Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis

Detalhes bibliográficos
Autor(a) principal: Bernardes-Engemann, A. R.
Data de Publicação: 2005
Outros Autores: Costa, R. C., Miguens, B. R., Penha, C. V., Neves, E., Pereira, B. A., Dias, C. M., Mattos, M., Gutierrez, M. C., Schubach, A., Oliveira Neto, M. P., Lazéra, M. S., Bezerra, L. M. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33249
Resumo: 2020-05-28
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spelling Bernardes-Engemann, A. R.Costa, R. C.Miguens, B. R.Penha, C. V.Neves, E.Pereira, B. A.Dias, C. M.Mattos, M.Gutierrez, M. C.Schubach, A.Oliveira Neto, M. P.Lazéra, M. S.Bezerra, L. M. L.2019-05-28T13:47:47Z2019-05-28T13:47:47Z2005BERNARDES-ENGEMANN, A. R. et al. Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis. Medical Mycology, v. 43, p. 487-493, Sept. 2005.1369-3786https://www.arca.fiocruz.br/handle/icict/3324910.1080/136937804000199091460-2709engOxford University PressDevelopment of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-05-28Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Biologia Celular e Bioquímica de Fungos Patogênicos. Rio de Janeiro, RJ, Brasil.We performed a serological study with sera from 92 patients with confirmed sporotrichosis registered between 1999 and 2004 in two hospitals in Rio de Janeiro State, Brazil. The clinical presentation of sporotrichosis was distributed as follows: lymphocutaneous, 67); fixed cutaneous, 23); disseminated cutaneous, 8); and extracutaneous, 2). Sera were assayed by ELISA against a cell wall antigen of Sporothrix schenckii, SsCBF, that we have previously described. The crossreactivity was determined with 77 heterologous sera. The serological test showed a sensitivity of 90) and a global efficiency of 86). A group of 55 patients with several clinical presentations of sporotrichosis was clinically and serologically followed-up for at least 6 months. We observed by ELISA data a decrease in the antibody serum titers which correlated with the progress in healing. An HIV positive patient with meningeal sporotrichosis was serologically followed-up for over 2 years. Serum and cerebrospinal fluid specimens were examined and significant antibodies levels against the antigen SsCBF were detected. Our results strongly suggest that this serological test is valuable for the differential diagnosis and follow-up of all clinical forms of sporotrichosis.AntigenCell wallS. schenckiiSerodiagnosisSporotrichosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33249/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Bernardes-Engemann_A_etal_INI_2005.pdfve_Bernardes-Engemann_A_etal_INI_2005.pdfapplication/pdf195487https://www.arca.fiocruz.br/bitstream/icict/33249/2/ve_Bernardes-Engemann_A_etal_INI_2005.pdf24416d98dd1ab9e2118661efa6828b62MD52TEXTve_Bernardes-Engemann_A_etal_INI_2005.pdf.txtve_Bernardes-Engemann_A_etal_INI_2005.pdf.txtExtracted texttext/plain30016https://www.arca.fiocruz.br/bitstream/icict/33249/3/ve_Bernardes-Engemann_A_etal_INI_2005.pdf.txt7e724e42d74ac7735f0923a5e0eb1c5bMD53icict/332492021-03-24 16:32:32.344oai:www.arca.fiocruz.br:icict/33249Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:32Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
title Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
spellingShingle Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
Bernardes-Engemann, A. R.
Antigen
Cell wall
S. schenckii
Serodiagnosis
Sporotrichosis
title_short Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
title_full Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
title_fullStr Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
title_full_unstemmed Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
title_sort Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
author Bernardes-Engemann, A. R.
author_facet Bernardes-Engemann, A. R.
Costa, R. C.
Miguens, B. R.
Penha, C. V.
Neves, E.
Pereira, B. A.
Dias, C. M.
Mattos, M.
Gutierrez, M. C.
Schubach, A.
Oliveira Neto, M. P.
Lazéra, M. S.
Bezerra, L. M. L.
author_role author
author2 Costa, R. C.
Miguens, B. R.
Penha, C. V.
Neves, E.
Pereira, B. A.
Dias, C. M.
Mattos, M.
Gutierrez, M. C.
Schubach, A.
Oliveira Neto, M. P.
Lazéra, M. S.
Bezerra, L. M. L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bernardes-Engemann, A. R.
Costa, R. C.
Miguens, B. R.
Penha, C. V.
Neves, E.
Pereira, B. A.
Dias, C. M.
Mattos, M.
Gutierrez, M. C.
Schubach, A.
Oliveira Neto, M. P.
Lazéra, M. S.
Bezerra, L. M. L.
dc.subject.en.pt_BR.fl_str_mv Antigen
Cell wall
S. schenckii
Serodiagnosis
Sporotrichosis
topic Antigen
Cell wall
S. schenckii
Serodiagnosis
Sporotrichosis
description 2020-05-28
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2019-05-28T13:47:47Z
dc.date.available.fl_str_mv 2019-05-28T13:47:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BERNARDES-ENGEMANN, A. R. et al. Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis. Medical Mycology, v. 43, p. 487-493, Sept. 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33249
dc.identifier.issn.pt_BR.fl_str_mv 1369-3786
dc.identifier.doi.none.fl_str_mv 10.1080/13693780400019909
dc.identifier.eissn.none.fl_str_mv 1460-2709
identifier_str_mv BERNARDES-ENGEMANN, A. R. et al. Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis. Medical Mycology, v. 43, p. 487-493, Sept. 2005.
1369-3786
10.1080/13693780400019909
1460-2709
url https://www.arca.fiocruz.br/handle/icict/33249
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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