The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener

Detalhes bibliográficos
Autor(a) principal: Santos, Marcia R. M.
Data de Publicação: 1997
Outros Autores: Cano, Maria I., Schijman, Alejandro, Lorenzi, Hernan, Vázquez, Martin, Levin, Mariano J., Ramirez, José Luis, Brandão, Adeilton, Degrave, Wim M., Silveira, José Franco da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36813
Resumo: Universidade Federal de São Paulo. São Paulo, SP, Brasil.
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spelling Santos, Marcia R. M.Cano, Maria I.Schijman, AlejandroLorenzi, HernanVázquez, MartinLevin, Mariano J.Ramirez, José LuisBrandão, AdeiltonDegrave, Wim M.Silveira, José Franco da2019-11-02T19:50:35Z2019-11-02T19:50:35Z1997SANTOS, Marcia R. M. et al. The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 8, p. 821-828, Nov./Dec. 1997.0074-0276https://www.arca.fiocruz.br/handle/icict/3681310.1590/s0074-027619970006000181678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma cruziCone CL BrenerProjeto GenomaMapa cromossômicoMapa genéticoCariótipo molecularTrypanosoma cruziCone CL BrenerGenome projectMolecular karyotypeChromosome mappingGenetic mapThe Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brenerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. São Paulo, SP, Brasil.Universidade Federal de São Paulo. São Paulo, SP, Brasil.Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, INGEBI-CONICET, FCEyN-UBA. Buenos Aires, Argentina.Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, INGEBI-CONICET, FCEyN-UBA. Buenos Aires, Argentina.Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, INGEBI-CONICET, FCEyN-UBA. Buenos Aires, Argentina.Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, INGEBI-CONICET, FCEyN-UBA. Buenos Aires, Argentina.Universidad Central de Venezuela. Caracas, Venezuela.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. São Paulo, SP, Brasil.By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36813/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAdeiltonBrandao_WinDegrave_etal_IOC_1997.pdfAdeiltonBrandao_WinDegrave_etal_IOC_1997.pdfapplication/pdf157316https://www.arca.fiocruz.br/bitstream/icict/36813/2/AdeiltonBrandao_WinDegrave_etal_IOC_1997.pdf0e2a2ef0affe4cd0a69dcbbf1b53d7deMD52TEXTAdeiltonBrandao_WinDegrave_etal_IOC_1997.pdf.txtAdeiltonBrandao_WinDegrave_etal_IOC_1997.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
title The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
spellingShingle The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
Santos, Marcia R. M.
Trypanosoma cruzi
Cone CL Brener
Projeto Genoma
Mapa cromossômico
Mapa genético
Cariótipo molecular
Trypanosoma cruzi
Cone CL Brener
Genome project
Molecular karyotype
Chromosome mapping
Genetic map
title_short The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
title_full The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
title_fullStr The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
title_full_unstemmed The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
title_sort The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
author Santos, Marcia R. M.
author_facet Santos, Marcia R. M.
Cano, Maria I.
Schijman, Alejandro
Lorenzi, Hernan
Vázquez, Martin
Levin, Mariano J.
Ramirez, José Luis
Brandão, Adeilton
Degrave, Wim M.
Silveira, José Franco da
author_role author
author2 Cano, Maria I.
Schijman, Alejandro
Lorenzi, Hernan
Vázquez, Martin
Levin, Mariano J.
Ramirez, José Luis
Brandão, Adeilton
Degrave, Wim M.
Silveira, José Franco da
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Marcia R. M.
Cano, Maria I.
Schijman, Alejandro
Lorenzi, Hernan
Vázquez, Martin
Levin, Mariano J.
Ramirez, José Luis
Brandão, Adeilton
Degrave, Wim M.
Silveira, José Franco da
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Cone CL Brener
Projeto Genoma
Mapa cromossômico
Mapa genético
Cariótipo molecular
topic Trypanosoma cruzi
Cone CL Brener
Projeto Genoma
Mapa cromossômico
Mapa genético
Cariótipo molecular
Trypanosoma cruzi
Cone CL Brener
Genome project
Molecular karyotype
Chromosome mapping
Genetic map
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Cone CL Brener
Genome project
Molecular karyotype
Chromosome mapping
Genetic map
description Universidade Federal de São Paulo. São Paulo, SP, Brasil.
publishDate 1997
dc.date.issued.fl_str_mv 1997
dc.date.accessioned.fl_str_mv 2019-11-02T19:50:35Z
dc.date.available.fl_str_mv 2019-11-02T19:50:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Marcia R. M. et al. The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 8, p. 821-828, Nov./Dec. 1997.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36813
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02761997000600018
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SANTOS, Marcia R. M. et al. The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 8, p. 821-828, Nov./Dec. 1997.
0074-0276
10.1590/s0074-02761997000600018
1678-8060
url https://www.arca.fiocruz.br/handle/icict/36813
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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