Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Thais Oliveira
Data de Publicação: 2010
Outros Autores: Costa, Dirceu Joaquim, Brodskyn, Claudia Ida, Duarte, Poliana Mendes, César Neto, João Batista, Nogueira Filho, Getulio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8386
Resumo: UNICAMP. Faculty of Dentistry of Piracicaba. Department of Prosthodontics and Periodontics. São Paulo, SP, Brasil
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spelling Gonçalves, Thais OliveiraCosta, Dirceu JoaquimBrodskyn, Claudia IdaDuarte, Poliana MendesCésar Neto, João BatistaNogueira Filho, Getulio2014-09-16T14:04:52Z2014-09-16T14:04:52Z2010GONÇALVES, T. O. et al. Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis. Archives of Oral Biology, v. 55, n. 12, p. 975-980, 2010.1879-1506https://www.arca.fiocruz.br/handle/icict/838610.1016/j.archoralbio.2010.08.002engElsevier LtdRelease of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUNICAMP. Faculty of Dentistry of Piracicaba. Department of Prosthodontics and Periodontics. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunologia e Parasitologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunologia e Parasitologia. Salvador, BA, BrasilGuarulhos University. Department of Periodontics, Dental Research Division. Guarulhos, São Paulo, BrasilUniversity of São Paulo. School of Dentistry. USP. Department of Periodontology. São Paulo, SP, BrasilUniversity of Manitoba. Faculty of Dentistry. Department of Dental Diagnostic and Surgical Sciences. Division of Periodontics. Bannatyne Ave, Winnipeg, CanadaObjective: The emergence of periodontal medicine increased interest in defining the behaviour of peripheral blood cells in periodontitis subjects in comparison with healthy group. The aim of this study was to evaluate the levels of interleukin (IL)-8, tumour necrosis factora (TNF-a), IL-6 and IL-10 released by Escherichia coli lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) obtained from the peripheral blood of chronic periodontitis subjects. Design: PBMC samples were isolated from 19 systemically healthy donors, divided into generalized chronic periodontitis (n = 10) and healthy (n = 9) subjects. Cells were incubated for 24–48 h in 500 mL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of IL-8, TNF-a, IL-6 and IL-10 released using enzyme-linked immunosorbent assay (ELISA). Results: PBMC cells from periodontitis subjects released higher levels of TNF-a and IL-6 than those from healthy subjects (P < 0.05). Conversely, the supernatants of the stimulated PBMC cells obtained from healthy subjects presented higher amounts of IL-8 than those from periodontitis (P < 0.05). No differences were observed in the levels of IL-10 (P > 0.05) between groups. Conclusion: In conclusion, the results of the present study showed that E. coli LPS-stimulated PBMC from subjects with periodontitis present a different pattern of cytokine release when compared to PBMC from healthy subjects. This phenomenon could have implications locally, in periodontitis, as well as in systemic diseases.PeriodontitisCytokinesELISAMononuclear cellPBMCPeriodontite Crônica/sangueCitocinas/sangueLeucócitos Mononucleares/metabolismoAdultoIdosoCélulas CultivadasEscherichia coliFemininoHemorragia Gengival/sangueHumanosMediadores da Inflamação/sangueInterleucina-10/sangueLeucócitos Mononucleares/efeitos de drogasLipopolissacarídeos/farmacologiaMasculinoMeia-IdadePerda da Inserção Periodontal/sangueBolsa Periodontal/sangueHábito de FumarFator de Necrose Tumoral alfa/sangueinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8386/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALGonçalves TO Release of cytokines.....pdfGonçalves TO Release of cytokines.....pdfapplication/pdf167940https://www.arca.fiocruz.br/bitstream/icict/8386/2/Gon%c3%a7alves%20TO%20Release%20of%20cytokines.....pdf9d65a090db3f919eeee28064257e14f6MD52TEXTGonçalves TO Release of cytokines.....pdf.txtGonçalves TO Release of cytokines.....pdf.txtExtracted texttext/plain28562https://www.arca.fiocruz.br/bitstream/icict/8386/3/Gon%c3%a7alves%20TO%20Release%20of%20cytokines.....pdf.txtc1abc634753e8fbe8fcfa6e1b46f355aMD53icict/83862023-03-15 14:33:12.078oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
title Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
spellingShingle Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
Gonçalves, Thais Oliveira
Periodontitis
Cytokines
ELISA
Mononuclear cell
PBMC
Periodontite Crônica/sangue
Citocinas/sangue
Leucócitos Mononucleares/metabolismo
Adulto
Idoso
Células Cultivadas
Escherichia coli
Feminino
Hemorragia Gengival/sangue
Humanos
Mediadores da Inflamação/sangue
Interleucina-10/sangue
Leucócitos Mononucleares/efeitos de drogas
Lipopolissacarídeos/farmacologia
Masculino
Meia-Idade
Perda da Inserção Periodontal/sangue
Bolsa Periodontal/sangue
Hábito de Fumar
Fator de Necrose Tumoral alfa/sangue
title_short Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
title_full Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
title_fullStr Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
title_full_unstemmed Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
title_sort Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
author Gonçalves, Thais Oliveira
author_facet Gonçalves, Thais Oliveira
Costa, Dirceu Joaquim
Brodskyn, Claudia Ida
Duarte, Poliana Mendes
César Neto, João Batista
Nogueira Filho, Getulio
author_role author
author2 Costa, Dirceu Joaquim
Brodskyn, Claudia Ida
Duarte, Poliana Mendes
César Neto, João Batista
Nogueira Filho, Getulio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gonçalves, Thais Oliveira
Costa, Dirceu Joaquim
Brodskyn, Claudia Ida
Duarte, Poliana Mendes
César Neto, João Batista
Nogueira Filho, Getulio
dc.subject.en.pt_BR.fl_str_mv Periodontitis
Cytokines
ELISA
Mononuclear cell
PBMC
topic Periodontitis
Cytokines
ELISA
Mononuclear cell
PBMC
Periodontite Crônica/sangue
Citocinas/sangue
Leucócitos Mononucleares/metabolismo
Adulto
Idoso
Células Cultivadas
Escherichia coli
Feminino
Hemorragia Gengival/sangue
Humanos
Mediadores da Inflamação/sangue
Interleucina-10/sangue
Leucócitos Mononucleares/efeitos de drogas
Lipopolissacarídeos/farmacologia
Masculino
Meia-Idade
Perda da Inserção Periodontal/sangue
Bolsa Periodontal/sangue
Hábito de Fumar
Fator de Necrose Tumoral alfa/sangue
dc.subject.decs.pt_BR.fl_str_mv Periodontite Crônica/sangue
Citocinas/sangue
Leucócitos Mononucleares/metabolismo
Adulto
Idoso
Células Cultivadas
Escherichia coli
Feminino
Hemorragia Gengival/sangue
Humanos
Mediadores da Inflamação/sangue
Interleucina-10/sangue
Leucócitos Mononucleares/efeitos de drogas
Lipopolissacarídeos/farmacologia
Masculino
Meia-Idade
Perda da Inserção Periodontal/sangue
Bolsa Periodontal/sangue
Hábito de Fumar
Fator de Necrose Tumoral alfa/sangue
description UNICAMP. Faculty of Dentistry of Piracicaba. Department of Prosthodontics and Periodontics. São Paulo, SP, Brasil
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2014-09-16T14:04:52Z
dc.date.available.fl_str_mv 2014-09-16T14:04:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONÇALVES, T. O. et al. Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis. Archives of Oral Biology, v. 55, n. 12, p. 975-980, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8386
dc.identifier.issn.none.fl_str_mv 1879-1506
dc.identifier.doi.none.fl_str_mv 10.1016/j.archoralbio.2010.08.002
identifier_str_mv GONÇALVES, T. O. et al. Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis. Archives of Oral Biology, v. 55, n. 12, p. 975-980, 2010.
1879-1506
10.1016/j.archoralbio.2010.08.002
url https://www.arca.fiocruz.br/handle/icict/8386
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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