Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/32921 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil. |
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Souza, Raquel SantosGama, Maiara do Valle FariaSchama, RenataLima, José Bento PereiraDiaz-Albiter, Hector ManuelGenta, Fernando Ariel2019-05-02T17:09:05Z2019-05-02T17:09:05Z2019SOUZA, Raquel Santos et al. Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive b-1,3-Glucanase. Frontiers in Physiology, v. 10, Article 122, 16p, Feb. 2019.1664-042Xhttps://www.arca.fiocruz.br/handle/icict/3292110.3389/fphys.2019.00122engFrontiers MediaAedes aegyptiDigestãoImunidadeFamília da Hidrolase Glicosídica 16Aedes aegyptib-1,3-glucanaseKnock-downDigestionImmunityGlycoside Hydrolase Family 16GHF16Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ. Brasil.University of Glasgow. Wellcome Centre for Molecular Parasitology. Glasgow, United Kingdom.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia para Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Insect β-1,3-glucanases belong to Glycoside Hydrolase Family 16 (GHF16) and are involved in digestion of detritus and plant hemicellulose. In this work, we investigated the role of GHF16 genes in Aedes aegypti larvae, due to their detritivore diet. Aedes aegypti genome has six genes belonging to GHF16 (Aae GH16.1 - Aae GH16.6), containing two to six exons. Sequence analysis suggests that five of these GHF16 sequences (Aae GH16.1, 2, 3, 5, and 6) contain the conserved catalytic residues of this family and correspond to glucanases. All genomes of Nematocera analyzed showed putative gene duplications corresponding to these sequences. Aae GH16.4 has no conserved catalytic residues and is probably a β-1,3-glucan binding protein involved in the activation of innate immune responses. Additionally, Ae. aegypti larvae contain significant β-1,3-glucanase activities in the head, gut and rest of body. These activities have optimum pH about 5-6 and molecular masses between 41 and 150 kDa. All GHF16 genes above showed different levels of expression in the larval head, gut or rest of the body. Knock-down of AeGH16.5 resulted in survival and pupation rates lower than controls (dsGFP and water treated). However, under stress conditions, severe mortalities were observed in AeGH16.1 and AeGH16.6 knocked-down larvae. Enzymatic assays of β-1,3-glucanase in AeGH16.5 silenced larvae exhibited lower activity in the gut and no change in the rest of the body. Chromatographic activity profiles from gut samples after GH16.5 silencing showed suppression of enzymatic activity, suggesting that this gene codes for the digestive larval β-1,3-glucanase of Ae. aegypti. This gene and enzyme are attractive targets for new control strategies, based on the impairment of normal gut physiology.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32921/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRaquelSSouza_MaiaraGama_etal_IOC_2019.pdfRaquelSSouza_MaiaraGama_etal_IOC_2019.pdfapplication/pdf4228770https://www.arca.fiocruz.br/bitstream/icict/32921/2/RaquelSSouza_MaiaraGama_etal_IOC_2019.pdfef33fae21d46b0f7eb4a9d7b5fe2bc8eMD52TEXTRaquelSSouza_MaiaraGama_etal_IOC_2019.pdf.txtRaquelSSouza_MaiaraGama_etal_IOC_2019.pdf.txtExtracted texttext/plain83214https://www.arca.fiocruz.br/bitstream/icict/32921/3/RaquelSSouza_MaiaraGama_etal_IOC_2019.pdf.txt86b6b4a028bb488a2f128bf569dd4392MD53icict/329212019-05-03 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dc.title.pt_BR.fl_str_mv |
Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase |
title |
Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase |
spellingShingle |
Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase Souza, Raquel Santos Aedes aegypti Digestão Imunidade Família da Hidrolase Glicosídica 16 Aedes aegypti b-1,3-glucanase Knock-down Digestion Immunity Glycoside Hydrolase Family 16 GHF16 |
title_short |
Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase |
title_full |
Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase |
title_fullStr |
Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase |
title_full_unstemmed |
Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase |
title_sort |
Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive β-1,3-Glucanase |
author |
Souza, Raquel Santos |
author_facet |
Souza, Raquel Santos Gama, Maiara do Valle Faria Schama, Renata Lima, José Bento Pereira Diaz-Albiter, Hector Manuel Genta, Fernando Ariel |
author_role |
author |
author2 |
Gama, Maiara do Valle Faria Schama, Renata Lima, José Bento Pereira Diaz-Albiter, Hector Manuel Genta, Fernando Ariel |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Raquel Santos Gama, Maiara do Valle Faria Schama, Renata Lima, José Bento Pereira Diaz-Albiter, Hector Manuel Genta, Fernando Ariel |
dc.subject.other.pt_BR.fl_str_mv |
Aedes aegypti Digestão Imunidade Família da Hidrolase Glicosídica 16 |
topic |
Aedes aegypti Digestão Imunidade Família da Hidrolase Glicosídica 16 Aedes aegypti b-1,3-glucanase Knock-down Digestion Immunity Glycoside Hydrolase Family 16 GHF16 |
dc.subject.en.pt_BR.fl_str_mv |
Aedes aegypti b-1,3-glucanase Knock-down Digestion Immunity Glycoside Hydrolase Family 16 GHF16 |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-05-02T17:09:05Z |
dc.date.available.fl_str_mv |
2019-05-02T17:09:05Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUZA, Raquel Santos et al. Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive b-1,3-Glucanase. Frontiers in Physiology, v. 10, Article 122, 16p, Feb. 2019. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/32921 |
dc.identifier.issn.pt_BR.fl_str_mv |
1664-042X |
dc.identifier.doi.none.fl_str_mv |
10.3389/fphys.2019.00122 |
identifier_str_mv |
SOUZA, Raquel Santos et al. Biochemical and Functional Characterization of Glycoside Hydrolase Family 16 Genes in Aedes aegypti Larvae: Identification of the Major Digestive b-1,3-Glucanase. Frontiers in Physiology, v. 10, Article 122, 16p, Feb. 2019. 1664-042X 10.3389/fphys.2019.00122 |
url |
https://www.arca.fiocruz.br/handle/icict/32921 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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