Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides

Detalhes bibliográficos
Autor(a) principal: Pessoa, Grasielle Caldas D Ávila
Data de Publicação: 2015
Outros Autores: Pinheiro, Letícia Cavalari, Ferraz, Marcela Lencine, Mello, Bernardino Vaz de, Diotaiuti, Liléia Gonçalves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12631
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, Brasil.
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spelling Pessoa, Grasielle Caldas D ÁvilaPinheiro, Letícia CavalariFerraz, Marcela LencineMello, Bernardino Vaz deDiotaiuti, Liléia Gonçalves2016-01-27T12:25:47Z2016-01-27T12:25:47Z2015PESSOA, Grasielle Caldas D Ávila et al. Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides. Parasites & Vectors, v. 8, p. 1-5, 2015.1756-3305https://www.arca.fiocruz.br/handle/icict/1263110.1186/s13071-015-0726-4engBioMed CentralStandardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, Brasil.Secretaria de Saúde do Estado de Minas Gerais. Belo Horizonte, MG, Brasil.Secretaria de Saúde do Estado de Minas Gerais. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, Brasil.Background: Increasing reports of high-resistant Triatominae populations concerns scientists and sanitarians as little is known about the factors behind the occurrence of such phenotype and its real impact on vector control strategies. Moreover, the utilization of a large variety of methodologies hinder the comparison of the reported studies. Methods: This work aims to review laboratory bioassays, redefining the assessed biological features (age, generation and insecticide application area) and technical procedures (mortality recording time and the ideal diagnostic dose). Results: Results were not influenced by the insecticide application area in nymphs or by their generation. Three days-old specimen’s revealed lower susceptibility to the tested insecticide. We determined that it is more appropriate to record mortality 72 h after treatment with insecticide, as well as using a diagnostic dose of 1xDL99. Conclusion: This work suggests more adequate methodological parameters for assessing insecticide resistance in triatomines, which also allows the comparison of results obtained by different research groups. For laboratory bioassays, we recommend: 1) the use of first instar nymphs from first or second generation; 2) 3 day-old specimens; 2) application of insecticide in the dorsal or ventral abdomen area; 3) mortality recording 72 h after treatment with pyrethroids and 4) a diagnostic dose of 1x LD99.Insecticide resistanceBioassaysTriatominaeTriatoma sordidainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12631/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALStandardization of laboratory bioassays for the study of Triatoma sordida .pdfStandardization of laboratory bioassays for the study of Triatoma sordida 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dc.title.pt_BR.fl_str_mv Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
title Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
spellingShingle Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
Pessoa, Grasielle Caldas D Ávila
Insecticide resistance
Bioassays
Triatominae
Triatoma sordida
title_short Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
title_full Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
title_fullStr Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
title_full_unstemmed Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
title_sort Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
author Pessoa, Grasielle Caldas D Ávila
author_facet Pessoa, Grasielle Caldas D Ávila
Pinheiro, Letícia Cavalari
Ferraz, Marcela Lencine
Mello, Bernardino Vaz de
Diotaiuti, Liléia Gonçalves
author_role author
author2 Pinheiro, Letícia Cavalari
Ferraz, Marcela Lencine
Mello, Bernardino Vaz de
Diotaiuti, Liléia Gonçalves
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pessoa, Grasielle Caldas D Ávila
Pinheiro, Letícia Cavalari
Ferraz, Marcela Lencine
Mello, Bernardino Vaz de
Diotaiuti, Liléia Gonçalves
dc.subject.en.pt_BR.fl_str_mv Insecticide resistance
Bioassays
Triatominae
Triatoma sordida
topic Insecticide resistance
Bioassays
Triatominae
Triatoma sordida
description Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-27T12:25:47Z
dc.date.available.fl_str_mv 2016-01-27T12:25:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PESSOA, Grasielle Caldas D Ávila et al. Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides. Parasites & Vectors, v. 8, p. 1-5, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12631
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-015-0726-4
identifier_str_mv PESSOA, Grasielle Caldas D Ávila et al. Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides. Parasites & Vectors, v. 8, p. 1-5, 2015.
1756-3305
10.1186/s13071-015-0726-4
url https://www.arca.fiocruz.br/handle/icict/12631
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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