Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity

Bibliographic Details
Main Author: Costa, Jaqueline França
Publication Date: 2012
Other Authors: Wanderley, João Luiz Mendes, Deolindo, Poliana, Zarattini, Jessica B, Costa, Jackson Mauricio Lopes, Soong, Lynn, Barcinski, Marcello André, Barral, Aldina Maria Prado, Borges, Valeria de Matos
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/5844
Summary: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Costa, Jaqueline FrançaWanderley, João Luiz MendesDeolindo, PolianaZarattini, Jessica BCosta, Jackson Mauricio LopesSoong, LynnBarcinski, Marcello AndréBarral, Aldina Maria PradoBorges, Valeria de Matos2012-11-21T17:00:39Z2012-11-21T17:00:39Z2012FRANÇA-COSTA, J. et al. Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity. PLoS One, v. 7, n. 5, p. e36595, 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/584410.1371/journal.pone.0036595engExposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Pólo Universitário Macaé. Rio de Janeiro, RJ, BrasilInstituto Nacional do Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, BrasilInstituto Nacional do Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of Texas Medical Branch. Departments of Microbiology & Immunology and Pathology. Galveston, Texas, United States of AmericaInstituto Nacional do Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilDiffuse cutaneous leishmaniasis (DCL) is a rare clinical manifestation of leishmaniasis, characterized by an inefficient parasite-specific cellular response and heavily parasitized macrophages. In Brazil, Leishmania (Leishmania) amazonensis is the main species involved in DCL cases. In the experimental model, recognition of phosphatidylserine (PS) molecules exposed on the surface of amastigotes forms of L. amazonensis inhibits the inflammatory response of infected macrophages as a strategy to evade the host immune surveillance. In this study, we examined whether PS exposure on L. amazonensis isolates from DCL patients operated as a parasite pathogenic factor and as a putative suppression mechanism of immune response during the infection. Peritoneal macrophages from F1 mice (BALB/c×C57BL/6) were infected with different L. amazonensis isolates from patients with localized cutaneous leishmaniasis (LCL) or DCL. DCL isolates showed higher PS exposure than their counterparts from LCL patients. In addition, PS exposure was positively correlated with clinical parameters of the human infection (number of lesions and time of disease) and with characteristics of the experimental infection (macrophage infection and anti-inflammatory cytokine induction). Furthermore, parasites isolated from DCL patients displayed an increased area in parasitophorous vacuoles (PV) when compared to those isolated from LCL patients. Thus, this study shows for the first time that a parasite factor (exposed PS) might be associated with parasite survival/persistence in macrophages and lesion exacerbation during the course of DCL, providing new insights regarding pathogenic mechanism in this rare chronic disease.Leishmania/efeitos de drogasLeishmania/patogenicidadeLeishmaniose Tegumentar Difusa/parasitologiaFosfatidilserinas/farmacologiaAnimaisDoença CrônicaCitocinas/biossínteseRelação Dose-Resposta a DrogaHumanosTolerância Imunológica/efeitos de drogasLeishmania/isolamento & purificaçãoLeishmaniose Tegumentar Difusa/imunologiaMacrófagos/efeitos de drogasMacrófagos/metabolismoMacrófagos/parasitologiaCamundongosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFrança-Costa J Exposure of phosphatidylserine....pdfFrança-Costa J Exposure of phosphatidylserine....pdfapplication/pdf412815https://www.arca.fiocruz.br/bitstream/icict/5844/1/Fran%c3%a7a-Costa%20J%20Exposure%20of%20phosphatidylserine....pdf53fe07189ee39eaf55c4db8771b86315MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5844/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTFrança-Costa J Exposure of phosphatidylserine....pdf.txtFrança-Costa J Exposure of phosphatidylserine....pdf.txtExtracted texttext/plain52554https://www.arca.fiocruz.br/bitstream/icict/5844/5/Fran%c3%a7a-Costa%20J%20Exposure%20of%20phosphatidylserine....pdf.txtd46802e8b4c88b198624ea67ead281f5MD55THUMBNAILFrança-Costa J Exposure of phosphatidylserine....pdf.jpgFrança-Costa J Exposure of phosphatidylserine....pdf.jpgGenerated Thumbnailimage/jpeg2422https://www.arca.fiocruz.br/bitstream/icict/5844/4/Fran%c3%a7a-Costa%20J%20Exposure%20of%20phosphatidylserine....pdf.jpgc50746d4aea645384cdd9c5277bed9d5MD54icict/58442023-03-15 14:33:26.074oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
title Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
spellingShingle Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
Costa, Jaqueline França
Leishmania/efeitos de drogas
Leishmania/patogenicidade
Leishmaniose Tegumentar Difusa/parasitologia
Fosfatidilserinas/farmacologia
Animais
Doença Crônica
Citocinas/biossíntese
Relação Dose-Resposta a Droga
Humanos
Tolerância Imunológica/efeitos de drogas
Leishmania/isolamento & purificação
Leishmaniose Tegumentar Difusa/imunologia
Macrófagos/efeitos de drogas
Macrófagos/metabolismo
Macrófagos/parasitologia
Camundongos
title_short Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
title_full Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
title_fullStr Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
title_full_unstemmed Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
title_sort Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
author Costa, Jaqueline França
author_facet Costa, Jaqueline França
Wanderley, João Luiz Mendes
Deolindo, Poliana
Zarattini, Jessica B
Costa, Jackson Mauricio Lopes
Soong, Lynn
Barcinski, Marcello André
Barral, Aldina Maria Prado
Borges, Valeria de Matos
author_role author
author2 Wanderley, João Luiz Mendes
Deolindo, Poliana
Zarattini, Jessica B
Costa, Jackson Mauricio Lopes
Soong, Lynn
Barcinski, Marcello André
Barral, Aldina Maria Prado
Borges, Valeria de Matos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Jaqueline França
Wanderley, João Luiz Mendes
Deolindo, Poliana
Zarattini, Jessica B
Costa, Jackson Mauricio Lopes
Soong, Lynn
Barcinski, Marcello André
Barral, Aldina Maria Prado
Borges, Valeria de Matos
dc.subject.decs.pt_BR.fl_str_mv Leishmania/efeitos de drogas
Leishmania/patogenicidade
Leishmaniose Tegumentar Difusa/parasitologia
Fosfatidilserinas/farmacologia
Animais
Doença Crônica
Citocinas/biossíntese
Relação Dose-Resposta a Droga
Humanos
Tolerância Imunológica/efeitos de drogas
Leishmania/isolamento & purificação
Leishmaniose Tegumentar Difusa/imunologia
Macrófagos/efeitos de drogas
Macrófagos/metabolismo
Macrófagos/parasitologia
Camundongos
topic Leishmania/efeitos de drogas
Leishmania/patogenicidade
Leishmaniose Tegumentar Difusa/parasitologia
Fosfatidilserinas/farmacologia
Animais
Doença Crônica
Citocinas/biossíntese
Relação Dose-Resposta a Droga
Humanos
Tolerância Imunológica/efeitos de drogas
Leishmania/isolamento & purificação
Leishmaniose Tegumentar Difusa/imunologia
Macrófagos/efeitos de drogas
Macrófagos/metabolismo
Macrófagos/parasitologia
Camundongos
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2012
dc.date.accessioned.fl_str_mv 2012-11-21T17:00:39Z
dc.date.available.fl_str_mv 2012-11-21T17:00:39Z
dc.date.issued.fl_str_mv 2012
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRANÇA-COSTA, J. et al. Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity. PLoS One, v. 7, n. 5, p. e36595, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5844
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0036595
identifier_str_mv FRANÇA-COSTA, J. et al. Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity. PLoS One, v. 7, n. 5, p. e36595, 2012.
1932-6203
10.1371/journal.pone.0036595
url https://www.arca.fiocruz.br/handle/icict/5844
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/5844/1/Fran%c3%a7a-Costa%20J%20Exposure%20of%20phosphatidylserine....pdf
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