Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30015 |
Resumo: | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / School of Life Sciences. University of Nottingham. Nottingham, UK. |
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Silva, Roberta Peres daMartins, Sharon de ToledoRizzo, JulianaReis, Flavia Coelho Garcia dosJoffe, Luna S.Vainstein, Marilene HenningKmetzsch, LíviaOliveira, Débora L.Puccia, RosanaGoldenberg, SamuelRodrigues, Marcio LourençoAlves, Lysangela Ronalte2018-11-13T16:37:36Z2018-11-13T16:37:36Z2018SILVA, Roberta Peres da et al. Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans. Genes, v. 9, n. 8, p. 1-17, 2018.2073-4425https://www.arca.fiocruz.br/handle/icict/3001510.3390/genes90804002073-4425porMDPIGolgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / School of Life Sciences. University of Nottingham. Nottingham, UK.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / School of Life Sciences. University of Nottingham. Nottingham, UK.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Golgi reassembly and stacking protein (GRASP) is required for polysaccharide secretion and virulence in Cryptococcus neoformans. In fungal species, extracellular vesicles (EVs) participate in the export of polysaccharides, proteins and RNA. In the present work, we investigated if EV-mediated RNA export is functionally connected with GRASP in C. neoformans using a graspΔ mutant. Since GRASP-mediated unconventional secretion involves autophagosome formation in yeast, we included the atg7Δ mutant with defective autophagic mechanisms in our analysis. All fungal strains exported EVs but deletion of GRASP or ATG7 profoundly affected vesicular dimensions. The mRNA content of the graspΔ EVs differed substantially from that of the other two strains. The transcripts associated to the endoplasmic reticulum were highly abundant transcripts in graspΔ EVs. Among non-coding RNAs (ncRNAs), tRNA fragments were the most abundant in both mutant EVs but graspΔ EVs alone concentrated 22 exclusive sequences. In general, our results showed that the EV RNA content from atg7Δ and WT were more related than the RNA content of graspΔ, suggesting that GRASP, but not the autophagy regulator Atg7, is involved in the EV export of RNA. This is a previously unknown function for a key regulator of unconventional secretion in eukaryotic cells.Extracellular VesiclesGolgi ApparatusAutophagy-Related Protein 7Secretory PathwayARNAparato de GolgiProteína 7 Relacionada con la AutofagiaVías SecretorasCryptococcus neoformansRNAVesículas ExtracelularesComplexo de GolgiProteína 7 Relacionada à AutofagiaVia Secretóriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/30015/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALgenes-09-004.pdfgenes-09-004.pdfapplication/pdf12247558https://www.arca.fiocruz.br/bitstream/icict/30015/2/genes-09-004.pdfa25ce07bb830b129825ea34ac1db3fd1MD52TEXTgenes-09-004.pdf.txtgenes-09-004.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans |
title |
Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans |
spellingShingle |
Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans Silva, Roberta Peres da Extracellular Vesicles Golgi Apparatus Autophagy-Related Protein 7 Secretory Pathway ARN Aparato de Golgi Proteína 7 Relacionada con la Autofagia Vías Secretoras Cryptococcus neoformans RNA Vesículas Extracelulares Complexo de Golgi Proteína 7 Relacionada à Autofagia Via Secretória |
title_short |
Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans |
title_full |
Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans |
title_fullStr |
Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans |
title_full_unstemmed |
Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans |
title_sort |
Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans |
author |
Silva, Roberta Peres da |
author_facet |
Silva, Roberta Peres da Martins, Sharon de Toledo Rizzo, Juliana Reis, Flavia Coelho Garcia dos Joffe, Luna S. Vainstein, Marilene Henning Kmetzsch, Lívia Oliveira, Débora L. Puccia, Rosana Goldenberg, Samuel Rodrigues, Marcio Lourenço Alves, Lysangela Ronalte |
author_role |
author |
author2 |
Martins, Sharon de Toledo Rizzo, Juliana Reis, Flavia Coelho Garcia dos Joffe, Luna S. Vainstein, Marilene Henning Kmetzsch, Lívia Oliveira, Débora L. Puccia, Rosana Goldenberg, Samuel Rodrigues, Marcio Lourenço Alves, Lysangela Ronalte |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Roberta Peres da Martins, Sharon de Toledo Rizzo, Juliana Reis, Flavia Coelho Garcia dos Joffe, Luna S. Vainstein, Marilene Henning Kmetzsch, Lívia Oliveira, Débora L. Puccia, Rosana Goldenberg, Samuel Rodrigues, Marcio Lourenço Alves, Lysangela Ronalte |
dc.subject.en.pt_BR.fl_str_mv |
Extracellular Vesicles Golgi Apparatus Autophagy-Related Protein 7 Secretory Pathway |
topic |
Extracellular Vesicles Golgi Apparatus Autophagy-Related Protein 7 Secretory Pathway ARN Aparato de Golgi Proteína 7 Relacionada con la Autofagia Vías Secretoras Cryptococcus neoformans RNA Vesículas Extracelulares Complexo de Golgi Proteína 7 Relacionada à Autofagia Via Secretória |
dc.subject.es.pt_BR.fl_str_mv |
ARN Aparato de Golgi Proteína 7 Relacionada con la Autofagia Vías Secretoras |
dc.subject.decs.pt_BR.fl_str_mv |
Cryptococcus neoformans RNA Vesículas Extracelulares Complexo de Golgi Proteína 7 Relacionada à Autofagia Via Secretória |
description |
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / School of Life Sciences. University of Nottingham. Nottingham, UK. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-11-13T16:37:36Z |
dc.date.available.fl_str_mv |
2018-11-13T16:37:36Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Roberta Peres da et al. Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans. Genes, v. 9, n. 8, p. 1-17, 2018. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/30015 |
dc.identifier.issn.pt_BR.fl_str_mv |
2073-4425 |
dc.identifier.doi.none.fl_str_mv |
10.3390/genes9080400 |
dc.identifier.eissn.none.fl_str_mv |
2073-4425 |
identifier_str_mv |
SILVA, Roberta Peres da et al. Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans. Genes, v. 9, n. 8, p. 1-17, 2018. 2073-4425 10.3390/genes9080400 |
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https://www.arca.fiocruz.br/handle/icict/30015 |
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