Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)

Detalhes bibliográficos
Autor(a) principal: Silva, Jéssica Barreto Lopes
Data de Publicação: 2017
Outros Autores: Alves, Debora Magalhães, Bottino-Rojas, Vanessa, Pereira, Thiago Nunes, Sorgine, Marcos Henrique Ferreira, Caragata, Eric Pearce, Moreira, Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25952
Resumo: Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brazil
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spelling Silva, Jéssica Barreto LopesAlves, Debora MagalhãesBottino-Rojas, VanessaPereira, Thiago NunesSorgine, Marcos Henrique FerreiraCaragata, Eric PearceMoreira, Luciano Andrade2018-04-19T14:40:48Z2018-04-19T14:40:48Z2017SILVA, Jéssica Barreto Lopes et al. Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae). PLoS One, v. 12, n. 7, art. e0181678, 20171932-6203https://www.arca.fiocruz.br/handle/icict/2595210.1371/journal.pone.0181678engPublic Library of ScienceAedes aegyptiSalivadengue virusMosquitoesSalivaAedes aegyptiWolbachiaViral loadDengue virusPathogensWolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, BrazilInstituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, BrazilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrazilInstituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, BrazilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrazilInstituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, BrazilInstituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, BrazilDengue represents a serious threat to human health, with billions of people living at risk of the disease. Wolbachia pipientis is a bacterial endosymbiont common to many insect species. Wolbachia transinfections in mosquito disease vectors have great value for disease control given the bacterium’s ability to spread into wild mosquito populations, and to interfere with infections of pathogens, such as dengue virus. Aedes fluviatilis is a mosquito with a widespread distribution in Latin America, but its status as a dengue vector has not been clarified. Ae. fluviatilis is also naturally infected by the wFlu Wolbachia strain, which has been demonstrated to enhance infection with the avian malarial parasite Plasmodium gallinaceum. We performed experimental infections of Ae. fluviatilis with DENV-2 and DENV-3 isolates from Brazil via injection or oral feeding to provide insight into its competence for the virus. We also examined the effect of the native Wolbachia infection on the virus using a mosquito line where the wFlu infection had been cleared by antibiotic treatment. Through RT-qPCR, we observed that Ae. fluviatilis could become infected with both viruses via either method of infection, although at a lower rate than Aedes aegypti, the primary dengue vector. We then detected DENV-2 and DENV-3 in the saliva of injected mosquitoes, and observed that injection of DENV-3-infected saliva produced subsequent infections in naïve Ae. aegypti. However, across our data we observed no difference in prevalence of infection and viral load between Wolbachia-infected and -uninfected mosquitoes, suggesting that there is no effect of wFlu on dengue virus. Our results highlight that Ae. fluviatilis could potentially serve as a dengue vector under the right circumstances, although further testing is required to determine if this occurs in the fieldinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/25952/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALWolbachia and dengue virus infection.pdfWolbachia and dengue virus infection.pdfapplication/pdf1665142https://www.arca.fiocruz.br/bitstream/icict/25952/2/Wolbachia%20and%20dengue%20virus%20infection.pdf3f740fcee9ffcdcf9f994a2bdb1c398eMD52TEXTWolbachia and dengue virus infection.pdf.txtWolbachia and dengue virus infection.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
spellingShingle Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
Silva, Jéssica Barreto Lopes
Aedes aegypti
Saliva
dengue virus
Mosquitoes
Saliva
Aedes aegypti
Wolbachia
Viral load
Dengue virus
Pathogens
title_short Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_full Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_fullStr Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_full_unstemmed Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
title_sort Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae)
author Silva, Jéssica Barreto Lopes
author_facet Silva, Jéssica Barreto Lopes
Alves, Debora Magalhães
Bottino-Rojas, Vanessa
Pereira, Thiago Nunes
Sorgine, Marcos Henrique Ferreira
Caragata, Eric Pearce
Moreira, Luciano Andrade
author_role author
author2 Alves, Debora Magalhães
Bottino-Rojas, Vanessa
Pereira, Thiago Nunes
Sorgine, Marcos Henrique Ferreira
Caragata, Eric Pearce
Moreira, Luciano Andrade
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Jéssica Barreto Lopes
Alves, Debora Magalhães
Bottino-Rojas, Vanessa
Pereira, Thiago Nunes
Sorgine, Marcos Henrique Ferreira
Caragata, Eric Pearce
Moreira, Luciano Andrade
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Saliva
dengue virus
topic Aedes aegypti
Saliva
dengue virus
Mosquitoes
Saliva
Aedes aegypti
Wolbachia
Viral load
Dengue virus
Pathogens
dc.subject.en.pt_BR.fl_str_mv Mosquitoes
Saliva
Aedes aegypti
Wolbachia
Viral load
Dengue virus
Pathogens
description Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brazil
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-04-19T14:40:48Z
dc.date.available.fl_str_mv 2018-04-19T14:40:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Jéssica Barreto Lopes et al. Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae). PLoS One, v. 12, n. 7, art. e0181678, 2017
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25952
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0181678
identifier_str_mv SILVA, Jéssica Barreto Lopes et al. Wolbachia and dengue virus infection in the mosquito Aedes fluviatilis (Diptera: Culicidae). PLoS One, v. 12, n. 7, art. e0181678, 2017
1932-6203
10.1371/journal.pone.0181678
url https://www.arca.fiocruz.br/handle/icict/25952
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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