-PCR%20FOR%20MYCOBACTERIUM%20TUBERCULOSIS%20DETECTION%20IN%20BLOOD%20AND.pdf1081856297766161be40961e96a4c010MD52TEXTEVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND.pdf.txtEVALUATION OF A NESTED-PCR FOR MYCOBACTERIUM TUBERCULOSIS DETECTION IN BLOOD AND.pdf.txtExtracted texttext/plain35496https://www.arca.fiocruz.br/bitstream/icict/26056/3/EVALUATION%20OF%20A%20NESTED-PCR%20FOR%20MYCOBACTERIUM%20TUBERCULOSIS%20DETECTION%20IN%20BLOOD%20AND.pdf.txt6aed217d1d65605740ef260554166598MD53icict/260562018-08-15 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| dc.title.pt_BR.fl_str_mv |
Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples |
| title |
Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples |
| spellingShingle |
Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples Cruz, Heidi Lacerda Alves da Mycobacterium tuberculosis Nested-PCR Sangue Urina Diagnóstico molecular Sequência de inserção IS6110 Mycobacterium tuberculosis Nested-PCR Blood Urine IS6110 insertion sequence Molecular diagnosis Reação em Cadeia da Polimerase nested-PCR Tuberculose / diagnóstico Tuberculose / sangue Coleta de Amostras Sanguíneas Tuberculose / transmissão Brasil / epidemiologia Humanos Sensibilidade e especificidade urina |
| title_short |
Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples |
| title_full |
Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples |
| title_fullStr |
Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples |
| title_full_unstemmed |
Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples |
| title_sort |
Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples |
| author |
Cruz, Heidi Lacerda Alves da |
| author_facet |
Cruz, Heidi Lacerda Alves da Montenegro, Rosana de Albuquerque Lima, Juliana Falcão de Araújo Poroca, Diogo da Rocha Lima, Juliana Figueirêdo da Costa Montenegro, Lílian Maria Lapa Crovella, Sergio Schindler, Haiana Charifker |
| author_role |
author |
| author2 |
Montenegro, Rosana de Albuquerque Lima, Juliana Falcão de Araújo Poroca, Diogo da Rocha Lima, Juliana Figueirêdo da Costa Montenegro, Lílian Maria Lapa Crovella, Sergio Schindler, Haiana Charifker |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cruz, Heidi Lacerda Alves da Montenegro, Rosana de Albuquerque Lima, Juliana Falcão de Araújo Poroca, Diogo da Rocha Lima, Juliana Figueirêdo da Costa Montenegro, Lílian Maria Lapa Crovella, Sergio Schindler, Haiana Charifker |
| dc.subject.other.pt_BR.fl_str_mv |
Mycobacterium tuberculosis Nested-PCR Sangue Urina Diagnóstico molecular Sequência de inserção IS6110 |
| topic |
Mycobacterium tuberculosis Nested-PCR Sangue Urina Diagnóstico molecular Sequência de inserção IS6110 Mycobacterium tuberculosis Nested-PCR Blood Urine IS6110 insertion sequence Molecular diagnosis Reação em Cadeia da Polimerase nested-PCR Tuberculose / diagnóstico Tuberculose / sangue Coleta de Amostras Sanguíneas Tuberculose / transmissão Brasil / epidemiologia Humanos Sensibilidade e especificidade urina |
| dc.subject.en.pt_BR.fl_str_mv |
Mycobacterium tuberculosis Nested-PCR Blood Urine IS6110 insertion sequence Molecular diagnosis |
| dc.subject.decs.pt_BR.fl_str_mv |
Reação em Cadeia da Polimerase nested-PCR Tuberculose / diagnóstico Tuberculose / sangue Coleta de Amostras Sanguíneas Tuberculose / transmissão Brasil / epidemiologia Humanos Sensibilidade e especificidade urina |
| description |
Este trabalho foi apoiado pela FACEPE e pelo PDTIS. |
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2011 |
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2011 |
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2018-04-24T13:19:28Z |
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2018-04-24T13:19:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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DA CRUZ, H. L. A. et al. Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples. Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology], v. 42, n. 1, p. 321–329, jan. 2011. |
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https://www.arca.fiocruz.br/handle/icict/26056 |
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1517-8382 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S1517-83822011000100041 |
| identifier_str_mv |
DA CRUZ, H. L. A. et al. Evaluation of a nested-PCR for mycobacterium tuberculosis detection in blood and urine samples. Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology], v. 42, n. 1, p. 321–329, jan. 2011. 1517-8382 10.1590/S1517-83822011000100041 |
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https://www.arca.fiocruz.br/handle/icict/26056 |
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