Profiling the Trypanosoma cruzi phosphoproteome

Detalhes bibliográficos
Autor(a) principal: Marchini, Fabrício Klerynton
Data de Publicação: 2011
Outros Autores: Godoy, Lyris Martins Franco de, Rampazzo, Rita de Cássia Pontello, Pavoni, Daniela Parada, Probst, Christian Macagnan, Gnad, Florian, Mann, Matthias, Krieger, Marco Aurélio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40085
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
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spelling Marchini, Fabrício KleryntonGodoy, Lyris Martins Franco deRampazzo, Rita de Cássia PontelloPavoni, Daniela ParadaProbst, Christian MacagnanGnad, FlorianMann, MatthiasKrieger, Marco Aurélio2020-02-19T14:39:34Z2020-02-19T14:39:34Z2011MARCHINI, Fabricio K. et al. Profiling the Trypanosoma cruzi Phosphoproteome. Plos One, v. 6, n. 9, p. 1-10, 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/4008510.1371/journal.pone.00253811932-6203porPublic Library of ScienceTrypanosoma cruziKinetoplastidaPhosphorylationProtozoan ProteinsFosforilaciónProteínas ProtozoariasFosforilaçãoProteínas de ProtozoáriosProfiling the Trypanosoma cruzi phosphoproteomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Department of Proteomics and Signal Transduction. Max Planck Institute of Biochemistry. Martinsried, Germany.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Department of Proteomics and Signal Transduction. Max Planck Institute of Biochemistry. Martinsried, Germany.Department of Proteomics and Signal Transduction. Max Planck Institute of Biochemistry. Martinsried, Germany.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Protein phosphorylation is a reversible post-translational modification essential for the regulation of several signal transduction pathways and biological processes in the living cell. Therefore, the identification of protein phosphorylation sites is crucial to understand cell signaling control at the molecular level. Based on mass spectrometry, recent studies have reported the large-scale mapping of phosphorylation sites in various eukaryotes and prokaryotes. However, little is known about the impact of phosphorylation in protozoan parasites. To in depth characterize the phosphoproteome of Trypanosoma cruzi, a parasite of the Kinetoplastida class, protein samples from cells at different phases of the metacyclogenesis--differentiation process of the parasites from non-infective epimastigotes to infective metacyclic trypomastigotes--were enriched for phosphopeptides using TiO(2) chromatography and analyzed on an LTQ-Orbitrap mass spectrometer. In total, 1,671 proteins were identified, including 753 phosphoproteins, containing a total of 2,572 phosphorylation sites. The distribution of phosphorylated residues was 2,162 (84.1%) on serine, 384 (14.9%) on threonine and 26 (1.0%) on tyrosine. Here, we also report several consensus phosphorylation sequence motifs and as some of these conserved groups have enriched biological functions, we can infer the regulation by protein kinases of this functions. To our knowledge, our phosphoproteome is the most comprehensive dataset identified until now for Kinetoplastida species. Here we also were able to extract biological information and infer groups of sites phosphorylated by the same protein kinase. To make our data accessible to the scientific community, we uploaded our study to the data repositories PHOSIDA, Proteome Commons and TriTrypDB enabling researchers to access information about the phosphorylation sites identified here.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/40085/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALjournal.pone.002538ok.PDFjournal.pone.002538ok.PDFapplication/pdf411332https://www.arca.fiocruz.br/bitstream/icict/40085/2/journal.pone.002538ok.PDF1c64dda8f58ad34d9bd06e475d53ef4aMD52TEXTjournal.pone.002538ok.PDF.txtjournal.pone.002538ok.PDF.txtExtracted texttext/plain55275https://www.arca.fiocruz.br/bitstream/icict/40085/3/journal.pone.002538ok.PDF.txta785af0b6edfdb3fef865e2c2d6efdacMD53icict/400852020-02-20 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dc.title.pt_BR.fl_str_mv Profiling the Trypanosoma cruzi phosphoproteome
title Profiling the Trypanosoma cruzi phosphoproteome
spellingShingle Profiling the Trypanosoma cruzi phosphoproteome
Marchini, Fabrício Klerynton
Trypanosoma cruzi
Kinetoplastida
Phosphorylation
Protozoan Proteins
Fosforilación
Proteínas Protozoarias
Fosforilação
Proteínas de Protozoários
title_short Profiling the Trypanosoma cruzi phosphoproteome
title_full Profiling the Trypanosoma cruzi phosphoproteome
title_fullStr Profiling the Trypanosoma cruzi phosphoproteome
title_full_unstemmed Profiling the Trypanosoma cruzi phosphoproteome
title_sort Profiling the Trypanosoma cruzi phosphoproteome
author Marchini, Fabrício Klerynton
author_facet Marchini, Fabrício Klerynton
Godoy, Lyris Martins Franco de
Rampazzo, Rita de Cássia Pontello
Pavoni, Daniela Parada
Probst, Christian Macagnan
Gnad, Florian
Mann, Matthias
Krieger, Marco Aurélio
author_role author
author2 Godoy, Lyris Martins Franco de
Rampazzo, Rita de Cássia Pontello
Pavoni, Daniela Parada
Probst, Christian Macagnan
Gnad, Florian
Mann, Matthias
Krieger, Marco Aurélio
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marchini, Fabrício Klerynton
Godoy, Lyris Martins Franco de
Rampazzo, Rita de Cássia Pontello
Pavoni, Daniela Parada
Probst, Christian Macagnan
Gnad, Florian
Mann, Matthias
Krieger, Marco Aurélio
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Kinetoplastida
topic Trypanosoma cruzi
Kinetoplastida
Phosphorylation
Protozoan Proteins
Fosforilación
Proteínas Protozoarias
Fosforilação
Proteínas de Protozoários
dc.subject.en.pt_BR.fl_str_mv Phosphorylation
Protozoan Proteins
dc.subject.es.pt_BR.fl_str_mv Fosforilación
Proteínas Protozoarias
dc.subject.decs.pt_BR.fl_str_mv Fosforilação
Proteínas de Protozoários
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2020-02-19T14:39:34Z
dc.date.available.fl_str_mv 2020-02-19T14:39:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARCHINI, Fabricio K. et al. Profiling the Trypanosoma cruzi Phosphoproteome. Plos One, v. 6, n. 9, p. 1-10, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40085
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0025381
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv MARCHINI, Fabricio K. et al. Profiling the Trypanosoma cruzi Phosphoproteome. Plos One, v. 6, n. 9, p. 1-10, 2011.
1932-6203
10.1371/journal.pone.0025381
url https://www.arca.fiocruz.br/handle/icict/40085
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/40085/1/license.txt
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