CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model

Detalhes bibliográficos
Autor(a) principal: Pérez, George V.
Data de Publicação: 2023
Outros Autores: Rosales, Mauro, Ramón, Ailyn C., Rodríguez-Ulloa, Arielis, Besada, Vladimir, González, Luis J., Aguilar, Daylen, Vázquez-Blomquist, Dania, Falcón, Viviana, Caballero, Evelin, Carvalho, Paulo Costa, Caldeira, Rodrigo Soares, Yang, Ke, Perera, Yasser, Perea, Silvio E.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57177
Resumo: Molecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.
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spelling Pérez, George V.Rosales, MauroRamón, Ailyn C.Rodríguez-Ulloa, ArielisBesada, VladimirGonzález, Luis J.Aguilar, DaylenVázquez-Blomquist, DaniaFalcón, VivianaCaballero, EvelinCarvalho, Paulo CostaCaldeira, Rodrigo SoaresYang, KePerera, YasserPerea, Silvio E.2023-03-02T18:43:06Z2023-03-02T18:43:06Z2023PÉREZ, George V. et al. CIGB-300 Anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model. Biomedicines, v. 11, n. 43, p. 1–16, 2023.2227-9059https://www.arca.fiocruz.br/handle/icict/5717710.3390/ biomedicines11010043porMDPICIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMolecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.Molecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba. / Department of Animal and Human Biology. Faculty of Biology. University of Havana (UH). Havana, Cuba.Molecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.Mass Spectrometry Laboratory. Proteomics Group. Department of System Biology. Biomedical Research Division. CIGB. Havana, Cuba.Mass Spectrometry Laboratory. Proteomics Group. Department of System Biology. Biomedical Research Division. CIGB. Havana, Cuba.Mass Spectrometry Laboratory. Proteomics Group. Department of System Biology. Biomedical Research Division. CIGB. Havana, Cuba.Molecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.Pharmacogenomic Group. Department of System Biology. Biomedical Research Division. CIGB. Havana, Cuba.Microscopy Laboratory. Department of System Biology. Biomedical Research Division. CIGB. Havana, Cuba.Molecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Mass Spectrometry Facility. Curitiba, PR, Brasil.China-Cuba Biotechnology Joint Innovation Center (CCBJIC). Yongzhou Zhong Gu Biotechnology. Yongzhou, China.Molecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba. / China-Cuba Biotechnology Joint Innovation Center (CCBJIC). Yongzhou Zhong Gu Biotechnology. Yongzhou, China.Molecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.Large cell lung carcinoma (LCLC) is one form of NSCLC that spreads more aggressively than some other forms, and it represents an unmet medical need. Here, we investigated for the first time the effect of the anti-CK2 CIGB-300 peptide in NCI-H460 cells as an LCLC model. NCI-H460 cells were highly sensitive toward CIGB-300 cytotoxicity, reaching a peak of apoptosis at 6 h. Moreover, CIGB-300 slightly impaired the cell cycle of NCI-H460 cells. The CIGB-300 interactomics profile revealed in more than 300 proteins that many of them participated in biological processes relevant in cancer. Interrogation of the CK2 subunits targeting by CIGB-300 indicated the higher binding of the peptide to the CK2 0 catalytic subunit by in vivo pull-down assays plus immunoblotting analysis and confocal microscopy. The down-regulation of both phosphorylation and protein levels of the ribonuclear protein S6 (RPS6) was observed 48 h post treatment. altogether, we have found that NCI-H460 cells are the most CIGB-300-sensitive solid tumor cell line described so far, and also, the findings we provide here uncover novel features linked to CK2 targeting by the CIGB-300 anticancer peptide.Lung CancerLCLCLarge Cell Lung CarcinomaCarcinome à grandes cellulesNeoplasias PulmonaresCâncer de PulmãoCarcinoma de Células Grandesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model
title CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model
spellingShingle CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model
Pérez, George V.
Lung Cancer
LCLC
Large Cell Lung Carcinoma
Carcinome à grandes cellules
Neoplasias Pulmonares
Câncer de Pulmão
Carcinoma de Células Grandes
title_short CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model
title_full CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model
title_fullStr CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model
title_full_unstemmed CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model
title_sort CIGB-300 anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model
author Pérez, George V.
author_facet Pérez, George V.
Rosales, Mauro
Ramón, Ailyn C.
Rodríguez-Ulloa, Arielis
Besada, Vladimir
González, Luis J.
Aguilar, Daylen
Vázquez-Blomquist, Dania
Falcón, Viviana
Caballero, Evelin
Carvalho, Paulo Costa
Caldeira, Rodrigo Soares
Yang, Ke
Perera, Yasser
Perea, Silvio E.
author_role author
author2 Rosales, Mauro
Ramón, Ailyn C.
Rodríguez-Ulloa, Arielis
Besada, Vladimir
González, Luis J.
Aguilar, Daylen
Vázquez-Blomquist, Dania
Falcón, Viviana
Caballero, Evelin
Carvalho, Paulo Costa
Caldeira, Rodrigo Soares
Yang, Ke
Perera, Yasser
Perea, Silvio E.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pérez, George V.
Rosales, Mauro
Ramón, Ailyn C.
Rodríguez-Ulloa, Arielis
Besada, Vladimir
González, Luis J.
Aguilar, Daylen
Vázquez-Blomquist, Dania
Falcón, Viviana
Caballero, Evelin
Carvalho, Paulo Costa
Caldeira, Rodrigo Soares
Yang, Ke
Perera, Yasser
Perea, Silvio E.
dc.subject.en.en_US.fl_str_mv Lung Cancer
LCLC
Large Cell Lung Carcinoma
topic Lung Cancer
LCLC
Large Cell Lung Carcinoma
Carcinome à grandes cellules
Neoplasias Pulmonares
Câncer de Pulmão
Carcinoma de Células Grandes
dc.subject.fr.en_US.fl_str_mv Carcinome à grandes cellules
dc.subject.decs.en_US.fl_str_mv Neoplasias Pulmonares
Câncer de Pulmão
Carcinoma de Células Grandes
description Molecular Oncology Group. Department of Pharmaceuticals. Biomedical Research Division. Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-03-02T18:43:06Z
dc.date.available.fl_str_mv 2023-03-02T18:43:06Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PÉREZ, George V. et al. CIGB-300 Anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model. Biomedicines, v. 11, n. 43, p. 1–16, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57177
dc.identifier.issn.en_US.fl_str_mv 2227-9059
dc.identifier.doi.none.fl_str_mv 10.3390/ biomedicines11010043
identifier_str_mv PÉREZ, George V. et al. CIGB-300 Anticancer peptide differentially interacts with CK2 subunits and regulates specific signaling mediators in a highly sensitive large cell lung carcinoma cell model. Biomedicines, v. 11, n. 43, p. 1–16, 2023.
2227-9059
10.3390/ biomedicines11010043
url https://www.arca.fiocruz.br/handle/icict/57177
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57177/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/57177/2/biomedicines-11-00043.pdf
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