Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs

Detalhes bibliográficos
Autor(a) principal: Menezes, Rodrigo C.
Data de Publicação: 2013
Outros Autores: Figueiredo, Fabiano B., Wise, Annabel G., Madeira, Maria F., Oliveira, Raquel V. C., Schubach, Tânia M. P., Kiupel, Matti, Langohr, Ingeborg M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28802
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
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spelling Menezes, Rodrigo C.Figueiredo, Fabiano B.Wise, Annabel G.Madeira, Maria F.Oliveira, Raquel V. C.Schubach, Tânia M. P.Kiupel, MattiLangohr, Ingeborg M.2018-09-14T13:59:06Z2018-09-14T13:59:06Z2013MENEZES, Rodrigo C. et al. Sensitivity and Specificity of In Situ Hybridization for Diagnosis of Cutaneous Infection by Leishmania infantum in Dogs. Journal of Clinical Microbiology, v. 51, n. 1, p. 206-211, 2013.1098-660Xhttps://www.arca.fiocruz.br/handle/icict/2880210.1128/JCM.02123-12engSensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Michigan State University. College of Veterinary Medicine. Diagnostic Center for Population and Animal Health. Lansing, Michigan, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Michigan State University. College of Veterinary Medicine. Diagnostic Center for Population and Animal Health. Lansing, Michigan, USA.Michigan State University. College of Veterinary Medicine. Diagnostic Center for Population and Animal Health. Lansing, Michigan, USA.An accurate diagnosis of infection by Leishmania infantum in dogs is fundamental for the control of zoonotic visceral leishmaniasis (VL). Histopathology (HP) and immunohistochemistry (IHC) are frequently used for the histological diagnosis of L. infantum in dogs but have shown limited accuracy. To improve the sensitivity and specificity of the histological diagnosis of VL, we evaluated automated in situ hybridization (ISH) using a generic probe for Leishmania and a specific probe for L. infantum in surgical skin biopsy specimens of dogs. The ISH results were compared with those of HP and IHC, using parasitological culture as the reference standard. Skin samples from 51 dogs with cutaneous L. infantum infection and 51 noninfected dogs were randomly selected from samples of dogs from various cities in Brazil where canine VL is endemic. These samples were processed for parasitological culture, HP, IHC, and ISH using both probes. The sensitivities of ISH using the specific probe, ISH using the generic probe, IHC, and HP were, respectively, 74.5%, 70.6%, 69.5%, and 57.6%. The specificity of both ISH probes tested was 100%, and there was no cross-hybridization of the generic and specific probes with selected pathogenic fungi and protozoa. The specific probe discriminated L. infantum from the other species of Leishmania that infect dogs in the New World. ISH is highly sensitive and specific for the diagnosis of L. infantum in histologic samples of skin from infected dogs and can be used on routine biopsy material to make a diagnosis of leishmaniasis.Leishmania infantumLeishmaniasis, VisceralCãesLeishmaniasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83100https://www.arca.fiocruz.br/bitstream/icict/28802/1/license.txte0c0e718db411cbd1e3f91252b389ec2MD51ORIGINALJCM.02123-12.full.pdfJCM.02123-12.full.pdfapplication/pdf4907835https://www.arca.fiocruz.br/bitstream/icict/28802/2/JCM.02123-12.full.pdfea2cad885627c23b1175ab683c2b7ff0MD52TEXTJCM.02123-12.full.pdf.txtJCM.02123-12.full.pdf.txtExtracted texttext/plain40769https://www.arca.fiocruz.br/bitstream/icict/28802/3/JCM.02123-12.full.pdf.txt535d021d2e05978fc286b74d3bcc748cMD53icict/288022018-09-20 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dc.title.pt_BR.fl_str_mv Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs
title Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs
spellingShingle Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs
Menezes, Rodrigo C.
Leishmania infantum
Leishmaniasis, Visceral
Cães
Leishmaniasis
title_short Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs
title_full Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs
title_fullStr Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs
title_full_unstemmed Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs
title_sort Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs
author Menezes, Rodrigo C.
author_facet Menezes, Rodrigo C.
Figueiredo, Fabiano B.
Wise, Annabel G.
Madeira, Maria F.
Oliveira, Raquel V. C.
Schubach, Tânia M. P.
Kiupel, Matti
Langohr, Ingeborg M.
author_role author
author2 Figueiredo, Fabiano B.
Wise, Annabel G.
Madeira, Maria F.
Oliveira, Raquel V. C.
Schubach, Tânia M. P.
Kiupel, Matti
Langohr, Ingeborg M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Menezes, Rodrigo C.
Figueiredo, Fabiano B.
Wise, Annabel G.
Madeira, Maria F.
Oliveira, Raquel V. C.
Schubach, Tânia M. P.
Kiupel, Matti
Langohr, Ingeborg M.
dc.subject.en.pt_BR.fl_str_mv Leishmania infantum
Leishmaniasis, Visceral
Cães
Leishmaniasis
topic Leishmania infantum
Leishmaniasis, Visceral
Cães
Leishmaniasis
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2018-09-14T13:59:06Z
dc.date.available.fl_str_mv 2018-09-14T13:59:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MENEZES, Rodrigo C. et al. Sensitivity and Specificity of In Situ Hybridization for Diagnosis of Cutaneous Infection by Leishmania infantum in Dogs. Journal of Clinical Microbiology, v. 51, n. 1, p. 206-211, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28802
dc.identifier.issn.pt_BR.fl_str_mv 1098-660X
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.02123-12
identifier_str_mv MENEZES, Rodrigo C. et al. Sensitivity and Specificity of In Situ Hybridization for Diagnosis of Cutaneous Infection by Leishmania infantum in Dogs. Journal of Clinical Microbiology, v. 51, n. 1, p. 206-211, 2013.
1098-660X
10.1128/JCM.02123-12
url https://www.arca.fiocruz.br/handle/icict/28802
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/28802/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/28802/2/JCM.02123-12.full.pdf
https://www.arca.fiocruz.br/bitstream/icict/28802/3/JCM.02123-12.full.pdf.txt
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