Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes

Detalhes bibliográficos
Autor(a) principal: Batista, Cassiano Martin
Data de Publicação: 2018
Outros Autores: Saad, Felipe, Ceccoti, Stephane Pini Costa, Eger, Iriane, Soares, Maurilio José
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28942
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
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spelling Batista, Cassiano MartinSaad, FelipeCeccoti, Stephane Pini CostaEger, IrianeSoares, Maurilio José2018-09-20T15:36:58Z2018-09-20T15:36:58Z2018BATISTA, Cassiano Martin et al. Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n.8, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/2894210.1590/0074-027601800861678-8060porFundação Oswaldo Cruz. Instituto Oswaldo CruzTrypanosoma cruziTrypanosomatinaLipoylationPalmitic AcidGene ExpressionLipoilaciónÁcido PalmíticoExpresión GénicaLipoilaçãoÁcido PalmíticoExpressão GênicaSubcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Universidade Estadual de Ponta Grossa. Departamento de Biologia Geral. Ponta Grossa, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Dynamic S-palmitoylation of proteins is the addition of palmitic acid by zDHHC palmitoyl transferases (PATs) and depalmitoylation by palmitoyl protein thioesterases (PPTs). A putative PAT (TcPAT1) has been previously identified in Trypanosoma cruzi, the etiological agent of Chagas disease. Here we analyse other 14 putative TcPATs and 2 PPTs in the parasite genome. T. cruzi cell lines expressing TcPATs and TcPPTs plus a FLAG tag at the C terminus were produced for most enzymes, with positive detection by indirect immunofluorescence. Overexpressed TcPATs were mostly found as single spots at the parasite anterior end, while the TcPPTs were dispersed throughout the parasite body.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/28942/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALMemoriasIOC-e180086.pdfMemoriasIOC-e180086.pdfapplication/pdf2760826https://www.arca.fiocruz.br/bitstream/icict/28942/2/MemoriasIOC-e180086.pdfbbd52bbab52179eb6b8d08343fd590a5MD52TEXTMemoriasIOC-e180086.pdf.txtMemoriasIOC-e180086.pdf.txtExtracted texttext/plain17962https://www.arca.fiocruz.br/bitstream/icict/28942/3/MemoriasIOC-e180086.pdf.txt2bc96486f606b373081dbe341f3809f9MD53icict/289422018-09-21 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dc.title.pt_BR.fl_str_mv Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
title Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
spellingShingle Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
Batista, Cassiano Martin
Trypanosoma cruzi
Trypanosomatina
Lipoylation
Palmitic Acid
Gene Expression
Lipoilación
Ácido Palmítico
Expresión Génica
Lipoilação
Ácido Palmítico
Expressão Gênica
title_short Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
title_full Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
title_fullStr Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
title_full_unstemmed Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
title_sort Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
author Batista, Cassiano Martin
author_facet Batista, Cassiano Martin
Saad, Felipe
Ceccoti, Stephane Pini Costa
Eger, Iriane
Soares, Maurilio José
author_role author
author2 Saad, Felipe
Ceccoti, Stephane Pini Costa
Eger, Iriane
Soares, Maurilio José
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Batista, Cassiano Martin
Saad, Felipe
Ceccoti, Stephane Pini Costa
Eger, Iriane
Soares, Maurilio José
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Trypanosomatina
topic Trypanosoma cruzi
Trypanosomatina
Lipoylation
Palmitic Acid
Gene Expression
Lipoilación
Ácido Palmítico
Expresión Génica
Lipoilação
Ácido Palmítico
Expressão Gênica
dc.subject.en.pt_BR.fl_str_mv Lipoylation
Palmitic Acid
Gene Expression
dc.subject.es.pt_BR.fl_str_mv Lipoilación
Ácido Palmítico
Expresión Génica
dc.subject.decs.pt_BR.fl_str_mv Lipoilação
Ácido Palmítico
Expressão Gênica
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-09-20T15:36:58Z
dc.date.available.fl_str_mv 2018-09-20T15:36:58Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BATISTA, Cassiano Martin et al. Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n.8, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28942
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760180086
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BATISTA, Cassiano Martin et al. Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n.8, 2018.
0074-0276
10.1590/0074-02760180086
1678-8060
url https://www.arca.fiocruz.br/handle/icict/28942
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/28942/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/28942/3/MemoriasIOC-e180086.pdf.txt
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