Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin

Detalhes bibliográficos
Autor(a) principal: Portis, Igor Godinho
Data de Publicação: 2020
Outros Autores: Lima, Patrícia de Sousa, Paes, Rodrigo Almeida, Oliveira, Lucas Nojosa, Pereira, Christie Ataídes, Parente-Rocha, Juliana Alves, Pereira, Maristela, Nosanchuk, Joshua Daniel, Soares, Célia Maria de Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/60981
Resumo: This work was supported by grants from Instituto Nacional de Ciência e Tecnologia de Estratégias de Interação Patógeno-Hopedeiro - Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG). CMAS, MP and JAPR are fellow researchers from Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq. JDN is partially supported by NIHAI52733. RAP is supported by CNPq grants (449184/2014-5 and 305487/2015-9). IGP, RAP, LNO and CAP and were supported by fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES. The authors wish to thank Laurine Lacerda Pigosso for the technical assistance in the initial development of the Project.
id CRUZ_ae5bcea67551fda2387301c4d433f0ab
oai_identifier_str oai:www.arca.fiocruz.br:icict/60981
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Portis, Igor GodinhoLima, Patrícia de SousaPaes, Rodrigo AlmeidaOliveira, Lucas NojosaPereira, Christie AtaídesParente-Rocha, Juliana AlvesPereira, MaristelaNosanchuk, Joshua DanielSoares, Célia Maria de Almeida2023-10-29T15:49:19Z2023-10-29T15:49:19Z2020PORTIS, Igor Godinho et al. Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin. Microbiological Research, v. 239, p. 1-9, Oct. 2020.0944-5013https://www.arca.fiocruz.br/handle/icict/6098110.1016/j.micres.2020.1265241618-0623This work was supported by grants from Instituto Nacional de Ciência e Tecnologia de Estratégias de Interação Patógeno-Hopedeiro - Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG). CMAS, MP and JAPR are fellow researchers from Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq. JDN is partially supported by NIHAI52733. RAP is supported by CNPq grants (449184/2014-5 and 305487/2015-9). IGP, RAP, LNO and CAP and were supported by fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES. The authors wish to thank Laurine Lacerda Pigosso for the technical assistance in the initial development of the Project.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brazil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil.Albert Einstein College of Medicine. Department of Medicine. New York City, NY, USA.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Campus II. Laboratório de Biologia Molecular. Goiânia, GO, Brazil.Paracoccidioidomycosis is a highly prevalent systemic mycosis in Latin America, caused by fungi of the genus Paracoccidioides. Copper is essential for eukaryotes and bacteria. This micronutrient is used in many vital biochemical processes, although metal excess levels can be toxic for organisms. Pathways underlying copper overload are poorly understood in members of the Paracoccidioides complex. The responses of Paracoccidioides lutzii yeast cells to copper overload were here evaluated. The results showed that under copper overload, cells presented a dark brown pigment, identified as melanin. Proteomic analyses identified mainly the accumulation of proteins related to amino acids metabolism, ergosterol synthesis and melanin production, suggesting that P. lutzii responds to copper overload by changing aspects of its metabolism and also plasma membrane and cell wall remodeling. Proteomic data were confirmed by biochemical analysis.engElsevierCopper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCellular defenseCopper overloadFungiProteomeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60981/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Igor_Portis_etal_INI_2020.pdfve_Igor_Portis_etal_INI_2020.pdfapplication/pdf1153208https://www.arca.fiocruz.br/bitstream/icict/60981/2/ve_Igor_Portis_etal_INI_2020.pdfe2f9d0c424b793c5897450aed025c524MD52icict/609812023-10-29 12:49:19.965oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-10-29T15:49:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
title Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
spellingShingle Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
Portis, Igor Godinho
Cellular defense
Copper overload
Fungi
Proteome
title_short Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
title_full Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
title_fullStr Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
title_full_unstemmed Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
title_sort Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
author Portis, Igor Godinho
author_facet Portis, Igor Godinho
Lima, Patrícia de Sousa
Paes, Rodrigo Almeida
Oliveira, Lucas Nojosa
Pereira, Christie Ataídes
Parente-Rocha, Juliana Alves
Pereira, Maristela
Nosanchuk, Joshua Daniel
Soares, Célia Maria de Almeida
author_role author
author2 Lima, Patrícia de Sousa
Paes, Rodrigo Almeida
Oliveira, Lucas Nojosa
Pereira, Christie Ataídes
Parente-Rocha, Juliana Alves
Pereira, Maristela
Nosanchuk, Joshua Daniel
Soares, Célia Maria de Almeida
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Portis, Igor Godinho
Lima, Patrícia de Sousa
Paes, Rodrigo Almeida
Oliveira, Lucas Nojosa
Pereira, Christie Ataídes
Parente-Rocha, Juliana Alves
Pereira, Maristela
Nosanchuk, Joshua Daniel
Soares, Célia Maria de Almeida
dc.subject.en.en_US.fl_str_mv Cellular defense
Copper overload
Fungi
Proteome
topic Cellular defense
Copper overload
Fungi
Proteome
description This work was supported by grants from Instituto Nacional de Ciência e Tecnologia de Estratégias de Interação Patógeno-Hopedeiro - Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG). CMAS, MP and JAPR are fellow researchers from Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq. JDN is partially supported by NIHAI52733. RAP is supported by CNPq grants (449184/2014-5 and 305487/2015-9). IGP, RAP, LNO and CAP and were supported by fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES. The authors wish to thank Laurine Lacerda Pigosso for the technical assistance in the initial development of the Project.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2023-10-29T15:49:19Z
dc.date.available.fl_str_mv 2023-10-29T15:49:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PORTIS, Igor Godinho et al. Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin. Microbiological Research, v. 239, p. 1-9, Oct. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/60981
dc.identifier.issn.en_US.fl_str_mv 0944-5013
dc.identifier.doi.none.fl_str_mv 10.1016/j.micres.2020.126524
dc.identifier.eissn.none.fl_str_mv 1618-0623
identifier_str_mv PORTIS, Igor Godinho et al. Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin. Microbiological Research, v. 239, p. 1-9, Oct. 2020.
0944-5013
10.1016/j.micres.2020.126524
1618-0623
url https://www.arca.fiocruz.br/handle/icict/60981
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/60981/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/60981/2/ve_Igor_Portis_etal_INI_2020.pdf
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
e2f9d0c424b793c5897450aed025c524
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324867541499904

Registros relacionados